Colombian Corporation of Agricultural Research

Bogotá, Colombia

Colombian Corporation of Agricultural Research

Bogotá, Colombia
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Martinez R.,Colombian Corporation of Agricultural Research | Bejarano D.,Colombian Corporation of Agricultural Research | Gomez Y.,Colombian Corporation of Agricultural Research | Dasoneville R.,Genes Diffusion | And 4 more authors.
Genetics and Molecular Biology | Year: 2017

Genotypic and phenotypic data of 1,562 animals were analyzed to find genomic regions that potentially influence the birth weight (BW), weaning weight at seven months of age (WW) and yearling weight (YW) of Colombian Brahman cattle, with genotyping conducted using Illumina Bead chip array with 74,669 SNPs. A Single Step Genomic BLUP (ssGBLP), approach was used to estimate the proportion of variance explained by each marker. Multiple regions scattered across the genome were found to influence weights at different ages, also dependent on the trait component (direct or maternal). The most interesting regions were connected to previously identified QTLs and genes, such as ADAMTSL3, CAPN2, CAPN2, FABP6, ZEB2 influencing growth and weight traits. The identified regions will contribute to the development and refinement of genomic selection programs for Zebu Brahman cattle in Colombia. © 2017, Sociedade Brasileira de Genética.


Chaparro M.L.,Colombian Corporation of Agricultural Research | Cespedes E.,Colombian Corporation of Agricultural Research | Cruz M.,Colombian Corporation of Agricultural Research | Castillo-Saldarriaga C.R.,Colombian Corporation of Agricultural Research | Gomez-Alvarez M.I.,Colombian Corporation of Agricultural Research
Revista Mexicana de Ingeniera Quimica | Year: 2017

Meyerozyma guilliermondii strain Lv196 has been identified as a native Colombian yeast with a high probiotic potential increasing rumen microbial efficiency at in vitro assays when a ruminant digestion system is simulated. Often, these types of products are supplied in a granulated form to facilitate its dosage to ruminants as cows, sheep, among others. To produce granulated bioproducts based on microorganisms, it is required to select suitable drying conditions, protectants and the adequate drying operation, decisive factors to guarantee cell viability and stability during storage. Most granulated products are dried through fluidized bed drying process reducing investment and maintenance costs, and improving mass and heat transfer decreasing operation time and the risk of product overheating. Therefore, in this work a selection of drying protectants through decision matrix was conducted based on parameters as compatibility with Lv196 and cost of protectants. The best two drying protectants were used as functional coadjuvants in the formulation of two granulated prototypes. Drying temperature, chamber pressure and the two prototypes were evaluated in an experimental design to select adequate conditions for a fluidized bed drying process. Finally, the stability of two dried granulated prototypes using selected conditions was determined. Prototype PF2 dried at 45°C and 0.5 bar during 30 min had a final moisture of 7.84 ± 0.01% and operation yield of 98.54 ± 0.11% guaranteeing a loss of viability lower than 2.09 ± 0.01% after 15 months of storage at room temperature. © 2017, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.


Martinez R.A.,Colombian Corporation of Agricultural Research | Dassonneville R.,Genes Diffusion | Bejarano D.,Colombian Corporation of Agricultural Research | Jimenez A.,National Association of Zebu Brahman Breeders ASOCEBU | And 3 more authors.
Journal of Animal Science | Year: 2016

Covariance components and genetic parameters were estimated for birth weight (BiW); adjusted weights at 4, 7, 12, and 18 mo; and ADG between 0 and 4 mo, between 4 and 7 mo, between 7 and 12 mo, and between 12 and 18 mo. Additionally, reproductive traits, calving interval, and age at first calving were analyzed, together with traits measured by ultrasound: loin eye area, deep fat mean, back fat, and rump fat. Analyses were performed using an animal model, considering the fixed effects of the farm (n = 37), year and month of birth, sex, calving number (1 to 7), season (dry and rainy seasons), region (North Coast, Andean Region, and Oriental Savannas), and conception (natural mating or AI), whereas the age of the cows at calving was considered a polynomial covariate with linear and quadratic effects. Three different models were used to find the one with the best fit for each trait: a single-trait model with an additive direct genetic effect, a single-trait model with additive direct and maternal genetic effects, and finally, a multitrait model with an additive direct genetic effect. For the growth traits, the heritability was between 0.24 and 0.47, with the lowest value for weight at 7 mo and the greatest value for BiW, and the maternal heritability was found to be between 0.15 and 0.21 but did not decrease later on. The correlation between direct and maternal effects was high and negative (−0.59 to −0.76). With ultrasound traits, a model with only direct effects was used. The heritability was between 0.13 and 0.28 for back fat and loin eye area, respectively. The heritabilities for deep fat mean and rump fat were similar, being 0.19 and 0.21, respectively. The reproductive traits showed high residual variance. In particular, the heritability of calving interval was low (0.06). The results showed that the growth traits have an important genetic component, which is a favorable indicator for obtaining improvement progress in the zebu Brahman breed for beef production in tropical regions of Colombia. The maternal effects influenced the weight performance from 4 to 18 mo and should be taken into account in genetic analyses. In general, the direct heritability of medium magnitude estimated for growth and traits measured by ultrasound suggests that individual selection for these traits will be effective. © 2016 American Society of Animal Science. All rights reserved.


