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Yu R.,Zhejiang University | Y-Hua L.,Community Health Service Center | Hong L.,Zhejiang University
International Journal of Diabetes in Developing Countries | Year: 2010

Objective: To investigate the incidence of depression in newly diagnosed type 2 diabetes. Materials and Methods: One hundred newly diagnosed (4 - 12 weeks) T2DM participants were evaluated for depressive symptoms by using the Self-Rating Depression Scale (SDS). Blood glucose (HBA1C), urinary albumin, BMI, and blood pressure were measured. Sexual function was evaluated by a self-score on IIEF-5 Questionnaires in male participants below 60 years of age. Results: Twenty-eight (28%) of these had depressive scores, 18 (18%) had mild scores, six (6%) had moderate scores, and four had (4%) severe ones. In those who took oral medication, the percentage of depression was 18.5% (10/54) and in those who were treated by insulin the percentage was 39.1% (18/46). The levels of fasting blood glucose (FBG), HBA1c, and urinary albumin were higher in those with depression. The SDS score was negatively correlated with age and annual household income (r = 0.151,0.139, P < 0.05); 17% of the (8/48) males below 60 years of age was diagnosed with erectile dysfunction (ED) by II EF-5score < 20 and the severity of depressive symptoms was negatively correlated with II EF-5score (r = 0.131, P < 0.05). Conclusion: These findings indicated that depressive symptoms are common in newly diagnosed type 2 diabetics. A variety of factors could be influencing the severity of depressive symptoms. Source


Zou C.-L.,Community Health Service Center
Journal of Acupuncture and Tuina Science | Year: 2014

Objective: To observe the effect of warm needling therapy plus acupuncture on third lumbar vertebra transverse process syndrome. Methods: Sixty cases of third lumbar vertebra transverse process syndrome were randomly divided into a warm needling group or an acupuncture group, 30 cases in each group. Cases in the acupuncture group were treated by routine points and needle sticking manipulation, while those in the warm needling group were treated by moxibustion plus needling as same as the acupuncture group. Japanese orthopedic association scores (JOA) in the two groups were observed and compared after 2 treatment courses. Results: After treatment, the JOA score in the warm needling group was higher than that in the acupuncture group (P<0.01); subjective symptom, clinical syndromes, activity of daily living (ADL) in the warm needling group were all higher than those in the acupuncture group (all P<0.05); the score of pain in the warm needling group was significantly lower than that in the acupuncture group (P<0.01); the total effective rate in the warm needling group was 76.7%, versus 70.0% in the acupuncture group, showing a statistically significant difference (P<0.05). Conclusion: Warm needling therapy is more effective than normal acupuncture in treating third lumbar vertebra transverse process syndrome. © 2014 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg. Source


Zhang X.,Wenzhou University | Ge X.,Wenzhou University | Yu Y.,Community Health Service Center | Zhang Y.,Wenzhou University | And 6 more authors.
Scientific Reports | Year: 2014

Idiopathic congenital nystagmus (ICN) consists of involuntary and periodic ocular motility, often with seriously reduced visual acuity. To identify the genetic defects associated with X-linked ICN, we performed PCR-based DNA direct sequencing of two candidate genes, FRMD7 and GPR143, in four families. Mutation analysis led to identification of three novel mutations, p.S260R, p.Q487X, and p.V549Y fsX554, in FRMD7 in three of the recruited families. Results from structural modeling indicated that the p.S260R may potentially disrupt FRMD7 function through loss of a phosphorylation site and/or interference with protein-protein interactions. Both p.Q487X, and p.V549Y fsX554 mutations were predicted to generate nonfunctional truncated proteins. Using a capture next generation sequencing method, we excluded CASK as the responsible gene for the remaining family. Combining sequence analysis and structural modeling, we report three novel mutations in FRMD7 in three independent families with XLICN, and provide molecular insights for future XLICN diagnosis and treatment. Source


Tong P.,Zhejiang Chinese Medical University | Tong P.,National Clinical Research Base of Traditional Chinese Medicine | Wu C.,Zhejiang Chinese Medical University | Wang X.,Wenzhou Hospital of Traditional Chinese Medicine | And 6 more authors.
Journal of Ethnopharmacology | Year: 2013

