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Chang Y.-C.,Muroran Institute of Technology | Takada K.,Muroran Institute of Technology | Choi D.,Company Randnter | Choi D.,Chungbuk National University | And 3 more authors.
Applied Biochemistry and Biotechnology | Year: 2013

Four strains of biphenyl-degrading bacteria were isolated from a sewage and identified from the Rhodococcus genus (SK-1, SK-3, and SK-4) and Aquamicrobium genus (SK-2) by 16S rRNA sequence. Among these strains, strain SK-2 was most suitable for biphenyl degradation. When 0.65, 1.3, 2.6, or 3.9 mM of biphenyl was used, the biphenyl was completely degraded within 24 and 96 h of culture, respectively. However, in the case of 6.5 and 9.75 mM of biphenyl, the biphenyl degradation yields were about 80 % and 46.7 % after 120 h of culture, respectively. The isolated strains could degrade a broad spectrum of aromatic compounds including high-chlorinated polychlorinated biphenyl (PCB) congeners in the presence of biphenyl. In addition, strain SK-2 could utilize PCB congeners containing one to six chlorine substituents such as 2,2′,4,4′,5, 5′-hexachlorobiphenyl. The PCB utilization rate by the strain SK-2 was increased compared to that of other PCB congener-utilizing bacteria. The four isolates metabolized 4-chlorobiphenyl to 4-chlorobenzoic acid and 2-hydroxy-6-oxo-6-(4′-chlorophenyl)-hexa-2,4-dienoic acid. These results suggest the isolated strains might be good candidates for the bioremediation of PCB-contaminated soil, especially high-saline soils. © 2013 Springer Science+Business Media New York.


Kim B.H.,Chosun University | Choi D.,Chungbuk National University | Choi D.,Company R and nter | Piao Y.L.,Chosun University | And 5 more authors.
Korean Journal of Chemical Engineering | Year: 2012

We investigated the effects of the antioxidant and the nitrite scavenging activities of the extracts from Pleurotus ferulae fruiting body grown on the solid state using corn cob and activated bleaching earth (CCABE media) and its mycelium grown in the liquid state. The total phenol and polysaccharide concentrations in hot water extract of fruiting body were approximately 3.6- and 4.3-fold higher than those of the mycelium. Using the hot water extract of fruiting body, the maximum DPPH radical scavenging activity at 9 mg/mL, hydroxyl radical scavenging activity at 12mg/mL, reducing power at 12 mg/mL, and chelating ability at 12 mg/mL were obtained, 80.5%, 72.4%, 0.99 OD (700 nm), and 77.0%, respectively. However, in the case of hydrogen peroxide scavenging activity, the ethanol extract was the highest, 78.7% at 12 mg/mL. The maximum nitrite scavenging activity was obtained, 89.7% at 6 mg/mL of hot water extract from fruiting body. Hot water extracts were more effective than ethanol extracts in scavenging activity on DPPH radicals and hydroxyl radical scavenging, reducing power, and chelating activity of ferrous, whereas ethanol extracts were more effective in hydrogen peroxide scavenging activity as evidenced by their lower EC 50 values. These results indicate that the hot water extract of P. ferulae fruiting body using CCABE media has good potential to be used as a source of materials or additives for oxidation suppressant in food, cosmetics and drug compositions. © 2012 Korean Institute of Chemical Engineers, Seoul, Korea.


Chang Y.-C.,Muroran Institute of Technology | Choi D.,Company Randnter | Choi D.,Chungbuk National University | Kikuchi S.,Muroran Institute of Technology
Bioresource Technology | Year: 2012

For biological extraction of heavy metals from chromated copper arsenate (CCA) treated wood, different bacteria were investigated. The extraction rates of heavy metals using Lactobacillus bulgaricus and Streptococcus thermophilus were highest. The chemical extraction rates were depended on the amounts of pyruvic acid and lactic acid. Especially, the extraction rates using mixed pyruvic acid and lactic acid were increased compared to those of sole one. They were also enhanced in the mixed culture of L. bulgaricus and S. thermophilus. To improve the extraction of CCA, a two-step processing procedure with the mixed culture of L. bulgaricus and S. thermophilus was conducted. A maximum of 93% of copper, 86.5% of chromium, and 97.8% of arsenic were extracted after 4. days. These results suggest that a two-step process with the mixed culture of L. bulgaricus and S. thermophilus is most effective to extract heavy metals from CCA treated wood. © 2011 Elsevier Ltd.


