Columbia Institute of Pharmacy

Tekāri, India

Columbia Institute of Pharmacy

Tekāri, India
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Dubey S.,Columbia Institute of Pharmacy
International Journal of PharmTech Research | Year: 2014

Pioglitazone hydrochloride is an oral anti-diabetic agent used in the treatment of type 2 diabetes mellitus and also known as non-insulin dependent diabetes mellitus (NIDDM) or adult onset diabetes. A simple, an accurate and economic, precise and reproducible UV Spectroscopy method has been developed for the estimation of Pioglitazone hydrochloride tablet dosage form and validated by ICH guidelines. The standard (10 μg/mL) was scanned between 200-400 nm and maximum absorption was recorded at 231.5 nm.The assay results were found to be 100.52%. The linearity range of 15-65 μg/ml proved that it obeyed Beer's Law and the correlation coefficient (r2) was found to be 0.9983 at 270 nm with an intercept of 0.0008and a slope of 0.0018 with RSD less than 2% complied ICH. The pH degradation study of API was found to be less at pH 7-12. The force degradation studies of Pioglitazone was done on Stress degradation by hydrolysis under alkaline condition by using 0.1N NaOH was found to be 5.76% for 60min, 9.61% for 90min. Stress degradation by hydrolysis under acidic condition by using 3N HCl and product degradation was found to be11.53% for 60min and 21.15% for 90 min for API. Dry heat induced degradation was done by using 70°C temperature was found to be 1.93 % for API for 48 hrs. Oxidative degradation was done by using hydrogen peroxide and product degradation was found to be 19.23% at 15 min. Photolytic degradation was found to be 9.61 % for 3hrs and 15.38% for 5 hrs for API.

Pandit B.,Columbia Institute of Pharmacy
Current Chemical Biology | Year: 2017

Biodegradable and nontoxic natural biopolymers are commonly being studied as a potential carrier material for novel drug delivery system due to its bio-compatible nature. Hydrogels are hydrophilic cross-linked polymer networks. They are able to imbibe large amounts of water or biological fluids. Guar gum (GG) is a galactomannan. It is obtained from plant Cyamopsis Tetragonolobus Linn. It has wide applications in pharmaceutical formulations, cosmetic, food, textile, paper, explosive, toiletries industries, etc. However, due to uncontrollable rate of viscosity, uncontrollable rate of hydration, instability of its solution for a long time and susceptibility to microbial contamination restricts the use of GG in pharmaceutical industries. To overcome these drawbacks, GG should be modified to hydrogel. GG based hydrogels are widely used in pharmaceutical application due to its changes in physic-chemical properties. GG and its derivatives are used as binders, disintegrate in the formulation of tablets. They are also used as control-released agents in novel drug delivery. The present review has been aimed to give an overview of GG based hydrogels for pharmaceutical applications. © 2017 Bentham Science Publishers.

Paliwal S.R.,Guru Ghasidas University | Paliwal R.,Columbia Institute of Pharmacy | Vyas S.P.,Drug Delivery Research Laboratory
Drug Delivery | Year: 2015

The pH-sensitive liposomes have been extensively used as an alternative to conventional liposomes in effective intracellular delivery of therapeutics/antigen/DNA/diagnostics to various compartments of the target cell. Such liposomes are destabilized under acidic conditions of the endocytotic pathway as they usually contain pH-sensitive lipid components. Therefore, the encapsulated content is delivered into the intracellular bio-environment through destabilization or its fusion with the endosomal membrane. The therapeutic efficacy of pH-sensitive liposomes enables them as biomaterial with commercial utility especially in cancer treatment. In addition, targeting ligands including antibodies can be anchored on the surface of pH-sensitive liposomes to target specific cell surface receptors/antigen present on tumor cells. These vesicles have also been widely explored for antigen delivery and serve as immunological adjuvant to enhance the immune response to antigens. The present review deals with recent research updates on application of pH-sensitive liposomes in chemotherapy/diagnostics/antigen/gene delivery etc. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.

