Corporacion para Investigaciones Biologicas CIB

Medellín, Colombia

Corporacion para Investigaciones Biologicas CIB

Medellín, Colombia
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Turissini D.A.,University of North Carolina at Chapel Hill | Gomez O.M.,Corporacion para Investigaciones Biologicas CIB | Gomez O.M.,University of Antioquia | Teixeira M.M.,Northern Arizona Center for Valley Fever Research | And 3 more authors.
Fungal Genetics and Biology | Year: 2017

The use of molecular taxonomy for identifying recently diverged species has transformed the study of speciation in fungi. The pathogenic fungus Paracoccidioides spp has been hypothesized to be composed of five phylogenetic species, four of which compose the brasiliensis species complex. Nuclear gene genealogies support this divergence scenario, but mitochondrial loci do not; while all species from the brasiliensis complex are differentiated at nuclear coding loci, they are not at mitochondrial loci. We addressed the source of this incongruity using 11 previously published gene fragments, 10 newly-sequenced nuclear non-coding loci, and 10 microsatellites. We hypothesized and further demonstrated that the mito-nuclear incongruence in the brasiliensis species complex results from interspecific hybridization and mitochondrial introgression, a common phenomenon in eukaryotes. Additional population genetic analyses revealed possible nuclear introgression but much less than that seen in the mitochondrion. Our results are consistent with a divergence scenario of secondary contact and subsequent mitochondrial introgression despite the continued persistence of species boundaries. We also suggest that yeast morphology slightly—but significantly—differs across all five Paracoccidioides species and propose to elevate four of these phylogenetic species to formally described taxonomic species. © 2017 The Authors


PubMed | Pontifical Bolivarian University, Colombia Hospital La Maria, Corporacion para Investigaciones Biologicas CIB and University of Antioquia
Type: Case Reports | Journal: Medical mycology | Year: 2015

Coccidioides immitis and C. posadasii are the etiologic agents of coccidioidomycosis, an endemic fungal disease of the Americas. In Colombia, this mycosis is uncommon, and only five cases, two of them imported, have been documented.By means of DNA sequencing, C. immitis was identified in formalin-fixed, paraffin-embedded archival tissues samples from the 5th Colombian patient diagnosed in 1997. The patient was born in Pinto, Department of Magdalena, and had never visited other geographic regions, a reason to consider that the mycosis had been acquired locally.This species is primarily found in California although it has been occasionally reported in other geographic areas such as Mexico and Brazil. This is the first indigenous report of C. immitis-associated coccidioidomycosis in a Colombian patient.


Garcia S.D.G.,Corporacion para Investigaciones Biologicas CIB | Garcia S.D.G.,University of Antioquia | Lorza A.R.,University of Tolima | Pelaez C.A.,University of Antioquia
Summa Phytopathologica | Year: 2014

Microorganisms for biological control are capable of producing active compounds that inhibit the development of phytopathogens, constituting a promising tool to obtain active principles that could replace synthetic pesticides. This study evaluated the ability of several potential biocontrol microorganisms to produce active extracellular metabolites. In vitro antagonistic capability of 50 bacterial isolates from rhizospheric soils of “criolla” potato (Solanum phureja) was tested through dual culture in this plant with different plant pathogenic fungi and bacteria. Isolates that showed signifcantly higher antagonistic activity were fermented in liquid media and crude extracts from the supernatants had their biological activities assessed by optical density techniques. Inhibitory effect on tested pathogens was observed for concentrations between 0.5% and 1% of crude extracts. There was a correlation between the antimicrobial activity of extracts and the use of nutrient-rich media in bacteria fermentation. Using a bioguided method, a peptidic compound, active against Fusarium oxysporum, was obtained from the 7ANT04 strain (Pyrobaculum sp.). Analysis by nuclear magnetic resonance and liquid chromatography coupled to mass detector evidenced an 11-amino acid compound. Bioinformatic software using raw mass data confrmed the presence of a cyclic peptide conformed by 11 mostly non-standard amino acids. © 2014, Universidade Estadual Paulista (UNESP). All rights reserved.


