San Nicolás de los Garza, Mexico
San Nicolás de los Garza, Mexico

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Duarte-Sierra A.,Research Center en Alimentacion y Desarrollo | Aispuro-Hernandez E.,Research Center en Alimentacion y Desarrollo | Vargas-Arispuro I.,Coordinacion de Ciencia de los Alimentos | Islas-Osuna M.A.,Research Center en Alimentacion y Desarrollo | And 2 more authors.
Journal of the Science of Food and Agriculture | Year: 2016

Background: Gaseous fumigants are commonly employed to control fungal decay of cold-stored grapes. So far it is not clear if these fumigants, besides the direct interaction against fungal structures, induce transcriptional responses of defensive markers. In order to contribute to understanding the mechanisms by which these fumigants exert their effect, we studied the influence of ozone (O3) and sulfur dioxide (SO2) on the decay caused by Botrytis cinerea, and the quality and expression of the defense-related genes chitinase, β-1,3-glucanase and phenylalanine ammonia-lyase (PAL) in the table grape cultivars 'Redglobe' and 'Sugraone'. Results: The application of SO2 or O3 delayed decay of both table grape cultivars caused by B. cinerea compared with the inoculated control. O3 treatments altered weight loss, firmness and shatter in both cultivars. Significant upregulation of chitinase and β-1,3-glucanase were observed in SO2-treated 'Redglobe' berries stored at 2°C. O3 treatment transiently increased the expression of chitinase and PAL in 'Redglobe' and 'Sugraone' berries, respectively. Conclusion: Ozone and sulfur dioxide treatments can influence the expression patterns of PAL, chitinase and β-1,3-glucanase to different extents in different grape cultivars and under different exposure conditions. The upregulation of these genes may be involved in the mechanism by which these fumigants inhibit the decay caused by pathogenic fungi. © 2016 Society of Chemical Industry.


Mendoza-Wilson A.M.,Coordinacion de Tecnologia de Alimentos de Origen Vegetal | Armenta-Vazquez M.E.,Institutotecnologico Of Los Mochis | Castro-Arredondo S.I.,Coordinacion de Tecnologia de Alimentos de Origen Vegetal | Espinosa-Plascencia A.,Coordinacion de Ciencia de Los Alimentos | And 4 more authors.
Journal of Molecular Structure | Year: 2013

The apple peel, which is an agro-industrial byproduct, has a high content of natural antioxidants and could be a source of functional ingredients for easily oxidizable foods, as well as a source of nutraceuticals for the treatment of diseases of oxidative origin. The ability to inhibit free radicals of an apple peel aqueous extract (APAE) and its fraction of procyanidins (P) was investigated, based on both the second-order rate constants (Ks) and inhibition of lipid peroxidation (ILP). For this purpose, ultraviolet-visible spectrophotometry (UV-Vis) adapted to stopped-flow, and computational methodologies as Density Functional Theory (DFT) were used. It was found that the P fraction was the one that showed the highest Ks in aqueous medium, while the largest percentage of ILP in emulsion was accomplished by the APAE. The compounds which could confer the high antiradical activity to apple peel are mainly quercetin-3-galactoside, quercetin-3- glucoside, procyanidin B-2 and cyanidin-3-glucoside. © 2012 Elsevier B.V. All rights reserved.


Mendoza-Wilson A.M.,Coordinacion de Tecnologia de Alimentos de Origen Vegetal | Castro-Arredondo S.I.,Coordinacion de Tecnologia de Alimentos de Origen Vegetal | Espinosa-Plascencia A.,Coordinacion de Ciencia de Los Alimentos | Del Refugio Robles-Burgueno M.,Coordinacion de Ciencia de Los Alimentos | And 2 more authors.
Heliyon | Year: 2016

The apple is a food rich in diverse classes of polyphenols (PP), among which the proanthocyanidins (PCs), which are primarily concentrated in the skin, are one of the most abundant. These compounds are of considerable interest for their possible positive health effects because of their antioxidant properties. However, depending on the classes of PP present (chemical composition) and their relative concentrations in the apple skin, their antioxidant effects vary and some of their components can even generate prooxidant effects. This work determined the chemical composition and antioxidant-prooxidant potential of a polyphenolic extract (PPE) and a proanthocyanidin-rich fraction (PRF) of apple skin, along with the contribution of their most abundant individual compounds, based on their copper chelating ability, ease in reducing peroxidase-generated free radicals and TEAC (Trolox-Equivalent Antioxidant Capacity) assay. For this purpose, chromatographic and colorimetric methods were used. The majority compounds identified in PPE were flavan-3-ols (44.58%), flavonols (42.89%) and dihydrochalcones (11.60%). In PRF, we detected monomers and oligomers from dimers to heptamers, which were composed of 97% (-)-epicatechin and 3% (+)-catechin. The antioxidant potential was notably higher in PRF than in PPE. The (-)-epicatechin monomer and the procyanidin B2 dimer showed more ease in reducing peroxidase-generated free radicals compared to other compounds of the apple skin, whereas phloridzin dihydrochalcone produced prooxidant effects. © 2016 The Authors.