Uprit S.,P.A. College | Kumar Sahu R.,Columbia Institute of Pharmacy | Roy A.,Columbia Institute of Pharmacy | Pare A.,VNS Institute of Pharmacy
Saudi Pharmaceutical Journal | Year: 2013

In the present work attempts have been made to prepare the nanostructured lipid carrier (NLC) gel, by using minoxidil, which is preferably used in case of Alopecia, i.e. baldness pattern as a effective drug. The nine different formulations of Minoxidil-NLC (NLC1-NLC9) were prepared using solid and liquid lipids with Cholesterol and Soya lecithin in different concentrations by the melt dispersion ultrasonication method. Properties of NLC1-NLC9 such as the particle size and its distribution, the scanning electron microscopy (SEM), the drug entrapment efficiency (EE), and the drug release behavior were investigated. The nanoparticulate dispersion was suitably gelled and characterized with respect to drug content, pH, spreadability, rheology, and in vitro release. Safety of the NLC-based gel was assessed using primary skin irritation studies. The formulated NLC3 was spherical in shape, with average particle size of 280. nm, zeta potential of -42.40. mV and entrapment efficiency of 86.09%. Differential Scanning Calorimeter (DSC) measurements revealed that imperfect crystallization occurred in the inner core of the NLC particles. The drug release behavior from the NLC displayed a biphasic drug release pattern with burst release at the initial stage followed by sustained release. These results indicated that the NLC3 is a suitable carrier of minoxidil with improved drug loading capacity and controlled drug release properties. It has been observed that NLC gel produces the gel with good consistency, homogeneity, spreadability and rheological behavior. The developed NLC-based gel showed faster onset and elicited prolonged activity up to 16. h. The present study concluded that the NLC-based gel containing minoxidil dissolved in a mixture of solid lipid and liquid lipid in the nanoparticulate form helped us to attain the objective of faster onset yet prolonged action as evident from in vitro release. © 2012.

Paliwal S.R.,Guru Ghasidas University | Paliwal S.R.,Dr Hari Singh Gour University | Paliwal R.,Dr Hari Singh Gour University | Paliwal R.,Columbia Institute of Pharmacy | And 2 more authors.
Journal of Liposome Research | Year: 2016

Context: Surface-modified pH-sensitive liposomal system may be useful for intracellular delivery of chemotherapeutics. Objective: Achieving site-specific targeting with over-expressed hyaluronic acid (HA) receptors along with using pH sensitive liposome carrier for intracellular drug delivery was the aim of this study. Materials and methods: Stealth HA-targeted pH-sensitive liposomes (SL-pH-HA) were developed and evaluated to achieve effective intracellular delivery of doxorubicin (DOX) vis–a-vis enhanced antitumor activity. Results: The in vitro release studies demonstrated that the release of DOX from SL-pH-HA was pH-dependent, i.e. faster at mildly acidic pH ∼5, compared to physiological pH ∼7.4. SLpH-HA was evaluated for their cytotoxicity potential on CD44 receptor expressing MCF-7 cells. The half maximal inhibitory concentration (IC50) of SL-pH-HA and SL-HA were about 1.9 and 2.5 μM, respectively, after 48 h of incubation. The quantitative uptake study revealed higher localization of targeted liposomes in the receptor positive cells, which was further confirmed by fluorescent microscopy. The antitumor efficacy of the DOX-loaded HA-targeted pH-sensitive liposomes was also verified in a tumor xenograft mouse model. Discussion: DOX was efficiently delivered to the tumor site by active targeting via HA and CD44 receptor interaction. The major side-effect of conventional DOX formulation, i.e. cardiotoxicity was also estimated by measuring serum enzyme levels of LDH and CPK and found to be minimized with developed formulation. Overall, HA targeted pH-sensitive liposomes were significantly more potent than the non-targeted liposomes in cells expressing high levels of CD44. Conclusion: Results strongly implies the promise of such liposomal system as an intracellular drug delivery carrier developed for potential anticancer treatment. © 2015 Taylor & Francis.

Das Kurmi B.,Guru Ghasidas University | Tekchandani P.,Guru Ghasidas University | Paliwal R.,Columbia Institute of Pharmacy | Paliwal S.R.,Guru Ghasidas University
Current Pharmaceutical Design | Year: 2015