Canteros C.E.,Instituto Nacional Of Enfermedades Infecciosas Inei Anlis Dr Carlos G Malbran | Velez H. A.,Hospital Pablo Tobon Uribe | Velez H. A.,Laboratorios Dinamica | Velez H. A.,Pontifical Bolivarian University | And 7 more authors.
Medical Mycology | Year: 2015

Coccidioides immitis and C. posadasii are the etiologic agents of coccidioidomycosis, an endemic fungal disease of the Americas. In Colombia, this mycosis is uncommon, and only five cases, two of them imported, have been documented. By means of DNA sequencing, C. immitis was identified in formalin-fixed, paraffin-embedded archival tissues samples from the 5th Colombian patient diagnosed in 1997. The patient was born in Pinto, Department of Magdalena, and had never visited other geographic regions, a reason to consider that the mycosis had been acquired locally. This species is primarily found in California although it has been occasionally reported in other geographic areas such as Mexico and Brazil. This is the first indigenous report of C. immitis-associated coccidioidomycosis in a Colombian patient. © The Author 2015. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved.


PubMed | Fungal Biodiversity Center, National Autonomous University of Mexico, Oswaldo Cruz Foundation, Corporacion para Investigaciones Biologicas CIB and 4 more.
Type: Journal Article | Journal: PLoS neglected tropical diseases | Year: 2016

Histoplasma capsulatum comprises a worldwide complex of saprobiotic fungi mainly found in nitrogen/phosphate (often bird guano) enriched soils. The microconidia of Histoplasma species may be inhaled by mammalian hosts, and is followed by a rapid conversion to yeast that can persist in host tissues causing histoplasmosis, a deep pulmonary/systemic mycosis. Histoplasma capsulatum sensu lato is a complex of at least eight clades geographically distributed as follows: Australia, Netherlands, Eurasia, North American classes 1 and 2 (NAm 1 and NAm 2), Latin American groups A and B (LAm A and LAm B) and Africa. With the exception of the Eurasian cluster, those clades are considered phylogenetic species.Increased Histoplasma sampling (n = 234) resulted in the revision of the phylogenetic distribution and population structure using 1,563 aligned nucleotides from four protein-coding regions. The LAm B clade appears to be divided into at least two highly supported clades, which are geographically restricted to either Colombia/Argentina or Brazil respectively. Moreover, a complex population genetic structure was identified within LAm A clade supporting multiple monophylogenetic species, which could be driven by rapid host or environmental adaptation (~0.5 MYA). We found two divergent clades, which include Latin American isolates (newly named as LAm A1 and LAm A2), harboring a cryptic cluster in association with bats.At least six new phylogenetic species are proposed in the Histoplasma species complex supported by different phylogenetic and population genetics methods, comprising LAm A1, LAm A2, LAm B1, LAm B2, RJ and BAC-1 phylogenetic species. The genetic isolation of Histoplasma could be a result of differential dispersion potential of naturally infected bats and other mammals. In addition, the present study guides isolate selection for future population genomics and genome wide association studies in this important pathogen complex.


Robledo J.A.,Corporacion Para Investigaciones Biologicas CIB | Robledo J.A.,Pontifical Bolivarian University | Murillo A.M.,Corporacion Para Investigaciones Biologicas CIB | Rouzaud F.,Corporacion Para Investigaciones Biologicas CIB
IUBMB Life | Year: 2011

The production of pigments by bacterial colonies has sparked interest among bacteriologists since the 19th century, whether for taxonomy or, in the case of carotenoids for their association with antibiotics resistance. Mycobacteria have gained a very special place in the bacterial world due to their clinical importance. Alone, Mycobacterium tuberculosis is responsible for about two million deaths annually worldwide making tuberculosis one of the most influential diseases in the history of mankind. Almost half of the Nontuberculous Mycobacteria species identified are associated with opportunistic infections in animals and humans. Mycobacterial pigmentary characteristics started to be documented about 80 years ago; but to date, their main use has been only for limited taxonomic and identification purposes. While mycobacterial pigments, especially carotenoids have been clearly associated with cellular photoprotection and survival, the regulation of their production and their physiological role have been largely unstudied. Recent advances in deciphering mycobacterial genomes and characterization of carotenoid synthesis genes, combined with an urgent need for innovative approaches to understand Mycobacterium tuberculosis pathogenic properties open new avenues for exciting research opportunities that might lead to new therapeutic strategies against a devastating secular disease. © 2011 IUBMB.


