Contract Research Center

Mandsaur, India

Contract Research Center

Mandsaur, India
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Rathore C.,Contract Research Center | Dutt K.R.,Contract Research Center | Deb L.,Institute of Bioresources and Sustainable Development IBSD
Journal of Medicinal Plant Research | Year: 2011

In this study the methanolic leaves extract of Physalis angulata Linn (Solanaceae) (PAL) was investigated for anti-histaminic activity by usingisolated animal smooth muscle models (guinea pig ileum preparation, guinea pig trachea and rat fundus strip), where the plants extract possess inhibitory efficacy against histamine and 5 - HT. Acute toxicity study of the plant extract was also performed to measure the safety prospective. In smooth muscle models, PAL possesses inhibitory efficacy by 100% (1 μg), 133% (2 μg) and 126% (4 μg) in guinea pig ileum preparation (GPIP) against 1 μg histamine; 86% (1 μg), 100% (2 μg) and 106% (4 μg) in guinea pig tracheal chain preparation (GPTCP) against 1 μg histamine; and 50% (1 μg), 75% (2 μg) and 100% (4 μg) in fundus strip preparation (FSP) against 1 μg 5HT. A survey by the National Asthma Campaign found that 60% of the people with moderate asthma and 70% with severe asthma used complementary and alternative medicine to treat their conditions. Herbal medicine is the third most popular choice of both adults (11%) and children (6%) suffering from asthma, although P. angulata is used for the treatment of antihyper glycemic, anti-inflammatory, antimicrobial, antiseptic, antiviral, diuretic, expectorant and febrifuge. In traditional systems, there was only one claim for asthma. The present study will help the industry to produce herbal drug with less side effect, less cost and more effectiveness in the treatment of asthma. © 2011 Academic Journals.

Tiwari R.,Contract Research Center | Jain A.,Contract Research Center | Maliwal D.,Contract Research Center | Toppo E.,Contract Research Center
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

Multicriteria optimization methodology was applied for development of isocratic reversed-phased HPLC method for simultaneous determination of Indapamide and Perindopril. In the first stage of method development, pH value of the water phase, percentage of methanol, temperature of the column and flow rate of the mobile phase were investigated using fractional factorial design. This work is concerned with application of simple, accurate, precise and highly selective reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Indapamide and Perindopril in combined dosage form. Chromatographic separation was achieved isocratically at 25°C ± 0.5°C on phase Inertsil ODS C8 [250 mm x 4.6mm] 5°m column with a mobile phase composed of Phosphate buffer with pH 2.5 and methanol in the ratio of 40:60 at flow rate of 0.5 ml/min. Detection is carried out using a UV-PDA detector at 215nm. The retention time of Perindopril at and Indapamide at 5.08 min and 6.91 min respectively. The correlation coefficients for all components are close to 1. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of Indapamide and Perindopril in routine analysis.

Jain V.,Contract Research Center | Jain A.,Contract Research Center | Maliwal D.,Contract Research Center | Jain P.,Contract Research Center
Asian Journal of Chemistry | Year: 2010

A simple, rapid, sensitive and precise spectrophotometric methods in ultraviolet (UV) region and visible region has been developed for the estimation of cefprozil in bulk and pharmaceutical dosage forms. The visible method is based on formation of extractable coloured complex of drug with colouring agent. i.e., methyl red. Cefprozil showed maximum absorbance at 280 nm in UV region and at 536 nm in visible region. Beer's law was obeyed in concentration range of 5-40 μg/mL in UV region and 50-300 μg/mL in visible region. Regression equation was found to be y = 0.0276x + 0.0085 and coefficient of correlation was 0.9993 by UV method and y = 0.0049x - 0.0123 and coefficient of correlation was 0.9989. Results of the analysis were validated statistically and by recovery studies. The method is applied to marketed tablet formulation. Result of analysis of tablet formulation by both UV and visible, given as percentage of label claim ± standard deviation is 99.640 ± 1.198 and 99.920 ± 0.587, respectively. The precision and accuracy was examined by performing recovery studies and was found to be 99.150 ± 0.472 and 99.620 ±0.213 for both UV and visible, respectively. Sandell's sensitivity is calculated as 0.03640 and 0.0212 for both UV and visible spectrophotometry, respectively.

Jain S.,IPS Academy | Jain A.,Contract Research Center | Jain N.,IPS Academy | Jain D.K.,IPS Academy | Balekar N.,IPS Academy
Natural Product Research | Year: 2010

We evaluate the in vitro free radical scavenging activity of the leaves of Tabernaemontana divaricata Linn. Petroleum ether, ethanol and aqueous extracts of T. divaricata were prepared with successive extraction in a soxhlet apparatus. Each extract was selected to study the free radical scavenging activity by superoxide scavenging assay method. It was found that the aqueous extract contained carbohydrates, glycosides, amino acids, flavonoids, tannins, alkaloids, and steroids, and the ethanolic extract contained glycosides, amino acids, flavonoids, tannins, alkaloids and steroids. The ethanolic extract of T. divaricata showed 58.7 ± 0.62% inhibition in the superoxide scavenging model. The aqueous extract also showed almost similar activity (54.9 ± 0.53% compared to the ethanolic extract), while petroleum ether extract showed poor inhibition of superoxide scavenging activity. All extracts showed the dose- and time-dependent inhibition of the superoxide scavenging activity. © 2010 Taylor & Francis.

PubMed | Contract Research Center
Type: Journal Article | Journal: Pharmacognosy research | Year: 2011

The aim of the present study is to develop a polymer (Guar Gum)-based matrix tablet (using quercetin as a model drug) with sufficient mechanical strength, and promising in vitro mouth-to-colon release profile. By definition, an oral colonic delivery system should retard drug release in the stomach and small intestine, and allow complete release in the colon. By drug delivery to the colon would therefore ensure direct treatment at the disease site, lower dosing, and fewer systemic side effects. Quercetin is antioxidant in nature and used to treat colon cancer, but they have poor absorption in the upper part of the gastrointestinal tract (GIT). As a site for drug delivery, the colon offers a near neutral pH, reduced digestive enzymatic activity, a long transit time, and an increased responsiveness to absorption enhancers. By achieving a colon-targeted drug delivery system, the absorption of quercetin may be increased, which leads to better bioactivity in fewer doses.

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