Kang M.K.,Kangwon National University |
Kim Y.,Kangwon National University |
Gil S.,Kangwon National University |
Lee S.,Kangwon National University |
And 6 more authors.
Macromolecular Research | Year: 2016
Liquid crystal (LC) emulsions composed of multi-lamellar structures were formulated to enhance the percutaneous absorption of retinyl palmitate across skin barriers. The LC emulsions were prepared by hot process emulsification of an aqueous phase and an oil phase containing retinyl palmitate. Percutaneous absorption levels across synthetic membranes were determined by Franz cell diffusion study. The diffusional absorption level of the oil phase in the LC emulsions was also visualized by fluorescence imaging of Nile red-containing emulsions on synthetic membranes. Transport of retinyl palmitate across pig skin was fluorescently visualized. In vitro cell culture study showed that the LC emulsions enhanced cellular metabolism and proliferation of fibroblasts in comparison to the plain emulsion. The multi-lamellar structures composed of aligned fatty alcohol chains were revealed using freezefracture scanning electron microscopy and polarized light microscopy. The LC emulsions showed increased retention at the membrane as well as at the acceptor in comparison to plain oil-in-water emulsions. Total fluorescence intensity at the membrane with the LC emulsions was higher compared to plain emulsions. Confocal laser scanning microscopy (CLSM) of cryo-cut cross-sections revealed that Nile red was highly localized in the epidermis layers, particularly in the stratum corneum in comparison to the plain emulsions. LC emulsions also enhanced transport of retinyl palmitate at cellular levels, in addition to facilitating percutaneous absorption across dermal layers. [Figure not available: see fulltext.] © 2016, The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht.
PubMed | Cosmax nter and Kangwon National University
Type: | Journal: International journal of pharmaceutics | Year: 2017
An EGF fragment and doxorubicin were chemically co-decorated on single magnetic nanoparticles for concomitant cancer targeting and treatment. Magnetic nanoparticles (MNPs) were prepared by the precipitation of ferric chloride hydrates in an ammonia solution and subsequent surface-functionalization with amines. The terminal thiol group of the EGF fragment (EGFfr) was first conjugated to surface amines of the MNPs using a heterofunctional crosslinker, and doxorubicin was sequentially conjugated to the MNPs via a hydrazone linker, where the degree of substation of the surface amines to EGF was varied from 1% to 40%. The decorated doxorubicin showed clear pH-dependency in the release profile, and doxorubicin showed fast release at pH 5.0 in comparison to pH 7.4. The EGF-decorated MNPs were tested for differential cellular uptakes against EGF overexpressing cells (A549), and the uptake levels were gradually increased to 10%; however, they became saturated above 10%, which was quantified by ICP-OES. Internalized doxorubicin was also visualized by confocal microscopy, and A549 cells with EGF-decorated MNPs with EGF decoration showed higher fluorescence intensity of doxorubicin than those with non-decorated MNPs. The anti-cancer activity of the MNPs was compared at various concentrations of doxorubicin and EGFfr in MNPs. Decoration of EGFfr significantly increased the anti-cancer activity of doxorubicin-incorporated MNPs in A549 cells; however, EGFfr alone did not affect the viability of the cells. Thus, we concluded that MNPs with optimized EGFfr and doxorubicin ratios showed higher targeting and drug payload against EGF receptor overexpressing cancer cells.
PubMed | Cosmax nter
Type: Journal Article | Journal: International journal of cosmetic science | Year: 2015
The purpose of this study was whether P/M or T/M inhibits IR-induced decrease of collagens in human dermal fibroblasts, using P/M or T/M blocked near-IR (NIR) transmittance significantly in spectrophotometer measurement.As metal oxides are effective inorganic molecules for intercepting IR radiation, we have developed metal oxide-coating PMMA (P/M) or Talc (T/M) as a new IR blocker. Inhibitory effect of the new IR blocker on collagen degradation was measured by gene and protein expressions of procollagens and MMPs, respectively, in IR-irradiated Hs68 cell line.Using P/M or T/M inhibited IR-induced increases of MMP-1, MMP-3 and MMP-9, and IR-induced decreases of type 1 and 4 procollagen in a dose-dependent manner in dermal fibroblasts. In addition, using both P/M and T/M blocked the increase of cell media temperature induced by IR lamp.The results suggest that P/M or T/M can inhibit decrease of collagens by blocking IR-induced heat transmission in human dermal fibroblasts.