Ramirez-Suarez J.C.,Coordinacion Of Tecnologia Of Alimentos Of Origen Animal Research Center En Alimentacion sarrollo |
Pacheco-Aguilar R.,Coordinacion Of Tecnologia Of Alimentos Of Origen Animal Research Center En Alimentacion sarrollo |
Scheuren-Acevedo S.M.,Coordinacion Of Tecnologia Of Alimentos Of Origen Animal Research Center En Alimentacion sarrollo |
Garcia-Sanchez G.,Coordinacion Of Tecnologia Of Alimentos Of Origen Animal Research Center En Alimentacion sarrollo |
And 4 more authors.
Journal of Food Safety | Year: 2015
This study aimed to evaluate the effect of a natural antimicrobial agent (Pronat) on selected quality indicators during the shelf life of vacuum-packed frankfurters made from jumbo squid (Dosidicus gigas) muscle. Frankfurters with and without Pronat (0.1%) were vacuum-packed and stored at 2-4C for up to 28 days. The physicochemical parameters (water loss, pH, thiobarbituric acid value, peroxide value, moisture, texture [texture profile analysis and sharing force], folding test performance, water-holding capacity [WHC], drip loss and color) as well as the microbial counts (total aerobic and anaerobic count, total and fecal coliform) of the samples were assessed. The samples with Pronat were physicochemically stable and in good microbiological condition up to 21 days compared with the control. The main changes were attributed to color, WHC and drip loss. The Pronat concentration used in squid frankfurters greatly improved their microbiological shelf life but did not considerably improve the physicochemical quality indicators assessed in the present study. © 2015 Wiley Periodicals, Inc.
PubMed | Coordinacion Of Tecnologia Of Alimentos Of Origen Animal Research Center En Alimentacion sarrollo, Polytechnic University of Sinaloa and Programa Of Investigacion En Biotecnologia Instituto Nacional Of Investigaciones Forestales
Type: | Journal: Applied biochemistry and biotechnology | Year: 2016
Plant proteases are capable of performing several functions in biological systems, and their use is attractive for biotechnological process due to their interesting catalytic properties. Bromelia pinguin (aguama) is a wild abundant natural resource in several regions of Central America and the Caribbean Islands but is underutilized. Their fruits are rich in proteases with properties that are still unknown, but they represent an attractive source of enzymes for biotechnological applications. Thus, the proteolytic activity in enzymatic crude extracts (CEs) from wild B. pinguin fruits was partially characterized. Enzymes in CEs showed high proteolytic activity at acid (pH 2.0-4.0) and neutral alkaline (pH 7.0-9.0) conditions, indicating that different types of active proteases are present. Proteolytic activity inhibition by the use of specific protease inhibitors indicated that aspartic, cysteine, and serine proteases are the main types of proteases present in CEs. Activity at pH 3.0 was stable in a broad range of temperatures (25-50C) and retained its activity in the presence of surfactants (SDS, Tween-80), reducing agents (DTT, 2-mercapoethanol), and organic solvents (methanol, ethanol, acetone, 2-propanol), which suggests that B. pinguin proteases are potential candidates for their application in brewing, detergent, and pharmaceutical industries.