Sainte Genevieve, MO, United States
Sainte Genevieve, MO, United States

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Patent
Conagen Inc. | Date: 2016-12-01

Recombinant polypeptides having UDP-glycosyltransferase activities, including a 1,2-19-O-glucose glycosylation activity and a 1,2-13-O-glucose glycosylation activity for synthesizing of steviol glucosides, are provided. A method of producing a steviol glycoside composition using such recombinant polypeptide is also provided. Also disclosed are steviol glycosides referred to as rebaudioside Z1 and rebaudioside Z2.


A biosynthetic method of making a capsaicinoid including expressing a first gene product of CS/AT3/Pun1 in a cellular system, growing the cellular system in a medium, and collecting the capsaicinoid. The biosynthetic method further includes expressing a second gene product of ACS1 and expressing a third gene product of pAMT in the cellular system.


Grant
Agency: NSF | Branch: Standard Grant | Program: | Phase: | Award Amount: 225.00K | Year: 2016

The broader impact/commercial potential of this Small Business Technology Transfer (STTR) project will be the microbial production by fermentation of a group of natural food colorants, called anthocyanins, which will benefit the environment, the food industry, and society as a whole. The fermentation production process provides a green chemistry approach to issues usually encountered in traditional chemical synthesis, such as hazardous catalysts, and eliminates a solvent waste problem typically associated with extraction of natural compounds from botanicals. The proposed work will lead to a greater understanding of the anthocyanin biosynthetic pathway, and result in a lower cost of production of these compounds. Innovation and understanding of the biochemical pathways and process optimization will help serve the potentially multi-billion dollar global market for high purity natural food colorants as food ingredients, as health care products, and in therapeutic applications.

This STTR Phase I project proposes to engineer microorganisms that are Generally Regarded As Safe (GRAS), and are currently used for the production of several food additives as microbial cell factories for the production of various anthocyanin molecules. Preliminary work on the biosynthesis of the anthocyanins, using metabolically engineered Escherichia coli recombinant strains, has demonstrated feasibility of this bio-economic approach. Initial work will involve the introduction of plant metabolic pathways in microorganisms via various gene-splicing methods. Further process optimization will be undertaken by improving fermentation conditions and by performing an initial scale-up using the recombinant microorganisms. This microbial fermentation strategy will help enhance the production of such beneficial natural food colorants through a biomanufactuing approach utilizing principles of synthetic biology and metabolic engineering and other emerging biotechnologies.


Grant
Agency: National Science Foundation | Branch: | Program: STTR | Phase: Phase I | Award Amount: 225.00K | Year: 2016

The broader impact/commercial potential of this Small Business Technology Transfer (STTR) project will be the microbial production by fermentation of a group of natural food colorants, called anthocyanins, which will benefit the environment, the food industry, and society as a whole. The fermentation production process provides a "green chemistry" approach to issues usually encountered in traditional chemical synthesis, such as hazardous catalysts, and eliminates a solvent waste problem typically associated with extraction of natural compounds from botanicals. The proposed work will lead to a greater understanding of the anthocyanin biosynthetic pathway, and result in a lower cost of production of these compounds. Innovation and understanding of the biochemical pathways and process optimization will help serve the potentially multi-billion dollar global market for high purity natural food colorants as food ingredients, as health care products, and in therapeutic applications. This STTR Phase I project proposes to engineer microorganisms that are Generally Regarded As Safe (GRAS), and are currently used for the production of several food additives as microbial cell factories for the production of various anthocyanin molecules. Preliminary work on the biosynthesis of the anthocyanins, using metabolically engineered Escherichia coli recombinant strains, has demonstrated feasibility of this bio-economic approach. Initial work will involve the introduction of plant metabolic pathways in microorganisms via various gene-splicing methods. Further process optimization will be undertaken by improving fermentation conditions and by performing an initial scale-up using the recombinant microorganisms. This microbial fermentation strategy will help enhance the production of such beneficial natural food colorants through a biomanufactuing approach utilizing principles of synthetic biology and metabolic engineering and other emerging biotechnologies.


Patent
Conagen Inc. | Date: 2015-10-02

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).


Patent
Conagen Inc. | Date: 2016-06-03

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).


Patent
Conagen Inc. | Date: 2016-01-19

Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).


Patent
Conagen Inc. | Date: 2016-02-05

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five -D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.


Patent
Conagen Inc. | Date: 2016-02-05

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five -D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.


Patent
Conagen Inc. | Date: 2016-02-05

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five -D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.

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