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Contreras-Soto R.I.,State University of Maringá | Mora F.,University of Talca | De Oliveira M.A.R.,COODETEC | Higashi W.,COODETEC | And 2 more authors.
PLoS ONE | Year: 2017

Mapping quantitative trait loci through the use of linkage disequilibrium (LD) in populations of unrelated individuals provides a valuable approach for dissecting the genetic basis of complex traits in soybean (Glycine max). The haplotype-based genome-wide association study (GWAS) has now been proposed as a complementary approach to intensify benefits from LD, which enable to assess the genetic determinants of agronomic traits. In this study a GWAS was undertaken to identify genomic regions that control 100-seed weight (SW), plant height (PH) and seed yield (SY) in a soybean association mapping panel using single nucleotide polymorphism (SNP) markers and haplotype information. The soybean cultivars (N = 169) were field-evaluated across four locations of southern Brazil. The genome-wide haplotype association analysis (941 haplotypes) identified eleven, seventeen and fifty-nine SNP-based haplotypes significantly associated with SY, SW and PH, respectively. Although most marker-Trait associations were environment and trait specific, stable haplotype associations were identified for SY and SW across environments (i.e., haplotypes Gm12 Hap12). The haplotype block 42 on Chr19 (Gm19 Hap42) was confirmed to be associated with PH in two environments. These findings enable us to refine the breeding strategy for tropical soybean, which confirm that haplotype-based GWAS can provide new insights on the genetic determinants that are not captured by the single-marker approach. © 2017 Contreras-Soto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Dranski J.A.L.,West Parana State University | De Matos Malavasi M.,Producao e Comercializacao Agricola Ltda | Malavasi U.C.,Producao e Comercializacao Agricola Ltda | Schuster I.,Producao e Comercializacao Agricola Ltda | Lazaretti N.S.,COODETEC
Semina:Ciencias Agrarias | Year: 2017

Respiratory activity is one of the first biological manifestations of vigor loss and detecting it can help the monitoring and quality control of seed production by seed industries. This research aimed to develop and validate the methodology of the carbon dioxide concentration in the evaluation of physiological quality of Zea mays (L.) seeds. The proposed method is grounded on the measurement of the CO2 content by infrared through a drag system and exhaustion of the gas released by the seed maintained in a closed system up to the detection chamber of the gas meter. Samples of 15, 20 and 25 seeds of 6 lots of cultivar CD 384 Hx were incubated at temperatures of 15, 25 and 40 °C. The CO2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. In parallel, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients among tests were calculated. After determining the reading conditions, the accuracy and precision of the proposed method were evaluated, using 15 seed lots. For the evaluation of respiratory activity in Z. mays seeds, we recommend a sample of 25 seeds, incubated at 15 °C for a maximum of 12 h, which allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO2 as it externalizes precision between successive measurements and agreement to the reference method.

Da Hora Junior B.T.,State University of Santa Cruz | Poloni J.D.F.,University of Caxias do Sul | Poloni J.D.F.,Federal University of Rio Grande do Sul | Lopes M.A.,State University of Santa Cruz | And 11 more authors.
Molecular BioSystems | Year: 2012

This study reports on expression analysis associated with molecular systems biology of cacao-Moniliophthora perniciosa interaction. Gene expression data were obtained for two cacao genotypes (TSH1188, resistant; Catongo, susceptible) challenged or not with the fungus M. perniciosa and collected at three time points through disease. Using expression analysis, we identified 154 and 227 genes that are differentially expressed in TSH1188 and Catongo, respectively. The expression of some of these genes was confirmed by RT-qPCR. Physical protein-protein interaction (PPPI) networks of Arabidopsis thaliana orthologous proteins corresponding to resistant and susceptible interactions were obtained followed by cluster and gene ontology analyses. The integrated analysis of gene expression and systems biology allowed designing a general scheme of major mechanisms associated with witches' broom disease resistance/susceptibility. In this sense, the TSH1188 cultivar shows strong production of ROS and elicitors at the beginning of the interaction with M. perniciosa followed by resistance signal propagation and ROS detoxification. On the other hand, the Catongo genotype displays defense mechanisms that include the synthesis of some defense molecules but without success in regards to elimination of the fungus. This phase is followed by the activation of protein metabolism which is achieved with the production of proteasome associated with autophagy as a precursor mechanism of PCD. This work also identifies candidate genes for further functional studies and for genetic mapping and marker assisted selection. © 2012 The Royal Society of Chemistry.

Heim H.N.,Paranaense University | Vieira E.S.N.,Coodetec | Polo L.R.T.,Coodetec | Lima N.K.,Paranaense University | And 4 more authors.
Genetics and Molecular Research | Year: 2017

One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in genetic breeding programs besides the loss of valuable genetic material. Thus, this study aimed to evaluate the viability of a microsatellite marker as an internal amplification control with a dominant marker for the wheat Glu1-Dx5 gene. A population of 77 wheat cultivars/breeding lines was analyzed. Fourteen microsatellite markers were analyzed in silico regarding the formation of dimers and clamps. The biplex reaction conditions were optimized, and the Xbarc117 marker was selected as the internal amplification control with a Glu1-Dx5 marker in wheat. It was concluded that the Xbarc117 microsatellite marker was effective in the simultaneous amplification with a dominant Glu1-Dx5 marker, making biplex PCR viable in wheat for the studied markers. © 2017 The Authors.

