Castiglioni E.,JASCO Corporation |
Castiglioni E.,University of Brescia |
Albertini P.,JASCO Europe |
Abbate S.,University of Brescia |
Abbate S.,Consorzio Nazionale Interuniversitario Science Fisiche della Materia
Chirality | Year: 2010
Because of the increased use of circular dichroism (CD) spectroscopy as a routine technique by nonspecialists to determine the conformational/ configurational properties of biomolecules, we have decided to present here some criteria to accurately check the ordinate scale calibration of a CD spectrometer particularly in the critical low-wavelength UV region, to understand, and correct, where possible, the potential limitations coming from the hardware. We also analyze some wavelength calibration methods, and some standards for the CD-scale calibration, and we discuss the critical characteristics of current instrumentation affecting measurements. The example of the bovine catalase CD spectrum is considered. Chirality, 2010. © 2010 Wiley-Liss, Inc. © Copyright © 2010 Wiley-Liss, Inc.