Council for Research and Experimentation in Agriculture

Scafati, Italy

Council for Research and Experimentation in Agriculture

Scafati, Italy
SEARCH FILTERS
Time filter
Source Type

Buommino E.,Section of Microbiology and Clinical Microbiology | Tirino V.,Italian National Cancer Institute | de Filippis A.,Section of Microbiology and Clinical Microbiology | Silvestri F.,Section of Microbiology and Clinical Microbiology | And 4 more authors.
Cell Proliferation | Year: 2011

Objectives: Cancer stem cells make up a subpopulation of cells within tumours that drive tumour initiation, growth and recurrence. They are resistant to many current types of cancer treatment, causing failure of such therapeutic approaches, including chemotherapy and radiotherapy. In the study described here, anti-proliferative effects of 3-O-methylfunicone (OMF), a metabolite from Penicillium pinophilum, were investigated on human breast cancer MCF-7 cells and cancer stem cells selected as mammospheres derived from MCF-7s. Materials and methods: Stemness markers were analysed on isolated mammospheres showing positive expression of CD24, CD29, CD44, CD133, CD184 and CD338. Cell proliferation and apoptosis were analysed by flow cytometry and RT-PCR. Cell colony formation assays were performed to evaluate colony formation of mammospheres. Results and conclusion: OMF treatment affected both MCF-7 and mammosphere growth, inducing apoptosis. In addition, OMF strongly reduced stemness markers and survivin, hTERT and Nanog-1 gene expression. Growth of colonies in soft-agar was significantly affected by OMF treatment, too. Lastly, we tested ability of MCF-7 cells to form mammospheres after treatment with OMF or cisplatin, demonstrating that OMF treatment resulted in drastic reduction in number of mammospheres. These results introduce OMF as an effective molecule in suppressing breast cancer stem cells. © 2011 Blackwell Publishing Ltd.


Acciarri N.,Council for Research and Experimentation in Agriculture | Sabatini E.,Council for Research and Experimentation in Agriculture | Ciriaci T.,Council for Research and Experimentation in Agriculture | Rotino L.G.,Council for Research and Experimentation in Agriculture | And 2 more authors.
European Journal of Horticultural Science | Year: 2010

A sample of about 169 tomato landraces of varying fruit type and of diverse provenance was tested for reaction to infection with Verticillium dahliae, the causative pathogen of Verticillium wilt. Some individuals of the 'Sorrentino' landrace were as resistant as 'Florida Ve', whereas certain populations of the 'Ligure', rather known as 'Cuor di bue di Albenga', type included the extremes of susceptibility and resistance. Resistant selections from each landrace were allowed to self fertilize to generate breeding lines. A PCR analysis, based on a CAPS marker targeted to the Ve2 allele, was conducted on 'Sorrentino', 'Locale di Albenga 2', 'Locale di Albenga 3', 'Locale di Carmagnola', 'Locale di Cuneo', and 'Eraldo4', and the resulting genotype was fully consistent with reaction to infection with V. dahliae strain V7. Variation in the level of resistance among Ve2 carriers suggested that other genes are also involved in modulating the response to infection. Markers for resistant alleles at Ve should prove useful in marker assisted breeding for wilt resistance in tomato. Homozygous resistant selections were made from land-race materials, which retained the landrace fruit type. © Verlag Eugen Ulmer KG, Stuttgart.


Buommino E.,The Second University of Naples | Paoletti I.,The Second University of Naples | De Filippis A.,The Second University of Naples | Nicoletti R.,Council for Research and Experimentation in Agriculture | And 4 more authors.
Cell Proliferation | Year: 2010

Objectives: 3-O-methylfunicone (OMF), a secondary metabolite produced by Penicillium pinophilum, affects cell proliferation and motility in a variety of human solid tumours. The aim of this study was to demonstrate whether OMF has the ability to arrest cell division and motility, in a human mesothelioma cell line. Malignant mesothelioma is an aggressive cancer that does not respond to standard therapies the cells of which are considered to be highly resistant to apoptosis. Material and methods: Cell motility and invasion were measured using a modified Boyden chamber. Gene expression was examined by RT-PCR, while ERK1/2 was investigated by Western blot analysis. All experiments were also performed on primary cultures of mesothelial cells. Results: The present study shows that OMF inhibited motility of the NCI mesothelioma cell line by modulating ERK signalling activity, and affected αVβ5 integrin and MMP-2 expression, inducing marked downregulation at both mRNA and protein levels. Substantial downregulation of VEGF gene expression was also demonstrated. These effects were not observed in normal mesothelial cell cultures. Conclusion: OMF may have potential as a naturally derived anti-tumour drug for treatment of mesothelioma. © 2010 Blackwell Publishing Ltd.


