Consorzio Nazionale Interuniversitario Science Fisiche della Materia

Rome, Italy

Consorzio Nazionale Interuniversitario Science Fisiche della Materia

Rome, Italy
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Castiglioni E.,JASCO Corporation | Castiglioni E.,University of Brescia | Albertini P.,JASCO Europe | Abbate S.,University of Brescia | Abbate S.,Consorzio Nazionale Interuniversitario Science Fisiche della Materia
Chirality | Year: 2010

Because of the increased use of circular dichroism (CD) spectroscopy as a routine technique by nonspecialists to determine the conformational/ configurational properties of biomolecules, we have decided to present here some criteria to accurately check the ordinate scale calibration of a CD spectrometer particularly in the critical low-wavelength UV region, to understand, and correct, where possible, the potential limitations coming from the hardware. We also analyze some wavelength calibration methods, and some standards for the CD-scale calibration, and we discuss the critical characteristics of current instrumentation affecting measurements. The example of the bovine catalase CD spectrum is considered. Chirality, 2010. © 2010 Wiley-Liss, Inc. © Copyright © 2010 Wiley-Liss, Inc.

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