Time filter

Source Type

Barral S.,Columbia University Medical Center and New York Presbyterian Hospital | Barral S.,Columbia University | Vardarajan B.N.,Columbia University Medical Center and New York Presbyterian Hospital | Vardarajan B.N.,Columbia University | And 25 more authors.
Neurobiology of Aging | Year: 2015

Psychotic symptoms are frequent in late-onset Alzheimer's disease (LOAD) patients. Although the risk for psychosis in LOAD is genetically mediated, no genes have been identified. To identify loci potentially containing genetic variants associated with risk of psychosis in LOAD, a total of 263 families from the National Institute of Aging-LOAD cohort were classified into psychotic (LOAD+P, n = 215) and nonpsychotic (LOAD-P, n = 48) families based on the presence/absence of psychosis during the course of LOAD. The LOAD+P families yielded strong evidence of linkage on chromosome 19q13 (two-point [2-pt]logarithm of odds [LOD] = 3.8, rs2285513 and multipoint LOD = 2.7, rs541169). Joint linkage and association in 19q13 region detected strong association with rs2945988 (p = 8.7 × 10-7). Linkage results for the LOAD-P families yielded nonsignificant 19q13 LOD scores. Several 19q13 single-nucleotide polymorphisms generalized the association of LOAD+P in a Caribbean Hispanic (CH) cohort, and the strongest signal was rs10410711 (pmeta = 5.1 × 10-5). A variant located 24 kb upstream of rs10410711 and rs10421862 was strongly associated with LOAD+P (pmeta = 1.0 × 10-5) in a meta-analysis of the CH cohort and an additional non-Hispanic Caucasian dataset. Identified variants rs2945988 and rs10421862 affect brain gene expression levels. Our results suggest that genetic variants in genes on 19q13, some of which are involved in brain development and neurodegeneration, may influence the susceptibility to psychosis in LOAD patients. © 2015 The Authors.


PubMed | University of Washington, Pontifical Catholic University Madre y Maestra, Indiana University, Mayo Medical School and 5 more.
Type: Journal Article | Journal: Neurobiology of aging | Year: 2015

Psychotic symptoms are frequent in late-onset Alzheimers disease (LOAD) patients. Although the risk for psychosis in LOAD is genetically mediated, no genes have been identified. To identify loci potentially containing genetic variants associated with risk of psychosis in LOAD, a total of 263 families from the National Institute of Aging-LOAD cohort were classified into psychotic (LOAD+P, n= 215) and nonpsychotic (LOAD-P, n= 48) families based on the presence/absence of psychosis during the course of LOAD. The LOAD+P families yielded strong evidence of linkage on chromosome 19q13 (two-point [2-pt] logarithm of odds [LOD]= 3.8, rs2285513 and multipoint LOD= 2.7, rs541169). Joint linkage and association in 19q13 region detected strong association with rs2945988 (p= 8.7 10(-7)). Linkage results for the LOAD-P families yielded nonsignificant 19q13 LOD scores. Several 19q13 single-nucleotide polymorphisms generalized the association of LOAD+P in a Caribbean Hispanic (CH) cohort, and the strongest signal was rs10410711 (pmeta= 5.1 10(-5)). A variant located 24 kb upstream of rs10410711 and rs10421862 was strongly associated with LOAD+P (pmeta=1.010(-5)) in a meta-analysis of the CH cohort and an additional non-Hispanic Caucasian dataset. Identified variants rs2945988 and rs10421862 affect brain gene expression levels. Our results suggest that genetic variants in genes on 19q13, some of which are involved in brain development and neurodegeneration, may influence the susceptibility to psychosis in LOAD patients.


