CryoCenter Cord Blood Bank

Moscow, Russia

CryoCenter Cord Blood Bank

Moscow, Russia
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Romanov Y.A.,Russian Cardiology Research and Production Complex | Balashova E.E.,CryoCenter Cord Blood Bank | Kabaeva N.V.,Russian Cardiology Research and Production Complex | Dugina T.N.,CryoCenter Cord Blood Bank
Bulletin of Experimental Biology and Medicine | Year: 2017

We studied the expression of different classes of surface molecules (CD13, CD29, CD40, CD44, CD54, CD71, CD73, CD80, CD86, CD90, CD105, CD106, CD146, HLA-I, and HLA-DR) in mesenchymal stromal cells from human umbilical cord and bone marrow during co-culturing with nucleated umbilical cord blood cells. Expression of the majority of surface markers in both types of mesenchymal stromal cells was stable and did not depend on the presence of the blood cells. Significant differences were found only for cell adhesion molecules CD54 (ICAM-1) and CD106 (VCAM-1) responsible for direct cell—cell contacts with leukocytes and only for bone marrow derived cells. © 2017, Springer Science+Business Media New York.


Romanov Y.A.,Russian Cardiology Research and Production Complex | Balashova E.E.,Russian Cardiology Research and Production Complex | Kabaeva N.V.,Russian Cardiology Research and Production Complex | Dugina T.N.,CryoCenter Cord Blood Bank
Bulletin of Experimental Biology and Medicine | Year: 2017

Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes. © 2017, Springer Science+Business Media New York.

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