Blair M.W.,National University of Colombia |
Blair M.W.,Tennessee State University |
Cordoba J.M.,CORPOICA |
Munoz C.,Generation Challenge Program |
Yuyo D.K.,National University of Colombia
PLoS ONE | Year: 2014
Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called ''singleton''BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single dior tri-nucleotide motifs that were likely to be polymorphic (ATT or AG motifs, principally) and useful for primer design and individual marker mapping. When these novel SSR markers were evaluated in the DOR364×G19833 population parents, 136 markers revealed polymorphism and 106 were mapped. Genetic mapping resulted in a map length of 2291 cM with an average distance between markers of 5.2 cM. The new genetic map was compared to the most recent cytogenetic analysis of common bean chromosomes. We found that the new singleton BES-SSR were helpful in filling peri-centromeric spaces on the cytogenetic map. Short genetic distances between some new singleton-derived BES-SSR markers was common showing suppressed recombination in these regions compared to other parts of the genome. The correlation of singleton-derived SSR marker distribution with other cytogenetic features of the bean genome is discussed. © 2014 Blair et al.
Rueda A.,University of Antioquia |
Roman Y.,University of Antioquia |
Lobo M.,CORPOICA |
Pelaez C.,University of Antioquia
Tropical Plant Pathology | Year: 2011
Plant defense responses to stress have attracted interest because of their similarity to reported mammalian stress responses. To investigate plant responses to abiotic stress, caspase 10-like enzymatic activities of Lycopersicon hirsutum and Lycopersicon esculentum were assayed in response to copper chloride and paraquat induction. Caspase 10-like activity was greatest at 40 mM CuCl 2 at 9 h after elicitation in both L. hirsutum and L. esculentum, for which respective response slopes (Δ absorbance per Δ minute) were 0.0054 and 0.0022. The response for L. hirsutum was less variable and significantly greater than for L. esculentum. Elicitation of caspase 10-like activity by paraquat was greatest at around 2 h after treatment in both species with slopes of 0.013 and 0.0012, respectively for L. hirsutum and L. esculentum. The direct determination of caspase 10-like activity in tomato seedlings treated with copper and paraquat, using a specific substrate for mammals, suggested that the observed responses were related to apoptosis processes under abiotic induction. © By the Brazilian Phytopathological Society.
Prain G.,International Potato Center |
Gonzales N.,International Potato Center |
Arce B.,CORPOICA |
Tenorio J.,Agrarian National University
Acta Horticulturae | Year: 2010
Strategies and methods have been developed in Lima, Peru to enable urban agricultural producers to take fuller advantage of the nearby location of a wide range of high quality fresh and processed food markets. Horticultural producers in the poor eastern fringe of Lima are currently constrained by a lack of technical and entrepreneurial skills and the capacity for jointly identifying and meeting demand. Through building a collaborative research and development platform among local producers, the watershed irrigation committee and the District Municipality staff, Urban Harvest has implemented three main research and development interventions to make local horticulture more sustainable and profitable, (1) implementation of "Farmer Field Schools" to stimulate innovation and learning in ecological production in an urban setting, (2) design and implementation of a "School for Urban Farmers" to strengthen and empower the producer organizations and establish new marketing opportunities, and (3) the integration of agriculture within local government administration to enhance municipal support for safe and healthy horticultural production and marketing.
Effects of supplementation with oregano essential oil on ileal digestibility, intestinal histomorphology, and performance of broiler chickens [Efectos de la suplementación con aceites esenciales de orégano sobre la digestibilidad ileal, histomorfometría intestinal y comportamiento productivo de pollos de engorde]
Betancourt L.L.,National University of Colombia |
Betancourt L.L.,University of la Salle of Colombia |
Ariza C.J.,CORPOICA |
Afanador G.,National University of Colombia
Revista Colombiana de Ciencias Pecuarias | Year: 2012
Objective: evaluate the effects of different chemiotypes of oregano essential oil (OEO) on protein, fat, and energy ileal digestibility of broiler chickens at 21 days of age. Methods: six treatments were evaluated: 200 ppm of OEO from three varieties produced and ground in Sabana de Bogotá-Colombia: O. vulgare L ssp hirtum (OH); O. vulgare L. (OL) y O. majorana (OM); 500 ppm Chlortetracycline (AB) and a control without additives (C). Between 14 and 21 days of age the chickens were fed with starter diets supplemented with 0.5 g/kg chromium oxide (Cr2O3) as a inert marker. The ileal digesta was collected, and protein, fat, energy, and chromium were analyzed in both feed and ileal content, and ID was calculated. Results: AB group showed a higher protein ID compared to control group, 83.7 and 75.3%, respectively (p<0.05). Both OM and AB experimental groups presented higher values of ID for energy and fat compared to control group, 92.3, 91.7 and 84.2%, respectively (p<0.05). These groups also presented a higher body weight at day 21 (p<0.05). However, the difference disappeared at 42 d of age. A negative correlation was estimated between body weight and carvacrol intake (r: -0.55), but it was positive with thymol intake (r:0.46, p<0.05). Conclusions: the results showed different responses of chickens depending on the composition of OEO.
Severino L.S.,Embrapa Algodao |
Severino L.S.,Texas Tech University |
Cordoba G O.J.,CORPOICA |
Zanotto M.D.,Sao Paulo State University |
Auld D.L.,Embrapa Algodao
Seed Science and Technology | Year: 2012
The caruncle is a structure present in the micropylar region of Euphorbiaceae seeds. This structure has the ecological function of promoting seed dispersal by ants (myrmecochory), but it is debated whether it also has an agronomical importance influencing seed germination. The influence of the caruncle on castor (Ricinus communis) seed germination was evaluated under low soil water content and high soil salinity. Seeds were germinated at soil water storage capacities varying from 22 to 50% and salinities (NaCl) varying from 0 to 10 dS m-1. The germination (%) increased following the increments in soil moisture, but the caruncle had no influence on this process at any moisture level. In one genotype, more root dry mass was produced when caruncle was excised. Increasing salinity reduced the percentage and speed of germination of castor seeds, but no influence of caruncle was detected. No evidence of caruncle influencing castor seed germination was found under low soil water content and high salinity.