Shandong Cotton Research Center

Jinan, China

Shandong Cotton Research Center

Jinan, China
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Zhao L.,Shandong Normal University | Ding Q.,Peking University | Zeng J.,Peking University | Wang F.-R.,Shandong Cotton Research Center | And 3 more authors.
Phytochemical Analysis | Year: 2012

Introduction Cotton is an important economic crop. Genetic, developmental and molecular studies of cotton require high-quality total RNA from different tissues. Due to the richness in polyphenols and polysaccharides, the Trizol-based methods and other commercial kits are unsuitable for RNA isolation from cotton. Available methods are generally laborious and time-consuming. Objective To develop an easy, simple and rapid cetyltrimethylammonium bromide (CTAB)-ammonium acetate protocol that takes less time and obtains high yield and quality of RNA from polysaccharide- and polyphenol-rich cotton tissues. Methodology Based on the original CTAB protocol, we used phenol-chloroform and chloroform-isoamyl alcohol to remove proteins, polysaccharides and polyphenols, and ammonium acetate to precipitate RNA, reducing the incubation time prior to RNA precipitation. After adding ammonium acetate to precipitate RNA, all centrifugation steps (14000 × g) were carried out at 4°C to avoid degradation. Results The procedure took only 1.5 h and was suitable for different cotton tissues. The A260:A280 ratios ranged from 1.80 to 1.85 with clear 28 s and 18 s ribosomal RNA bands in 1.2% agarose gel. The isolated RNA was usable for downstream molecular studies, such as reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. Conclusion The CTAB-ammonium acetate method is easy, rapid, low-cost and effective for high-quality RNA isolation from polysaccharide- and polyphenol-rich cotton tissues. Copyright © 2012 John Wiley & Sons, Ltd.

Liu R.,Nanjing Agricultural University | Liu R.,Shandong Cotton Research Center | Wang B.,Nanjing Agricultural University | Guo W.,Nanjing Agricultural University | And 4 more authors.
Molecular Breeding | Year: 2012

Quantitative trait loci (QTL) mapping provides a powerful tool for unraveling the genetic basis of yield and yield components as well as heterosis in upland cotton. In this research, a molecular linkage map of Xiangzamian 2 (Gossypium hirsutum L.)-derived recombinant inbred lines (RILs) was reconstructed based on increased expressed sequence tag-simple sequence repeat markers. Both the RILs and immortalized F2s (IF2) developed through intermating between RILs were grown under multiple environments. Yield and yield components including seed-cotton yield, lint yield, bolls/plant, boll weight, lint percentage, seed index, lint index and fruit branch number were measured and their QTL were repeatedly identified across environments by the composite interval mapping (CIM) method. From a total of 111 non-redundant QTL, 23 were detected in both two populations. In the meantime, multi-marker joint analyses showed that 16 of these QTL had significant environmental interaction. QTL for correlated traits tended to be collocated and most of the QTL for seed-cotton yield and lint yield were associated with QTL for at least one yield component, consistent with the results observed in correlation analyses. For many QTL with significant additive effects, positive alleles from CRI12, the inferior parent with lower yield performance, were associated with trait improvement. Trait performance of IF2s and the large number of QTL with positive dominant effects implied that dominance plays an important role in the genetic basis of heterosis in Xiangzamian 2 and that non-additive inheritance is also an important genetic mode for lint percentage in the population. These QTL can provide the bases for marker-assisted breeding programs of upland cotton. © 2011 Springer Science+Business Media B.V.

Liu R.,Nanjing Agricultural University | Liu R.,Shandong Cotton Research Center | Wang B.,Nanjing Agricultural University | Guo W.,Nanjing Agricultural University | And 2 more authors.
Theoretical and Applied Genetics | Year: 2011

