PubMed | Karolinska Institutet, Community Health Service Center and Tianjin Medical University
Type: Journal Article | Journal: The journals of gerontology. Series A, Biological sciences and medical sciences | Year: 2016
This study is to examine the effects of folic acid supplementation on cognitive function in Chinese older adults with mild cognitive impairment who are unexposed to folic acid fortification and assess cognitive functioning in relation to folate, homocysteine, and vitamin B12 values at baseline.This was a single-center, randomized, controlled trial in Tianjin, China; 180 individuals aged 65 years and older who had mild cognitive impairment were assigned randomly to one of two groups: (a) those treated with oral folic acid (400 g/day) and (b) those treated via conventional treatment. Tests of cognitive performance and biomarkers were measured at baseline, 3 months, and 6 months. Analysis was by intention-to-treat. Changes in cognitive or clinical function were analyzed by repeated-measure analysis of variance or mixed-effects models. This trial has been registered with the trial number ChiCTR-TRC-13003227.Total of 159 participants (intervention group: 80; control group: 79) completed the trial. Repeated-measure analysis of variance showed significant improvements in serum folate (p (2) = 0.712, p = .009), homocysteine (p (2) = 0.119, p = .017), serum vitamin B12 (p (2) = 0.144, p = .022), and S-adenosylmethionine (p (2) = 0.117, p = .033) in the intervention group over the control group. Folic acid supplementation improved Full Scale IQ (p = .031; effect size d = 0.168), Digit Span (p = .009; d = 0.176), and Block Design (p = .036; effect size d = 0.146) scores at 6 months in comparison to the control. There were no significant findings for all other cognitive measures.There was a beneficial effect from relatively short-term folate supplementation on cognitive functioning in later life. Larger-scale, randomized, controlled trials of longer duration in selected age groups are needed.
PubMed | Guangzhou General Hospital Of Pla, Community Health Service Center, Shanghai Medical Science & Technology Information Center, Fudan University and 3 more.
Type: Journal Article | Journal: BMC geriatrics | Year: 2016
Both multimorbidity and activities of daily living (ADL) disability and instrument activities of daily living (IADL) disability are common among elderly individuals. ADL/IADL disability may reduce individuals capacities for independent living and quality of life. This study aimed to examine the association between multimorbidity and ADL/IADL disability.A multi-stage cluster sample of 2058 residents aged 80 or older was investigated in Shanghai, China. Multimorbidity was defined as the simultaneous presence of two or more chronic diseases with ten common chronic conditions under consideration. Subjects who responded that they need partial or full assistance to any ADL/IADL items were defined as having ADL/IADL disability. We examined the association of multimorbidity with ADL/IADL disability, adjusted for socio-demographic characteristics by using logistic regression.Of respondents, 23.23% had ADL disability, 37.90% had IADL disability, and 49.17% had multimorbidity. After adjusted socio-demographic characteristics, a graded association was showed between ADL disability and the quantity of chronic conditions: odds ratio (OR) for 1 condition, 1.53(95% confidence interval [CI], 1.04-2.24); OR for 2 conditions, 2.06(95% CI, 1.43-2.96); OR for 3 conditions, 3.23(95% CI, 2.14-4.86); OR for 4 or more conditions, 5.61(95% CI, 3.26-9.66). Similar associations were also observed between the quantity of chronic conditions and IADL disability.The quantity of chronic conditions had relatively strong association with both ADL and IADL disability. Initiating prevention of additional chronic conditions and interventions on clusters of diseases may decrease the potential risk of ADL/IADL disability. Additionally, more attention should been given to the older low-income women living with relatives/non-relatives with multimorbidity.
PubMed | Centers for Disease Control and Prevention, Community Health Service Center, Shanghai Institute of Technology, Fudan University and 3 more.