Buommino E.,The Second University of Naples | De Filippis A.,The Second University of Naples | Nicoletti R.,Council for Research and Experimentation in Agriculture | Menegozzo M.,Section of Job Medicine | And 6 more authors.
Investigational New Drugs | Year: 2012

Malignant pleural mesothelioma is a fatal malignancy linked to asbestos exposure. The main challenge for mesothelioma treatment is to go beyond the drug resistance, in particular against cisplatin (CDDP), one of the most used chemotherapeutic drug. 3-O-methylfunicone (OMF) is a metabolite produced by the fungus Penicillium pinophilum; its antiproliferative properties have been previously studied in vitro. Particularly, OMF is able to inhibit mesothelioma cell motility. To improve the effects of CDDP by-passing the resistance of mesothelioma cells to this drug, in the present study we investigated the combined treatment of OMF with CDDP respectively in an established mesothelioma cell line (NCI) and primary mesothelioma cells (Mest). As compared to the effect of single treatments, the combination of OMF and CDDP resulted in a stronger inhibition of NCI and Mest cell proliferation. OMF combination with CDDP was also able to affect the migratory ability of NCI and Mest cells by down-regulating αv and β5 expression and reducing metalloproteinase 2 (MMP-2) production. In addition, this association was effective in modulating VEGF gene expression. This finding highlights the possibility to use OMF and CDDP together to regulate angiogenesis and tumour progression in mesothelioma. © Springer Science+Business Media, LLC 2011.


Nicoletti R.,Council for Research and Experimentation in Agriculture | Buommino E.,The Second University of Naples | Tufano M.A.,The Second University of Naples
Recent Patents on Biotechnology | Year: 2012

Penicillium species are a widespread source of biologically active compounds and enzymes which are exploited in biotechnologies. The ongoing discovery of new species, their biochemical and molecular characterization, and the application of the new findings in diverse industrial processes stimulate an increasing interest of patentees worldwide. An overview of the patents released in the last four years in agriculture, bioremediation, and in several industrial fields for the production of biofuels, food, cosmetics and pharmaceuticals is proposed for an exhaustive appreciation of the potential cues offered to inventors by these fungi. © 2012 Bentham Science Publishers.


Caroprese M.,University of Foggia | Ciliberti M.G.,University of Foggia | Annicchiarico G.,Council for Research and Experimentation in Agriculture | Albenzio M.,University of Foggia | And 2 more authors.
Journal of Dairy Science | Year: 2014

The aim of this study was to assess the effects of supplementation with polyunsaturated fatty acids from different sources on immune regulation and hypothalamic-pituitary-adrenal (HPA) axis activation in heat-stressed sheep. The experiment was carried out during the summer 2012. Thirty-two Comisana ewes were divided into 4 groups (8 sheep/group): (1) supplemented with whole flaxseed (FS); (2) supplemented with Ascophyllum nodosum (AG); (3) supplemented with a combination of flaxseed and A. nodosum (FS+AG); and (4) control (C; no supplementation). On d 22 of the experiment, cortisol concentrations in sheep blood were measured after an injection of ACTH. Cellular immune response was evaluated by intradermic injection of phytohemagglutinin (PHA) at 0, 15, and 30. d of the trial. Humoral response to ovalbumin (OVA) was measured at 0, 15, and 30. d. At 0, 15, and 30. d of the experiment, blood samples were collected from each ewe to determine production of T-helper (Th)1 cytokines (IL-12 and IFN-γ), and Th2 cytokines (IL-10, IL-4, IL-13), and concentrations of heat shock proteins (HSP) 70 and 90. Ewes supplemented with flaxseed alone had greater cortisol concentrations and a longer-lasting cell-mediated immune response compared with ewes in the control and other groups. Anti-OVA IgG concentrations increased in all groups throughout the trial, even though ewes in the FS+AG group had the lowest anti-OVA IgG concentrations at 15. d. The level of IL-10 increased in all groups throughout the experiment; the FS+AG group had the lowest IL-13 concentration at 15 and 30. d. The concentration of HSP 70 increased in AG ewes at the end of the experiment and decreased in FS ewes, whereas that of HSP 90 increased in FS ewes compared with FS+AG ewes. Flaxseed supplementation was found to influence in vivo HPA activation in heat-stressed sheep, resulting in increased cortisol concentrations, probably to meet increased energy demand for thermoregulation. Flaxseed supplementation also supported Th1 response via a complex cross-talk between IL-10, IL-12, and IFN-γ production. © 2014 American Dairy Science Association.


PubMed | Council for Research and Experimentation in Agriculture
Type: Journal Article | Journal: Recent patents on biotechnology | Year: 2012

Penicillium species are a widespread source of biologically active compounds and enzymes which are exploited in biotechnologies. The ongoing discovery of new species, their biochemical and molecular characterization, and the application of the new findings in diverse industrial processes stimulate an increasing interest of patentees worldwide. An overview of the patents released in the last four years in agriculture, bioremediation, and in several industrial fields for the production of biofuels, food, cosmetics and pharmaceuticals is proposed for an exhaustive appreciation of the potential cues offered to inventors by these fungi.

Loading Council for Research and Experimentation in Agriculture collaborators
Loading Council for Research and Experimentation in Agriculture collaborators