Dubey S.,Columbia Institute of Pharmacy
International Journal of PharmTech Research | Year: 2014
Pioglitazone hydrochloride is an oral anti-diabetic agent used in the treatment of type 2 diabetes mellitus and also known as non-insulin dependent diabetes mellitus (NIDDM) or adult onset diabetes. A simple, an accurate and economic, precise and reproducible UV Spectroscopy method has been developed for the estimation of Pioglitazone hydrochloride tablet dosage form and validated by ICH guidelines. The standard (10 μg/mL) was scanned between 200-400 nm and maximum absorption was recorded at 231.5 nm.The assay results were found to be 100.52%. The linearity range of 15-65 μg/ml proved that it obeyed Beer's Law and the correlation coefficient (r2) was found to be 0.9983 at 270 nm with an intercept of 0.0008and a slope of 0.0018 with RSD less than 2% complied ICH. The pH degradation study of API was found to be less at pH 7-12. The force degradation studies of Pioglitazone was done on Stress degradation by hydrolysis under alkaline condition by using 0.1N NaOH was found to be 5.76% for 60min, 9.61% for 90min. Stress degradation by hydrolysis under acidic condition by using 3N HCl and product degradation was found to be11.53% for 60min and 21.15% for 90 min for API. Dry heat induced degradation was done by using 70°C temperature was found to be 1.93 % for API for 48 hrs. Oxidative degradation was done by using hydrogen peroxide and product degradation was found to be 19.23% at 15 min. Photolytic degradation was found to be 9.61 % for 3hrs and 15.38% for 5 hrs for API.
Vyas A.,University Institute of Pharmacy |
Shukla S.S.,Columbia Institute of Pharmacy |
Pandey R.,Columbia Institute of Pharmacy |
Jain V.,University Institute of Pharmacy |
Gidwani B.,University Institute of Pharmacy
Asian Journal of Plant Sciences | Year: 2012
Use of herbs to remedy many ailments is known since ages. Indeed, the use of herbs is not only natural way to remedy diseases but also without harmful side effects. Chervil (Anthriscus cerefolium) is a delicate annual herb, full of nutritional value. It is a common plant genus of the family Apiaceae. It comprises 12 species, some of which are considered as noxious weeds. The genus grows in meadows and verges on slightly wet porous soils. This plant finds wide use in treatment of various diseases. Also, it is considered safe to use this herb by the people throughout the world. It is also called 'gourmet's parsley'. The active constituents of chervil are volatile oil, flavonoids and coumarins, other constituents present are methyl chavicol (estragole) and hendecane (undecane). This herb is used in kitchen for due to its nutritional aspect. The entire herb is advantageous, miraculous and nutritious in nature. ©2012 Asian Network for Scientific Information.
Surana A.S.,Columbia Institute of Pharmacy
International Journal of Pharmaceutical Sciences Review and Research | Year: 2010
In recent years scientific and technological advancements have been made in the research and development of oral drug delivery system. The reasons that the oral route achieved such popularity may be in part attributed to its ease of administration. Chewing gum is one of the very popular oral confectionery products. Chewing gum is a combination of a water-insoluble phase, known as gum base (insoluble gum base resin), elastomers, emulsifiers, fillers, waxes, antioxidants, softeners, sweeteners, food colourings, flavoring agents, and in case of medical chewing gum, active substances. It offers various advantages over conventional drug delivery system. The manufacturing process of chewing gum takes from 5-15 min, but longer mixing time may be depending on the texture and function of gum base used. An In-vitro apparatus was specially designed and constructed for release testing of medicated chewing gums. The absorption of active substances through the buccal mucosa can be examined by both In-vitro and In-vivo methods. It was concluded that Chewing gum is an excellent drug delivery system for self-medication as it is convenient and can be administered discreetly without water.
Subudhi B.B.,Siksha ‘O’ Anusandhan University |
Singh V.K.,Columbia Institute of Pharmacy
Anti-Cancer Agents in Medicinal Chemistry | Year: 2016
Treatment of brain tumour is a major challenge. This is mainly because of the limited bioavailability of chemotherapeutics in the brain. The major hurdle for brain availability of anticancer agents is the blood brain barrier (BBB). BBB is supposed to protect the brain and maintain homeostasis. It allows vital nutrient for normal brain function and effluxes out foreign toxic substance. Advance in knowledge of bidirectional movement across BBB has allowed development of strategies to enhance brain availability of chemotherapeutics for management of brain tumour. In this review we have focussed on various approaches adopted for enhancing delivery of anticancer agents. We have given a critical analysis of the approaches for further research © 2016 Bentham Science Publishers.
Roy A.,Columbia Institute of Pharmacy |
Prasad P.,Columbia Institute of Pharmacy
Research Journal of Pharmacy and Technology | Year: 2013
Mushrooms are known for their nutritional and culinary values and used as medicines by humans for centuries. They are also known for their high quality proteins, vitamins, fibres and thus called as "nutraceuticals". The chemical nature of the bioactive compounds present in this mushroom includes: polysaccharides, lipopolysaccharides, proteins, peptides, glycoproteins, nucleosides, lipids and their derivatives. Mushrooms are one of the prominent functional food, rich in protein but also as a source of biologically active compounds of medicinal values which include medicine/dietary supplements for anticancer, antiviral, hypotensive, immunomodulatory agent and many others. Pleurotus ostreatus possesses many medicinal properties and also used as health promoter. It is gaining more importance than any other mushrooms as therapeutic agent and for health benefits. In the present paper the medicinal properties of Pleurotus ostreatus is being discussed. © RJPT.