Benavides A.M.,Corporacion Para Investigaciones Biologicas CIB | Wolf J.H.D.,University of Amsterdam | Duivenvoorden J.F.,University of Amsterdam
Journal of Tropical Ecology | Year: 2013

The contribution of vegetative recruitment by non-tree species to the regeneration of tropical forests in man-made clearings or tree-fall gaps tends to be ignored. In a series of field studies near Amacayacu, Colombian Amazonia, we tested if hemiepiphytic aroids quickly colonize such open habitats through seed dispersal, sprouting plant fragments, or lateral invasion of flagellar aroids from the closed forest nearby. A seed germination experiment applying two soil substrates and three shade levels showed that abundant light reduced the germination success of three Philodendron species. A total of 400 cuttings from five Philodendron species were placed in forest clearings and almost 12% of these sprouted within 14 wk. Monitoring more than 2000 aroid plants over 14 mo in different habitats showed that recruitment was low (0.3 plants per 10 m 2) compared with initial densities (3.1 plants per 10 m2). Flagellar aroids grew about 2.5 times faster than non-flagellar aroids. In forest edges they reached a mean apical growth of 98 cm in 14 mo. However, non-flagellar aroids were five to six times more abundant than flagellar individuals everywhere. It was concluded that hemiepiphytic aroids colonize open habitats mostly through a post-disturbance survival of plants or plant fragments. Copyright © 2013 Cambridge University Press.


PubMed | Pontifical Bolivarian University and Corporacion para Investigaciones Biologicas CIB
Type: Evaluation Studies | Journal: Revista de salud publica (Bogota, Colombia) | Year: 2014

Using cost-benefit analysis for comparing the thin-layer agar culture method to the standard multiple proportion method used in diagnosing multidrug-resistant tuberculosis (MDR TB).A cost-benefit evaluation of two diagnostic tests was made at the Corporacin para Investigaciones Biolgicas (CIB) in Medelln, Colombia.100 patients were evaluated; 10.8% rifampicin resistance and 14.3% isoniazid resistance were found. A computer-based decision tree model was used for cost-effectiveness analysis (Treeage Pro); the thin-layer agar culture method was most cost-effective, having 100% sensitivity, specificity and predictive values for detecting rifampicin and isoniazid resistance. The multiple proportion method value was calculated as being US$ 71 having an average 49 day report time compared to US$ 18 and 14 days for the thin-layer agar culture method.New technologies have been developed for diagnosing tuberculosis which are apparently faster and more effective; their operating characteristics must be evaluated as must their effectiveness in terms of cost-benefit. The present study established that using thin-layer agar culture was cheaper, equally effective and could provide results more quickly than the traditional method. This implies that a patient could receive MDR TB treatment more quickly.


PubMed | Pontifical Bolivarian University, National University of Colombia and Corporacion para Investigaciones Biologicas CIB
Type: Journal Article | Journal: Genome announcements | Year: 2016

Mycobacterium africanum is a member of the Mycobacteriumtuberculosis complex. Most commonly found in West African countries, it has scarcely been described in South America. Here, we report the first genome sequence of a Colombian M.africanum clinical isolate. It is composed of 4,493,502bp, with 4,069 genes.


PubMed | Pontifical Bolivarian University, J. Craig Venter Institute and Corporacion para Investigaciones Biologicas CIB
Type: Journal Article | Journal: Genome announcements | Year: 2016

Colombia is one of the 105 countries that has reported at least one case of extensively drug-resistant tuberculosis (XDR-TB). The Mycobacterium tuberculosis Haarlem genotype is ubiquitous worldwide. Here, we report the high-quality draft genome sequence of a Colombian Haarlem XDR-TB clinical isolate composed of 4,329,127bp with 4,386 genes.

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