Ethnopharmacological relevance: Fuzi (lateral root of Aconitum carmichaeli) is a popular traditional Chinese medicine well known for its both therapeutic and high-toxic activities. Its toxic alkaloid ingredients, mainly aconitine, mesaconitine, and hypaconitine, are responsible for the high toxicity. However, to date, no detoxication strategy is available to completely eliminate Fuzi's toxicity, and, whether Fuzi's efficacy could be kept after detoxication, remain unknown and debatable. Materials and methods: The purpose of this study was to establish and validate a complete-detoxication strategy for Fuzi via acute toxicity test, to clarify the detoxication mechanism by HPLC and titrimetric analyses, and to evaluate the therapeutic effect of detoxicated Fuzi on adjuvant arthritis (AA). Three processed Fuzi (Bai-fu-pian) with 30-min, 60-min, and 120-min decoctions, respectively, named dBfp-30, dBfp-60, and dBfp-120, were prepared for this study. For the acute toxicity test, their oral doses to male and female Kunming mice were up to 70-190 g/kg body weight, and their toxicological profiles were evaluated by median lethal dose (LD50), maximal tolerance dose (MTD), minimal lethal dose (MLD), no-observed-adverse- effect-level (NOAEL), and time-concentration-mortality (TCM) modeling methods using a 14-day schedule with up to five doses. The HPLC analysis was performed to determine the detoxication-induced changes in composition and amount of aconitine, mesaconitine and hypaconitine in Fuzi, whilst the titrimetric method was adopted to estimate the amount changes of Fuzi's total alkaloids. AA model was established by incomplete Freund's adjuvant injection in Wistar rats, and the animal's physiological (body weight, food intake, etc.), clinical (hind paw volume), and immunological (IL-1 and TNF-α) parameters were assessed as markers of inflammation and arthritis. Results: With increasing decoction time, the acute toxicity of detoxicated Fuzi became decreased in the following order: dBfp-30 (LD50 of 145.1 g/kg; MTD of 70 g/kg; MLD of 100 g/kg; NOAEL of 70 g/kg) >dBfp-60 (too large LD50; MTD of 160 g/kg; MLD of 190 g/kg; NOAEL of 100 g/kg) >dBfp-120 (no LD50; unlimited MTD; unlimited MLD; NOAEL of 130 g/kg). dBfp-30 and dBfp-60 displayed the toxicity at a dose-dependent manner with maximum mortalities reaching 100% and 50% respectively, whereas no mortality or signs of intoxication was induced by dBfp-120. The chemical analyses revealed a dramatic reduction of the toxic alkaloids as well as total alkaloids in Fuzi after the detoxication, from which no level of aconitine and only minimum residual of mesaconitine (0.56±0.02 μg/g) and hypaconitine (8.73±0.13 μg/g) were detected in dBfp-120. However, no significant difference of total alkaloid amount was found among dBfp-30, dBfp-60, and dBfp-120 (P>0.05), suggesting an equivalent conversion from toxic alkaloids to its non-toxic derivants in dBfp-120. Further, also no significant differences were seen among dBfp-30, dBfp-60, and dBfp-120 for the therapeutic effects on physiological, clinical, and immunological parameters in AA rat, indicating that dBfp-120 is of non-toxicity and efficacy. Conclusions: A complete-detoxication strategy has been developed successfully for ensuring the safe and effective use of Fuzi. The detoxication mechanism associated with elimination of toxic alkaloids has kept Fuzi's efficacy, indicating a non-interdependent relationship between its efficacy and toxicity. This is the first report on such an optimal detoxication strategy and on the application of detoxicated Fuzi in AA. It may provide in depth understanding to the toxicological and pharmacological profiles of Fuzi and further benefit the herbal drug development with safety and efficacy for disease especially RA therapy. © 2013 Elsevier Ireland Ltd. Source


Yang Y.-F.,Community Health Service Center | Pu B.-H.,Community Health Service Center
World Chinese Journal of Digestology | Year: 2014

Gastroesophageal reflux disease is a clinically common refractory chronic disease of the digestive tract, with elusive pathogenesis and poor clinical curative effect. Scholars have developed various animal models to study this disease, and the animal models are prepared mainly by changing gastrointestinal tract anatomy to induce reflux esophagitis or by exogenously pouring acid to induce esophagitis. The former includes damage to the lower esophageal sphincter, pyloric ligation of the duodenum, anastomosis of the esophagus with the duodenum or lower esophagus jejunum, and mechanical expansion. Various preparation methods can induce different degrees of esophageal lesions, but each has its disadvantages in the actual research. The development of animal models should be based on the experimental purposes and requirements to choose suitable animals and reasonable operation, and take effective measures so as to prevent postoperative complications and lower mortality, improve the utilization rate, and prepare ideal animal models to meet the needs of research. © 2014 Baishideng Publishing Group Inc. All rights reserved. Source

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