Type: | Journal: International psychogeriatrics | Year: 2016
Previous studies have thoroughly investigated the prevalence and risk factors for completed suicide. In marked contrast is the lack of a better understanding of attempted suicide in the elderly. The aim of this study was to estimate the prevalence of attempted suicide in the elderly and examine the associated factors.Using a multi-stage cluster sampling approach, a cross-sectional survey of 8,399 elderly house-dwelling residents was conducted in Shanghai, China.The two-week prevalence of attempted suicide in the elderly was 0.75%. In the bivariate analysis, having no caregivers, depressive, anxiety, sad, fear, obsessive-compulsive and anger symptom, and lower scores on the Barthel Index of Activities of Daily Living and the Lawton Instrumental Activities of Daily Living Scale were significantly associated with an increased risk of attempted suicide in the elderly. In the multivariate analysis, sad and fear symptoms were significantly and independently associated with a higher risk of attempted suicide in the elderly.The two-week prevalence of attempted suicide in the elderly is relatively high when compared with the annualized or lifetime prevalence reported in China and foreign settings. Elderly individuals with certain mental symptoms should be targeted for suicide prevention and provided with timely mental health support.
Tong P.,Zhejiang Chinese Medical University |
Tong P.,Zhejiang Provincial Hospital of Traditional Chinese Medicine |
Wu C.,Zhejiang Chinese Medical University |
Wang X.,Wenzhou Hospital of Traditional Chinese Medicine |
And 6 more authors.
Journal of Ethnopharmacology | Year: 2013
Ethnopharmacological relevance: Fuzi (lateral root of Aconitum carmichaeli) is a popular traditional Chinese medicine well known for its both therapeutic and high-toxic activities. Its toxic alkaloid ingredients, mainly aconitine, mesaconitine, and hypaconitine, are responsible for the high toxicity. However, to date, no detoxication strategy is available to completely eliminate Fuzi's toxicity, and, whether Fuzi's efficacy could be kept after detoxication, remain unknown and debatable. Materials and methods: The purpose of this study was to establish and validate a complete-detoxication strategy for Fuzi via acute toxicity test, to clarify the detoxication mechanism by HPLC and titrimetric analyses, and to evaluate the therapeutic effect of detoxicated Fuzi on adjuvant arthritis (AA). Three processed Fuzi (Bai-fu-pian) with 30-min, 60-min, and 120-min decoctions, respectively, named dBfp-30, dBfp-60, and dBfp-120, were prepared for this study. For the acute toxicity test, their oral doses to male and female Kunming mice were up to 70-190 g/kg body weight, and their toxicological profiles were evaluated by median lethal dose (LD50), maximal tolerance dose (MTD), minimal lethal dose (MLD), no-observed-adverse- effect-level (NOAEL), and time-concentration-mortality (TCM) modeling methods using a 14-day schedule with up to five doses. The HPLC analysis was performed to determine the detoxication-induced changes in composition and amount of aconitine, mesaconitine and hypaconitine in Fuzi, whilst the titrimetric method was adopted to estimate the amount changes of Fuzi's total alkaloids. AA model was established by incomplete Freund's adjuvant injection in Wistar rats, and the animal's physiological (body weight, food intake, etc.), clinical (hind paw volume), and immunological (IL-1 and TNF-α) parameters were assessed as markers of inflammation and arthritis. Results: With increasing decoction time, the acute toxicity of detoxicated Fuzi became decreased in the following order: dBfp-30 (LD50 of 145.1 g/kg; MTD of 70 g/kg; MLD of 100 g/kg; NOAEL of 70 g/kg) >dBfp-60 (too large LD50; MTD of 160 g/kg; MLD of 190 g/kg; NOAEL of 100 g/kg) >dBfp-120 (no LD50; unlimited MTD; unlimited MLD; NOAEL of 130 g/kg). dBfp-30 and dBfp-60 displayed the toxicity at a dose-dependent manner with maximum mortalities