Kwee I.,Oncology Institute of Southern Switzerland IOSI | Kwee I.,Dalle Molle Institute for Artificial Intelligence | Rancoita P.M.V.,Oncology Institute of Southern Switzerland IOSI | Rancoita P.M.V.,Dalle Molle Institute for Artificial Intelligence | And 10 more authors.
Haematologica | Year: 2011

MALT lymphomas present common features, but important differences are associated with involvement of specific anatomical sites, many likely contributing to the biology. To test the existence of genetic alterations specific for primary anatomical sites of involvement, genomic profiles obtained with high-density arrays were analyzed in 130 MALT lymphomas across a spectrum of anatomic sites. Trisomies 3 and 18 and del(6q23) occurred at a similar frequency. Instead, gains at 6p appeared significantly more common among MALT lymphomas involving the orbital adnexa. Gastric involvement showed a trend for a higher frequency of 8q gains. In conclusion, MALT lymphomas appear to bear a common set of recurrent unbalanced genomic alterations independent of the anatomical site. This differs from what has been observed for common chromosome translocations. Only a few alterations such as gains at 6p and, possibly, gains at 8q show preferential involvement at specific anatomical sites. ©2011 Ferrata Storti Foundation.


Li M.,Thermo Fisher Scientific | Rai A.J.,Columbia University Medical Center and New York Presbyterian Hospital | Joel DeCastro G.,Columbia University | Zeringer E.,Thermo Fisher Scientific | And 4 more authors.
Methods | Year: 2015

Exosomes are RNA and protein-containing nanovesicles secreted by all cell types and found in abundance in body fluids, including blood, urine and cerebrospinal fluid. These vesicles seem to be a perfect source of biomarkers, as their cargo largely reflects the content of parental cells, and exosomes originating from all organs can be obtained from circulation through minimally invasive or non-invasive means. Here we describe an optimized procedure for exosome isolation and analysis using clinical samples, starting from quick and robust extraction of exosomes with Total exosome isolation reagent, then isolation of RNA followed by qRT-PCR. Effectiveness of this workflow is exemplified by analysis of the miRNA content of exosomes derived from serum samples - obtained from the patients with metastatic prostate cancer, treated prostate cancer patients who have undergone prostatectomy, and control patients without prostate cancer. Three promising exosomal microRNA biomarkers were identified, discriminating these groups: hsa-miR375, hsa-miR21, hsa-miR574. © 2015 Elsevier Inc.


Bao F.,Columbia University Medical Center and New York Presbyterian Hospital | Bhagat G.,Columbia University
Gastrointestinal Endoscopy Clinics of North America | Year: 2012

Small bowel biopsy remains the gold standard for diagnosing celiac disease (CD). Intraepithelial lymphocytosis in the context of villous atrophy is considered a characteristic histologic finding of CD. However, studies have also indicated that the detection of intraepithelial lymphocytosis in the absence of villous atrophy is not specific for CD, having been documented in other small intestinal disorders. This review summarizes key aspects regarding the histopathologic assessment, impact of the site and number of small bowel biopsy samples on diagnosis, old and emerging classifications, and benefit of standardized pathology report in the diagnostic workup of CD. © 2012.


PubMed | Columbia University Medical Center and New York Presbyterian Hospital
Type: Journal Article | Journal: Radiology | Year: 2010

To determine radiation doses from coronary computed tomographic (CT) angiography performed by using a 320-detector row volume scanner and evaluate how the effective dose depends on scan mode and the calculation method used.Radiation doses from coronary CT angiography performed by using a volume scanner were determined by using metal-oxide-semiconductor field-effect transistor detectors positioned in an anthropomorphic phantom physically and radiographically simulating a male or female human. Organ and effective doses were determined for six scan modes, including both 64-row helical and 280-row volume scans. Effective doses were compared with estimates based on the method most commonly used in clinical literature: multiplying dose-length product (DLP) by a general conversion coefficient (0.017 or 0.014 mSv.mGy(-1).cm(-1)), determined from Monte Carlo simulations of chest CT by using single-section scanners and previous tissue-weighting factors.Effective dose was reduced by up to 91% with volume scanning relative to helical scanning, with similar image noise. Effective dose, determined by using International Commission on Radiological Protection publication 103 tissue-weighting factors, was 8.2 mSv, using volume scanning with exposure permitting a wide reconstruction window, 5.8 mSv with optimized exposure and 4.4 mSv for optimized 100-kVp scanning. Estimating effective dose with a chest conversion coefficient resulted in a dose as low as 1.8 mSv, substantially underestimating effective dose for both volume and helical coronary CT angiography.Volume scanning markedly decreases coronary CT angiography radiation doses compared with those at helical scanning. When conversion coefficients are used to estimate effective dose from DLP, they should be appropriate for the scanner and scan mode used and reflect current tissue-weighting factors. (c) RSNA, 2010.