Choi D.,Chungbuk National University | Choi D.,Company R and nter | Choi O.-Y.,Chodang University | Park J.,Chosun University | And 2 more authors.
Korean Journal of Chemical Engineering | Year: 2011

For the functional cosmetic agent using acetone extract of A. sinicus Linne seed, the effects of whitening, wrinkling, and safety were investigated. Cell viabilities of Raw 264.7 up to 60 mg/mL did not appear to have any significant direct cytotoxic effect. The melanin concentration was decreased up to 62.1% at 20 mg/mL. When the acetone extract concentration of A. sinicus Linne seed was increased from 5 to 20 mg/mL, the inhibitory activity of tyrosinase was sharply increased from 61.3 to 93.8%. However, above 30 mg/mL, it did not increase. The inhibition effects of elastase and collagenase were increased with the extract concentration. Especially, when acetone extract concentration of A. sinicus Linne seed was increased from 25 to 200 μg/mL, the inhibition effect of elastase was increased from 60.2 to 97.5%. The inhibition effect of collagenase was increased from 35.0 to 99.0% when increased from 50 to 300 μg/mL. The indexes of pigment and coarseness were 28.56 MI and 18. 45R-value, respectively, after 8 weeks of clinical trial using cream pack containing 0.2% of acetone extract of A. sinicus Linne seed. The indexes of elasticity and moisture were 64.5Ur/Uf and 55.2AU, respectively, after 8 weeks of clinical trial. These results demonstrate that acetone extract of A. sinicus Linne seed may be useful as a potential agent for functional cosmetics. © 2010 Korean Institute of Chemical Engineers, Seoul, Korea.


Choi D.,Company R and nter | Choi D.,Chungbuk National University | Kim Y.-S.,Chosun University | Nam H.G.,Chosun University | And 5 more authors.
Korean Journal of Chemical Engineering | Year: 2011

The purpose of this study was to evaluate the effect of hot water extracts of a fish, seaweed, and mushroom mixture (FSM extract) on the antioxidant and anticancer activities for use as health and functional food resources. DPPH radical scavenging activity increased from 13.4 to 93.5% when the FSM extract concentration ranged from 0.5 to 25mg/mL. The reducing power increased from 0.04 to 1.06 OD 700 nm when the FSM extract concentration increased from 0.25 to 10mg/mL. Nitrite scavenging activity increased from 10.3 to 96.9% when the FSM extract concentration increased from 1 to 25mg/mL. The activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the FSM extractfed group were 2.45 and 6.12 units/min, respectively. The activities of CAT, SOD, and GSH-Px in the FSM extractfed group were 19.7 units/mg protein, 11.5 units/mg protein, and 16.9 units/mg protein/min, respectively. Cell viabilities of SNU213, SNU324, and SNU354 were 7.5, 9.4, and 8.9%, respectively. Cell viabilities of SNU719, SNU1, and SNU5 ranged from 14.6 to 16.8%. However, for SNU216, SNU484, SNU601, SNU638, SNU668, SNU16, and SNU520, they were below10%. These results demonstrate that the FSM extract can be used in the functional food, pharmaceutical, and cosmetic industries. © 2011 Korean Institute of Chemical Engineers, Seoul, Korea.


Lim D.-H.,Chosun University | Choi D.,Company R and nter | Choi D.,Chungbuk National University | Choi O.-Y.,Chodang University | And 4 more authors.
Journal of Industrial and Engineering Chemistry | Year: 2011

Antioxidant activity of various extracts of Astragalus sinicus L. seed was evaluated in vitro and in vivo. Among the various extracts, the acetone extract showed the maximum DPPH radical scavenging activity of 95.1% at 10.0. mg/mL, hydroxyl radical scavenging activity of 89.4% at 15. mg/mL, and superoxide anion scavenging activity of 74.5% at 15. mg/mL. The inhibition of xanthine oxidase and chelating effect at 10.0. mg/mL of acetone extract were found to be 95.1 and 93.2%, respectively. The concentrations of total phenolic and flavonoide were, respectively, 279.9 and 175.2. mg/g in the acetone extract, about 4.1 and 3.4 times higher than those of the water extract. When the administered concentration of acetone extract was 5.0. g/kg/body weight/day in rats, catalase, superoxide dismutase, and glutathione peroxidase activities of the liver were increased approximately 50% compared to those of the control groups. These results suggest that the acetone extract of A. sinicus L. seed might be a valuable antioxidant of natural source applicable in the medicine, cosmetics and the food industry. © 2011 The Korean Society of Industrial and Engineering Chemistry.


Gadhe C.G.,Chosun University | Lee S.H.,Chosun University | Madhavan T.,Chosun University | Kothandan G.,Chosun University | And 2 more authors.
Bulletin of the Korean Chemical Society | Year: 2010

In this study, we have developed QSAR models for a series of 38 piperidine-4-carboxamide CCR5 antagonists using CoMFA, CoMSIA and HQSAR methods. Developed models showed good statistics in terms of q2 and r2 values. Best predictions obtained with standard CoMFA model (r2 = 0.888, q2 = 0.651) and combined CoMSIA model (r2 = 0.892, q2 = 0.665) with electrostatics and H-bond acceptor parameter. The validity of developed models was assessed by test set of 9 compounds, which showed good predictive correlation coefficient for CoMFA (0.804) and CoMSIA (0.844). Bootstrapped analysis showed statistically significant and robust CoMFA (0.968) and CoMSIA (0.936) models. Best HQSAR model was obtained with a q2 of 0.662 and r2 of 0.936 using atom, connection, hydrogen, donor and acceptor as parameters and fragment size (7-10) with optimum number of 6 components. Predictive power of developed HQSAR model was proved by test set and it was found to be 0.728.

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