reaching 100% and 50% respectively, whereas no mortality or signs of intoxication was induced by dBfp-120. The chemical analyses revealed a dramatic reduction of the toxic alkaloids as well as total alkaloids in Fuzi after the detoxication, from which no level of aconitine and only minimum residual of mesaconitine (0.56±0.02 μg/g) and hypaconitine (8.73±0.13 μg/g) were detected in dBfp-120. However, no significant difference of total alkaloid amount was found among dBfp-30, dBfp-60, and dBfp-120 (P>0.05), suggesting an equivalent conversion from toxic alkaloids to its non-toxic derivants in dBfp-120. Further, also no significant differences were seen among dBfp-30, dBfp-60, and dBfp-120 for the therapeutic effects on physiological, clinical, and immunological parameters in AA rat, indicating that dBfp-120 is of non-toxicity and efficacy. Conclusions: A complete-detoxication strategy has been developed successfully for ensuring the safe and effective use of Fuzi. The detoxication mechanism associated with elimination of toxic alkaloids has kept Fuzi's efficacy, indicating a non-interdependent relationship between its efficacy and toxicity. This is the first report on such an optimal detoxication strategy and on the application of detoxicated Fuzi in AA. It may provide in depth understanding to the toxicological and pharmacological profiles of Fuzi and further benefit the herbal drug development with safety and efficacy for disease especially RA therapy. © 2013 Elsevier Ireland Ltd.
Zhang X.,Wenzhou University |
Ge X.,Wenzhou University |
Yu Y.,Community Health Service Center |
Zhang Y.,Wenzhou University |
And 6 more authors.
Scientific Reports | Year: 2014
Idiopathic congenital nystagmus (ICN) consists of involuntary and periodic ocular motility, often with seriously reduced visual acuity. To identify the genetic defects associated with X-linked ICN, we performed PCR-based DNA direct sequencing of two candidate genes, FRMD7 and GPR143, in four families. Mutation analysis led to identification of three novel mutations, p.S260R, p.Q487X, and p.V549Y fsX554, in FRMD7 in three of the recruited families. Results from structural modeling indicated that the p.S260R may potentially disrupt FRMD7 function through loss of a phosphorylation site and/or interference with protein-protein interactions. Both p.Q487X, and p.V549Y fsX554 mutations were predicted to generate nonfunctional truncated proteins. Using a capture next generation sequencing method, we excluded CASK as the responsible gene for the remaining family. Combining sequence analysis and structural modeling, we report three novel mutations in FRMD7 in three independent families with XLICN, and provide molecular insights for future XLICN diagnosis and treatment.
Yang Y.-F.,Community Health Service Center |
Pu B.-H.,Community Health Service Center
World Chinese Journal of Digestology | Year: 2014
Gastroesophageal reflux disease is a clinically common refractory chronic disease of the digestive tract, with elusive pathogenesis and poor clinical curative effect. Scholars have developed various animal models to study this disease, and the animal models are prepared mainly by changing gastrointestinal tract anatomy to induce reflux esophagitis or by exogenously pouring acid to induce esophagitis. The former includes damage to the lower esophageal sphincter, pyloric ligation of the duodenum, anastomosis of the esophagus with the duodenum or lower esophagus jejunum, and mechanical expansion. Various preparation methods can induce different degrees of esophageal lesions, but each has its disadvantages in the actual research. The development of animal models should be based on the experimental purposes and requirements to choose suitable animals and reasonable operation, and take effective measures so as to prevent postoperative complications and lower mortality, improve the utilization rate, and prepare ideal animal models to meet the needs of research. © 2014 Baishideng Publishing Group Inc. All rights reserved.