PubMed | Columbia University Medical Center and New York Presbyterian Hospital
Type: Historical Article | Journal: Neurosurgery | Year: 2010

This presentation is a succinct pictorial essay reviewing the history of the Neurological Institute of New York through the succession of its Chairmen of Neurosurgery over the past 100 years.


PubMed | Columbia University Medical Center and New York Presbyterian Hospital
Type: Journal Article | Journal: British journal of haematology | Year: 2011

Three distinct categories of marginal zone lymphomas (MZLs) are currently recognized, principally based on their site of occurrence. They are thought to represent unique entities, but the relationship of one subtype with another is poorly understood. We investigated 17 non-splenic MZLs (seven nodal, 10 extranodal) by gene expression profiling to distinguish between subtypes and determine their cell of origin. Our findings suggest biological inter-relatedness of these entities despite occurrence at different locations and associations with possibly different aetiologies. Furthermore, the expression profiles of non-splenic MZL were similar to memory B cells.


PubMed | Columbia University, Columbia University Medical Center and New York Presbyterian Hospital and Thermo Fisher Scientific
Type: | Journal: Methods (San Diego, Calif.) | Year: 2015

Exosomes are RNA and protein-containing nanovesicles secreted by all cell types and found in abundance in body fluids, including blood, urine and cerebrospinal fluid. These vesicles seem to be a perfect source of biomarkers, as their cargo largely reflects the content of parental cells, and exosomes originating from all organs can be obtained from circulation through minimally invasive or non-invasive means. Here we describe an optimized procedure for exosome isolation and analysis using clinical samples, starting from quick and robust extraction of exosomes with Total exosome isolation reagent, then isolation of RNA followed by qRT-PCR. Effectiveness of this workflow is exemplified by analysis of the miRNA content of exosomes derived from serum samples - obtained from the patients with metastatic prostate cancer, treated prostate cancer patients who have undergone prostatectomy, and control patients without prostate cancer. Three promising exosomal microRNA biomarkers were identified, discriminating these groups: hsa-miR375, hsa-miR21, hsa-miR574.


PubMed | Columbia University Medical Center and New York Presbyterian Hospital and Columbia University
Type: Journal Article | Journal: Journal of cardiovascular computed tomography | Year: 2016

Transcatheter aortic valve replacement (TAVR) is a lifesaving procedure for many patients high risk for surgical aortic valve replacement. The prevalence of chronic kidney disease (CKD) is high in this population, and thus a very low contrast volume (VLCV) computed tomography angiography (CTA) protocol providing comprehensive cardiac and vascular imaging would be valuable.52 patients with severe, symptomatic aortic valve disease, undergoing pre-TAVR CTA assessment from 2013-4 at Columbia University Medical Center were studied, including all 26 patients with CKD (eGFR<30mL/min) who underwent a novel VLCV protocol (20mL of iohexol at 2.5mL/s), and 26 standard-contrast-volume (SCV) protocol patients. Using a 320-slice volumetric scanner, the protocol included ECG-gated volume scanning of the aortic root followed by medium-pitch helical vascular scanning through the femoral arteries. Two experienced cardiologists performed aortic annulus and root measurements. Vascular image quality was assessed by two radiologists using a 4-point scale.VLCV patients had mean (SD) age 866.5, BMI 23.93.4kg/m(2) with 54% men; SCV patients age 838.8, BMI 28.75.3kg/m(2), 65% men. There was excellent intra- and inter-observer agreement for annular and root measurements, and excellent agreement with 3D-transesophageal echocardiographic measurements. Both radiologists found diagnostic-quality vascular imaging in 96% of VLCV and 100% of SCV cases, with excellent inter-observer agreement.This study is the first of its kind to report the feasibility and reproducibility of measurements for a VLCV protocol for comprehensive pre-TAVR CTA. There was excellent agreement of cardiac measurements and almost all studies were diagnostic quality for vascular access assessment.

Loading Columbia University Medical Center and New York Presbyterian Hospital collaborators
Loading Columbia University Medical Center and New York Presbyterian Hospital collaborators