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Lago N.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | Legido J.L.,University of Vigo | Paz Andrade M.I.,University of Santiago de Compostela | Arias I.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | Casas L.M.,University of Vigo
Journal of Thermal Analysis and Calorimetry | Year: 2011

Microcalorimetry is an experimental technique which allows us to precisely measure the energy released as a consequence of any transformation process. All organisms produce heat as a consequence of metabolism. The rate of heat production is an adequate measurement of metabolic activity of organisms and their constituent parts, cells and sub-cellular levels. Microorganisms produce small amounts of heat, in the order of 1-3 pW per cell. Despite the low quantity of heat produced by bacteria, their exponential replication in culture medium allows their detection using microcalorimetry. This study is a microcalorimetric study of the growth and metabolism of the bacterium Pseudomonas aeruginosa, using the heat liberated as a consequence of bacterial metabolism. With this aim, we used a Calvet microcalorimeter, inside which two Teflon screw-capped stainless steel cells were located (sample and reference). Experiments were carried out at final concentrations of 106, 105, 10 3 and 10 CFU/mL, and a constant temperature of 309.65 K was maintained within the microcalorimeter. Recording the difference in calorific potential over time we obtained P. aeruginosa's growth curves. The shape of these curves is characteristic and has a single phase. Thus, the heat flow curves were mathematically studied to calculate the growth constant and generation time of this bacterium. © 2010 Akadémiai Kiadó, Budapest, Hungary.


Rivero N.L.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | Soto J.L.L.,University of Vigo | Casas L.M.,University of Vigo | Santos I.A.,Complejo Hospitalario Universitario Of Vigo Xeral Cies
Journal of Thermal Analysis and Calorimetry | Year: 2012

Enterococcus faecalis is a Gram-positive bacteria, considered one of the most common causes of nosocomial infections. Bacterial cultures produce an exchange of energy as a result of the bacteria metabolisms. The rate of heat production is an adequate measure of the metabolic activity of the organisms and their constituent parts. Microorganisms produce small amounts of heat: 1-3 pW per cell. Although the heat produced by bacteria is very small, their exponential reproduction in a culture medium permits heat detection through microcalorimetry. In this study, we analyzed the microcalorimetric behavior of Enterococcus faecalis. A thermal Calvet microcalorimeter was used. The inside of the calorimeter contains two stainless steel cells (experimental and reference). Experiments were carried out at final concentrations of 10 6,10 5,10 3, and 10 CFU/mL and a constant temperature of 309.65 K was maintained within the microcalorimeter. Recording the difference in calorific potential over time we obtained E. faecalis's growth curves. Thermograms were analyzed mathematically allowing us to calculate the constant growth, generation time and the amount of heat exchanged over the culture time. © Akadémiai Kiadó, Budapest, Hungary 2011.


Lago Rivero N.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | Mucientes Molina A.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | Paradela Carreiro A.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | Vazquez Gomez C.,Complejo Hospitalario Universitario Of Vigo Xeral Cies | And 3 more authors.
Nutricion Hospitalaria | Year: 2012

Patient diagnosed with Crohn's Disease with inflammatory pattern that evolves stenosing-piercing, causing abdominal perforation and fecal peritonitis. She was underwent to three surgeries, leading to numerous complications and a torpid clinical course. Given the state of malnutrition on admission it was prescribed Total Parenteral Nutrition (TPN), extending the administration for more than 10 months. In this period the TPN is suspended for 5 days, but the persistence of an enterocutaneous fistula causes the restoration of the TPN. After clinical stabilization, the patient is discharged to recover her nutritional status necessary to perform a bowel reconstruction surgery, continuing with TPN at home. After 7 and a half months, the patient with an optimal nutritional status, undergoes surgery, evolving favorably and suspending the TPN at 9 days.

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