Li Y.,Community Health Service Center |
Hua Y.,Community Health Service Center |
Zhong N.,Community Health Service Center
Journal of Acupuncture and Tuina Science | Year: 2014
Objective: To observe the clinical efficacy of warm needling plus tuina in treating senile functional constipation.Methods: Ninety elderly patients with functional constipation were randomized into two groups. Forty-six patients in the treatment group were intervened by warm needling plus tuina; the other forty-four patients in the control group were intervened by San Huang tablet.Results: In the treatment group, 19 patients were cured, 24 cases showed improvement, 3 cases failed, and the total effective rate was 93.5%; in the control group, 16 patients were cured, 18 cases showed improvement, 10 cases failed, and the total effective rate was 77.3%. There was a significant difference in comparing the therapeutic efficacy (P<0.05), and the therapeutic efficacy of the treatment group was higher than that of the control group.Conclusion: Warm needling plus tuina can produce a higher therapeutic efficacy than taking San Huang tablet in treating senile functional constipation. © 2014, Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.
Zou C.-L.,Community Health Service Center
Journal of Acupuncture and Tuina Science | Year: 2014
Objective: To observe the effect of warm needling therapy plus acupuncture on third lumbar vertebra transverse process syndrome. Methods: Sixty cases of third lumbar vertebra transverse process syndrome were randomly divided into a warm needling group or an acupuncture group, 30 cases in each group. Cases in the acupuncture group were treated by routine points and needle sticking manipulation, while those in the warm needling group were treated by moxibustion plus needling as same as the acupuncture group. Japanese orthopedic association scores (JOA) in the two groups were observed and compared after 2 treatment courses. Results: After treatment, the JOA score in the warm needling group was higher than that in the acupuncture group (P<0.01); subjective symptom, clinical syndromes, activity of daily living (ADL) in the warm needling group were all higher than those in the acupuncture group (all P<0.05); the score of pain in the warm needling group was significantly lower than that in the acupuncture group (P<0.01); the total effective rate in the warm needling group was 76.7%, versus 70.0% in the acupuncture group, showing a statistically significant difference (P<0.05). Conclusion: Warm needling therapy is more effective than normal acupuncture in treating third lumbar vertebra transverse process syndrome. © 2014 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.
PubMed | Community Health Service Center and Tianjin Medical University
Type: | Journal: Scientific reports | Year: 2016
This study aimed to evaluate whether folic acid supplementation would improve cognitive performance by reducing serum inflammatory cytokine concentrations. This RCT was performed in Tianjin, China. Participants with mild cognitive impairment (MCI) were randomly assigned to the folic acid (400g/day) or conventional treatment groups. Neuropsychological tests were administered, and folate, homocysteine, vitamin B
PubMed | Community Health Service Center, Tianjin Medical University and The First Center Hospital
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016
Metastasis is a major cause of lung cancer-associated mortality. The current study aimed to investigate the effects and mechanisms of TAR (human immunodeficiency virus1) RNA binding protein2 (TARBP2) in the invasion and migration of nonsmall cell lung cancer invitro. The highly metastatic cell clone H1299/M02 was obtained by TARBP2 overexpression. Expression of TARBP2 in H1299/M02 was also downregulated to different levels via small hairpin RNAs (shRNAs). Subsequent to TARBP2 silencing, the proliferation of H1299/M02 cells was predominantly unaffected, while invasion and migration were significantly inhibited. A positive correlation was observed between invasion and migration and the level of TARBP2 silencing invitro. Western blotting and reverse transcriptionquantitative polymerase chain reaction indicated that the protein expression levels of amyloid(A4) precursor protein (APP) and zinc finger protein395 (ZNF395) were upregulated, while expression levels of prometastatic proteins including interleukin (IL)1, IL8, cyclooxygenase (COX)2, matrix metalloproteinase2 (MMP2) and MMP9 were downregulated. Phosphorylation of cJun Nterminal kinase (JNK), signal transducer and activator of transcription3 (STAT3) and protein kinaseB (AKT) were also inhibited. Overexpression of TARBP2 was suggested to be involved in the metastasis of H1299/M02 cells. Silencing of TARBP2 was able to upregulate levels of APP and ZNF395, in addition to inhibiting metastasispromoting cytokines, the JNK/STAT3/AKT pathway and COX2 to attenuate the invasion and migration of cancer cells.