Mendoza-Wilson A.M.,Coordinacion de Tecnologia de Alimentos de Origen Vegetal | Carmelo-Luna F.J.,Coordinacion de Tecnologia de Alimentos de Origen Vegetal | Astiazaran-Garcia H.,Coordinacion de Nutricion | Mata-Haro V.,Coordinacion de Ciencia de los Alimentos | And 3 more authors.
Journal of Functional Foods | Year: 2016

The absorption of pure procyanidins (PP) and procyanidins from a procyanidin-rich fraction of apple skin (PRFAS) was determined, employing the IEC-18 cellular line as a model of paracellular transport. The percentages of absorption for each compound from the PRFAS were: (−)-epicatechin (0.17%), procyanidin B2 (0.13%), procyanidin C1 (0.1%), tetramer 3 (0.05%), tetramer 2 (0.04%), (+)-catechin (0.009%), procyanidin B1 (0.007%) and tetramer 1 (0.002%). The percentages of absorption of PP were significantly larger: 5.52, 4.25 and 1.10% for procyanidins B2, B1 and C1, respectively. Together, the concentration, degree of polymerization and structure determine the cellular absorption of each procyanidin. © 2016 Elsevier Ltd


PubMed | Coordinacion de Ciencia de los Alimentos and Research Center en Alimentacion y Desarrollo
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

Gaseous fumigants are commonly employed to control fungal decay of cold-stored grapes. So far it is not clear if these fumigants, besides the direct interaction against fungal structures, induce transcriptional responses of defensive markers. In order to contribute to understanding the mechanisms by which these fumigants exert their effect, we studied the influence of ozone (O3) and sulfur dioxide (SO2) on the decay caused by Botrytis cinerea, and the quality and expression of the defense-related genes chitinase, -1,3-glucanase and phenylalanine ammonia-lyase (PAL) in the table grape cultivars Redglobe and Sugraone.The application of SO2 or O3 delayed decay of both table grape cultivars caused by B. cinerea compared with the inoculated control. O3 treatments altered weight loss, firmness and shatter in both cultivars. Significant upregulation of chitinase and -1,3-glucanase were observed in SO2 -treated Redglobe berries stored at 2 C. O3 treatment transiently increased the expression of chitinase and PAL in Redglobe and Sugraone berries, respectively.Ozone and sulfur dioxide treatments can influence the expression patterns of PAL, chitinase and -1,3-glucanase to different extents in different grape cultivars and under different exposure conditions. The upregulation of these genes may be involved in the mechanism by which these fumigants inhibit the decay caused by pathogenic fungi.


Mata-Haro V.,Coordinacion de Ciencia de los Alimentos | Resendiz-Sandoval M.,Research Center en Alimentacion y Desarrollo | Hernandez J.,Research Center en Alimentacion y Desarrollo
Food and Agricultural Immunology | Year: 2014

The purpose of this study was to evaluate the extent of cytokine induction and T cell proliferation in lactic acid bacteria (LAB)-stimulated porcine peripheral blood mononuclear cells (PBMCs). Porcine PBMCs were stimulated with phytohaemagglutinin (PHA) and then incubated with Bifidobacterium angulatum, Enterococcus faecalis and Lactobacillus reuteri. A decrease in the mRNA expression of interferon-gamma (IFN-γ) was detected; however, only a significant increase (P<0.05) in interleukin (IL)-10 expression was found in cells stimulated with E. faecalis. No changes in transforming growth factor-beta (TGF-β) were observed with neither of the probiotics tested nor in T cell proliferation between LAB-stimulated and control groups. These results suggest a possible immunomodulatory probiotic effect and this effect could be due to a decrease in the inflammatory T helper 1 (Th1) immune response, and an increase of T regulatory function, as suggested by the augmented expression of IL-10. © 2013 Taylor & Francis.

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