Dubey P.,Columbia Institute of Pharmacy |
Gidwani B.,Pandit Ravishankar Shukla University |
Pandey R.,Columbia Institute of Pharmacy |
Shukla S.S.,Columbia Institute of Pharmacy
Artificial Cells, Nanomedicine and Biotechnology | Year: 2016
The purpose of this study was to develop PEGylated nanoparticles of bendamustine (BM) to improve therapeutic efficiency of drug and reduce the side-effects. The nanoparticles were prepared by a modified diffusion–emulsification method. The particle size and zeta potential of optimized BM-loaded PEGylated NPs were found to be 256 nm and −29.1 mV. The in vitro release showed biphasic behavior, with initial burst release followed by slow sustained delivery. The anti-tumor activity was determined using the A- 549 cell line, by the MTT assay. The stability study revealed that the nanoparticles prepared were stable for 3 months at both 25°C and 4°C. © 2015 Informa Healthcare USA, Inc.
Singh V.K.,Columbia Institute of Pharmacy |
Subudhi B.B.,Siksha ‘O’ Anusandhan University
Medicinal Chemistry Research | Year: 2015
Poor permeability of methotrexate across blood brain barrier limits its scope for application against brain cancer. Glutamine is transported to brain actively by multiple amino acid transporters and protects against complications of chemotherapy. To use glutamine as a carrier, a reversible conjugate of methotrexate was developed with structural features of glutamine. This was stable and released methotrexate effectively. The brain uptake was enhanced significantly. © 2014 Springer Science+Business Media New York.
Ahirwar K.,Columbia Institute of Pharmacy |
Jain S.K.,Columbia Institute of Pharmacy
International Journal of Phytomedicine | Year: 2011
The electrochemical behaviour of the anticancer herbal drug emodin hydroxyanthraquinone present in Aloe vera leaves has a specific in vitro and in vivo antineuroectodermal tumor activity. The compound does not inhibit the proliferation of normal fibroblasts n or that of hemopoietic progenitor cells. The cytotoxicity mechanism consists of the induction of apoptosis, whereas the selectivity against neuroectodermal tumor cells is founded on a specific energy-dependent pathway of drug incorporation. Natural compounds that have traditionally been used to treat a variety of diseases for hundreds of years (1, 2, 3). We assayed only those natural compounds that have already been proven to be nontoxic, and we evaluated their efficacy against highly malignant tumors that are not normally included in the classical screening assays.
Satapathy T.,Columbia Institute of Pharmacy |
Panda P.K.,Utkal University
Research Journal of Pharmacy and Technology | Year: 2013
Gastro esophageal reflux disease (GERD) is a common chronic disorder that is associated with a huge economic burden in the many countries and significantly decreased quality of life. Nearly all epidemiologic studies have found an association between increasing body mass index (BMI) and symptoms of gastro esophageal reflux disease (GERD). Changes in gastro esophageal anatomy and physiology caused by obesity may explain the association. These include an increased prevalence of esophageal motor disorders, diminished lower esophageal sphincter (LES) pressure, the development of a hiatal hernia, and increased intragastric pressure. GERD can lead to complications that include esophageal stricture and esophageal adenocarcinoma. Multiple challenges are associated with GERD treatment. First, lack of symptoms does not correlate with the absence of or the healing of esophageal lesions. Second, proton pump inhibitors, the current standard of care for GERD, are ineffective for the majority of GERD patients who have nonerosive disease. Investigations and technical advances have enhanced our understanding and management of gastro esophageal reflux disease. The recognition of the prevalence and importance of patients with endoscopy-negative reflux disease as well as those refractory to proton pump inhibitor therapy have led to an increasing need for objective tests of esophageal reflux. This review focuses on the various multicultural issues in the epidemiology, pathophysiology, diagnosis, and treatment of GERD. © RJPT All right reserved.
PubMed | Columbia Institute of Pharmacy, Indian Institute of Integrative Medicine, Sarguja University and Pandit Ravishankar Shukla University
Type: Journal Article | Journal: Journal of ethnopharmacology | Year: 2014
The plant Diospyros melanoxylon (Roxb) belongs to the family Ebenaceae that is native to India and Sri-lanka. This is a medium-sized tree, reaching a height of 15 m and is well known for its beedi making leaves throughout the world. The purpose of the present study is to assess the effect of Diospyros melanoxylon leaves petroleum ether extract on blood glucose level, lipid level, insulin level, body weight, water and food intake in Streptozotocin (STZ) induced diabetic rats.Two different doses of extract AK001 (250 mg/kg) and AK002 (500 mg/kg) of Diospyros melanoxylon leaves were taken to evaluate different activities. The animals were divided into five groups namely normal control, diabetic control, reference group, AK001 and AK002 each containing six animals for in-vivo study. In-vitro study for antiadipogen activity was performed on 3T3-L1 cell line.The extract showed dose dependent fall in Fasting Glucose Level (FSG) in experimental diabetic animals with significant reduction in food and water intake and increase in body weight. The extract exhibited hypocholesterolemic and hypotriglyceridemic effects while increased level of HDL in diabetes induced rats. In-vitro activity showed more than 75% viability of cells and significant inhibition in differentiated cells as compared to non-differentiate cells in 3T3-L1 cell line. The extract exhibited the concentration-dependent inhibitory effect with an IC50 value of 689.22 g/ml.The extract exhibited significant results for antiadipogenic, antidiabetic and hypolipidemic activity both in-vivo and in-vitro and it may prove to be effective for the treatment of both types of diabetes, i.e. Insulin Dependent Diabetes Mellitus (IDDM) and Noninsulin Dependent Diabetes Mellitus (NIDDM).