Heparin, a well known drug for anticoagulant therapy and prophylaxis of deep vein thrombosis and coronary syndromes, is also involved in numerous pathological processes such as inflammation, immune cell migration, tumor cell metastasis, smooth muscle cell proliferation etc. Though heparin is a clinically used anticoagulant with minimal side effects and drug interactions, its clinical use is limited due to parenteral administration. Alternatively, noninvasive delivery approaches such as oral, nasal, pulmonary or transdermal route are being explored that may deal with problems associated with parenteral heparin without compromising therapeutic benefits. For the successful noninvasive delivery of such a large drug candidate, the biological and biochemical barriers must be overcome to achieve a clinically acceptable therapeutic advantage. Nanocarriers significantly improve the pharmacokinetics and clinical effectiveness of the loaded therapeutics by either protecting them from unfavorable bioenvironment or modifying their release at the target site. Novel carriers such as liposomes, nanoparticles, dendrimers etc. have been developed to improve the bioavailability of heparin through various routes of delivery. Overall, the present review provides complete insight to the research that has been carried out for heparin delivery through various routes. © 2015 Bentham Science Publishers.

Surana A.S.,Columbia Institute of Pharmacy
International Journal of Pharmaceutical Sciences Review and Research | Year: 2010

In recent years scientific and technological advancements have been made in the research and development of oral drug delivery system. The reasons that the oral route achieved such popularity may be in part attributed to its ease of administration. Chewing gum is one of the very popular oral confectionery products. Chewing gum is a combination of a water-insoluble phase, known as gum base (insoluble gum base resin), elastomers, emulsifiers, fillers, waxes, antioxidants, softeners, sweeteners, food colourings, flavoring agents, and in case of medical chewing gum, active substances. It offers various advantages over conventional drug delivery system. The manufacturing process of chewing gum takes from 5-15 min, but longer mixing time may be depending on the texture and function of gum base used. An In-vitro apparatus was specially designed and constructed for release testing of medicated chewing gums. The absorption of active substances through the buccal mucosa can be examined by both In-vitro and In-vivo methods. It was concluded that Chewing gum is an excellent drug delivery system for self-medication as it is convenient and can be administered discreetly without water.

Subudhi B.B.,Siksha ‘O’ Anusandhan University | Singh V.K.,Columbia Institute of Pharmacy
Anti-Cancer Agents in Medicinal Chemistry | Year: 2016

Treatment of brain tumour is a major challenge. This is mainly because of the limited bioavailability of chemotherapeutics in the brain. The major hurdle for brain availability of anticancer agents is the blood brain barrier (BBB). BBB is supposed to protect the brain and maintain homeostasis. It allows vital nutrient for normal brain function and effluxes out foreign toxic substance. Advance in knowledge of bidirectional movement across BBB has allowed development of strategies to enhance brain availability of chemotherapeutics for management of brain tumour. In this review we have focussed on various approaches adopted for enhancing delivery of anticancer agents. We have given a critical analysis of the approaches for further research © 2016 Bentham Science Publishers.

Roy A.,Columbia Institute of Pharmacy | Prasad P.,Columbia Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2013

Mushrooms are known for their nutritional and culinary values and used as medicines by humans for centuries. They are also known for their high quality proteins, vitamins, fibres and thus called as "nutraceuticals". The chemical nature of the bioactive compounds present in this mushroom includes: polysaccharides, lipopolysaccharides, proteins, peptides, glycoproteins, nucleosides, lipids and their derivatives. Mushrooms are one of the prominent functional food, rich in protein but also as a source of biologically active compounds of medicinal values which include medicine/dietary supplements for anticancer, antiviral, hypotensive, immunomodulatory agent and many others. Pleurotus ostreatus possesses many medicinal properties and also used as health promoter. It is gaining more importance than any other mushrooms as therapeutic agent and for health benefits. In the present paper the medicinal properties of Pleurotus ostreatus is being discussed. © RJPT.

Ahirwar K.,Columbia Institute of Pharmacy | Jain S.K.,Columbia Institute of Pharmacy
International Journal of Phytomedicine | Year: 2011

The electrochemical behaviour of the anticancer herbal drug emodin hydroxyanthraquinone present in Aloe vera leaves has a specific in vitro and in vivo antineuroectodermal tumor activity. The compound does not inhibit the proliferation of normal fibroblasts n or that of hemopoietic progenitor cells. The cytotoxicity mechanism consists of the induction of apoptosis, whereas the selectivity against neuroectodermal tumor cells is founded on a specific energy-dependent pathway of drug incorporation. Natural compounds that have traditionally been used to treat a variety of diseases for hundreds of years (1, 2, 3). We assayed only those natural compounds that have already been proven to be nontoxic, and we evaluated their efficacy against highly malignant tumors that are not normally included in the classical screening assays.

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