Novaes T.G.,Instituto Agronomico do Parana | Almeida W.P.,Instituto Agronomico do Parana | Schuster I.,Coodetec | Aguiar P.,Fundacao MT | Mehta Y.R.,Instituto Agronomico do Parana
Summa Phytopathologica | Year: 2011

Areolate mildew, caused by Ramularia areola is an important disease affecting cotton in Brazil. A few years ago this disease was of secondary importance since it used to occur almost at the end of thecrop cycle. However, in recent years the disease has gained considerable importance in the main cotton growing areas of Brazil. Amongst control alternatives is the use of fungicides and resistant cultivars the latter being preferred. In order to study the inheritance of cotton resistance to R. areola, plant populations derived from the crossing between the resistant line FMT02102996 and the susceptible cultivar FMT 701, were evaluated. These plants were classified as resistant or susceptible by means of artificial inoculation in greenhouse. The segregation test was done through ÷2 test. Results indicated that cotton resistance to areo late mildew is controlled by a dominant gene. This finding can assist in planning cotton breeding programs aimed at incorporating resistance to R. areola in nem cultivars, constituting basic information for the beginning of the genetic mapping of this resistance gene in agro no mically desirable cotton cultivars.

de Franceschi L.,Federal Technological University of Paraná | Benin G.,Federal Technological University of Paraná | Marchioro V.S.,COODETEC | Martin T.N.,Federal Technological University of Paraná | And 2 more authors.
Bragantia | Year: 2010

This investigation had the objective to evaluate grain yield adaptability and stability of 17 wheat cultivars (Triticum aestivum L.) for Paraná State, Brazil, occording to four different methods. The experiments were carried out in 2007, at different locations of paraná, in complete randomized blocks experimental design, with 4 replicates. The Wricke (1965) methodology indicates stable cultivars, independently of average yield. EEBERHART and RRUSSELL (1966) and CRUZ et al. (1989) methodologies were equally efficient to evaluate stability and indicate cultivars that are stable and also adapted to favorable and unfavorable environments. LIN and BINNS (1988) methodology showed to be of easy interpretation and was efficient to indicate cultivars of high yield and with good stability, where more responsive materials showed a lesser Pi, and high negative correlation of Spearman between grain yields. It was concluded that LIN and BINNS methodology is very specific and when associated to Wi offers more assurance in recommending cultivars for high stability.

Marchioro V.S.,COODETEC | de Franco A.F.,COODETEC | Schuster I.,COODETEC | Dalla Nora T.,COODETEC | And 3 more authors.
Bioscience Journal | Year: 2015

Wheat breeding programs focus on potential grain yield, industrial quality, low plant height, and disease resistance. Cultivar CD 154 was a result of the search for cultivars with high yield potential and industrial wheat quality. This cultivar was derived from a cross between CD 104 and CDI 200104, by COODETEC, in 1999. Cultivar CD 154 was included in preliminary grain yield trials in 2005 and 2006, and was then tested to determine the Value of Cultivation and Use (VCU) from 2007 to 2012, with the acronym CD 0705. All trials were arranged in an experimental design of randomized blocks with three replications. The yield of cultivar CD 154 was 16 %, 10 % and 4 % higher than the mean yield of the two best controls, respectively, in the wheat-growing regions VCU 2, 3 and 4. The analysis of industrial quality, resulted in a mean gluten strength of 364 and mean stability of 17.1 min, which allows the inclusion cultivar CD 154 in the cultivar group of strong gluten wheat. The cultivar is classified as strong gluten wheat, has a high yield potential and good lodging resistance, and is therefore another promising alternative for wheat farmers of the hottest regions of Brazil. © 2015, Universidade Federal de Uberlandia. All rights reserved.

Franco F.A.,COODETEC | Marchioro V.S.,COODETEC | Dalla Nora T.,COODETEC | Schuster I.,COODETEC | And 2 more authors.
Bioscience Journal | Year: 2013

Grain yield potential and industrial quality are objectives of breeding programs, in this sense was developed the wheat cultivar CD 151. This cultivar was obtained from the cross between the cultivars BRS 120 and ORL 95282, by COODETEC in 1999. Cultivar CD 151, participated in the Preliminary Tests of grain yield in 2006 and 2007, and after that in trials for Determination of Value and Use (VCU) in the years 2008, 2009 and 2010, with the acronym CD 0818. All tests were conducted in randomized blocks, with three replications. The CD 151 cultivar showed a grain yield 12%, 7% and 9% higher than the average of the best two check cultivars, respectively, in wheat growing regions of VCU 2, 3 and 4. This cultivar has strong gluten wheat, high yield potential, and good resistance to leaf rust, powdery mildew, rice blast and lodging, resulting so in another important option for wheat growers in Brazil.

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