cDNA-AFLP techniques have found new applications in recent years. Currently, the methodology is used to establish differential gene expression and construct linkage maps. In the present study, a transcriptome map based on cDNA-AFLP techniques was constructed using an immortalized F 2 (IF 2) population of 171 lines. The lines were derived from intercrosses between 180 recombinant inbred lines (RILs) of the cotton hybrid Xiangzamian 2 (Gossypium. hirsutum L.). A total of 302 transcriptome-derived fragments (TDFs) were mapped onto 26 linkage groups that covered 2,477.06 cM in length with an average distance of 8.23 cM between two markers. Seventy-one QTL for yield and yield component traits were detected by CIM procedures based on four environments, with 13 QTL identified in at least two environments. Some TDFs co-located with yield QTL were subsequently sequenced and analyzed by online homology searches. Potential candidate genes for yield and yield component traits were found to encode proteins involved in DNA replication, transcription, translation, and biosynthesis regulation. Furthermore, genes regulating metabolic processes signal transduction, transport, and structural components of organelles were identified. Correlation analysis between expression patterns of TDFs and trait performance detected six TDFs positively correlated to both yield and yield heterosis: six TDFs positively correlated to yield, and seven TDFs to yield heterosis. These TDFs have potential for cloning the functional genes responsible for each corresponding trait and have future value in marker-assisted selection. © 2011 Springer-Verlag.

Li Y.,Shandong Agricultural University | Wang N.,CAS Wuhan Botanical Garden | Zhao F.,Shandong Cotton Research Center | Song X.,Shandong Agricultural University | And 3 more authors.
Plant Molecular Biology | Year: 2014

Plants are often subjected to iron (Fe)-deficiency stress because of its low solubility. Plants have evolved two distinct strategies to solubilize and transport Fe to acclimate to this abiotic stress condition. Transcriptomic profiling analysis was performed using Illumina digital gene expression to understand the mechanism underlying resistance responses of roots to Fe starvation in maize, an important Strategy II plant. A total of 3,427, 4,069, 4,881, and 2,610 genes had significantly changed expression levels after Fe-deficiency treatments of 1, 2, 4 or 7 days, respectively. Genes involved in 2′-deoxymugineic acid (DMA) synthesis, secretion, and Fe(III)-DMA uptake were significantly induced. Many genes related to plant hormones, protein kinases, and protein phosphatases responded to Fe-deficiency stress, suggesting their regulatory roles in response to the Fe-deficiency stress. Functional annotation clustering analysis, using the Database for Annotation, Visualization and Integrated Discovery, revealed maize root responses to Fe starvation. This resulted in 38 functional annotation clusters: 25 for up-regulated genes, and 13 for down-regulated ones. These included genes encoding enzymes involved in the metabolism of carboxylic acids, isoprenoids and aromatic compounds, transporters, and stress response proteins. Our work provides integrated information for understanding maize response to Fe-deficiency stress. © 2014 Springer Science+Business Media Dordrecht.

Lin J.,Shandong University | Wang H.-Y.,Shandong Cotton Research Center | Wang K.-Y.,Shandong University | Fan W.-D.,Shandong University | And 2 more authors.
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2014

The zebrafish embryos technology and traditional toxicology methods were used to determined the toxicity of fluopicolide to zebrafish embryos, adult fish and larvae. Fluopicolide had lethal capabilities to the three stages of zebrafish with the LC50(48 h) values to larvae and LC50(96 h) to adult fish and juvenile was 0.204 mg/L, 0.286 mg/L and 1.489 mg/L. 0.0596 mg/L or more concentrations fluopicolide have a certain degree of lethal effect to zebrafish embryos, and the embryos which were treated with high concentrations displayed many of the symptoms, such as pericardial cyst, yolk sac does not absorb, less melanin deposition and fish bending. After 14 days exposure with fluopicolide, the adult zebrafishs expressed the following symptoms: weight loss, reduction in fecundity, the amount of effective eggs were reduced; After treated by 0.0298 mg/L fluopicolide, though 144h offspring larval's survival relatively high, but most surviving fish has still emerged as deformity: pericardial edema and abnormal body bending, etc. Fluopicolide residue in water have the potential hazards to the growth and development of each life stage of zebrafish. ©, 2014, Chinese Society for Environmental Sciences. All right reserved.

Shandong Cotton Research Center | Date: 2013-03-16

A method for identifying HB red-corolla germplasm resources of upland cotton by PCR, including: amplifying genomic DNAs of the upland cotton by PCR using a pair of primers; performing gel electrophoresis on amplified products; and determining whether PCR products is derived from upland HB red-corolla cotton based on results of the gel electrophoresis.

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