Zapata J.,Colombian Corporation of Agricultural Research | Acosta C.,Colombian Corporation of Agricultural Research | Diaz A.,Colombian Corporation of Agricultural Research | Villamizar L.,Colombian Corporation of Agricultural Research | Cotes A.M.,Colombian Corporation of Agricultural Research
Acta Horticulturae | Year: 2011

Antagonistic microorganisms are affected by abiotic factors such as pH, temperature UVB radiation and A w when they are applied to the phyllosphere or undergo a formulation processes. However, the selection and design of a correct formulation and manufacturing process, could contribute to solve this problem. In this sense, three isolates of Pichia onychis, Lv027, Lv297 and Lv315 and three isolates of Rhodotorula glutinis, Lv316, Lv317 and Lv318 were characterized by determining the effect of pH, temperature, UVB radiation and A w on growth. The biocontrol activity against Botrytis cinerea in blackberry flowers and cells stability under storage conditions were measured when biomass was dried or suspended in an isotonic solution. A w and UVB radiation were the most limiting factors, when A w was reduced to 0.94 yeast growth reduction of 80% was observed. The growth of P. onychis strains was inhibited completely by UVB, while R. glutinis strains showed a growth inhibition between 16 and 42%. Temperatures between 5 and 37°C and pH between 3 and 9 did not affect yeasts growth. The highest stability was obtained for all yeasts, when cells were suspended in the isotonic solution. All evaluated yeasts reduced the incidence of B. cinerea between 11 and 81% in comparison with the control (90% incidence). However, isolate Lv316 of R. glutinis was selected to continue with a biopesticide development process as an aqueous formulation due to its high biocontrol activity (disease incidence of 18%) and tolerance to the UVB radiation.


Martinez R.,Colombian Corporation of Agricultural Research | Rocha J.F.,Colombian Corporation of Agricultural Research | Bejarano D.,Colombian Corporation of Agricultural Research | Gomez Y.,Colombian Corporation of Agricultural Research | And 2 more authors.
Genetics and Molecular Research | Year: 2016

Colombian creole cattle have important adaptation traits related to heat tolerance and reproductive and productive efficiency. Romosinuano (ROMO) and Blanco Orejinegro (BON) are the most common breeds used by Colombian cattle breeders. Growth traits are of prime importance in these animals, which are mainly raised for beef production. Genes encoding growth hormone, growth hormone receptor, homeobox protein, insulin growth factor binding protein 3, leptin, and myostatin have been associated with physiological growth pathways in cattle and other species. We therefore aimed to identify single nucleotide polymorphisms (SNPs) within these genes in ROMO, BON, and Zebu cattle. DNA regions of these genes were sequenced in 386 animals; 47 new SNPs were found, of which 14 were located in the exonic regions, thereby changing the protein sequence. An association of SNPs with weaning weight (WW), daily weight gain at weaning (DWG), and weight at 16 months (W16M) traits was deduced. The genetic analysis revealed several SNPs related to these traits. The SNP GhRE06.2 had a significant association with WW and the SNP Lep03.4 was highly associated with DWG and W16M. Other polymorphisms were significantly associated with WW and DWG, although they did not surpass the Bonferroni significance threshold. The new mutations identified may indicate important points of genetic control in the DNA that could be responsible for changes in the expression of the analyzed traits. These SNPs might be used in future breeding programs to improve the productive performance of cattle in beef farms. © 2016 The Authors.


PubMed | Colombian Corporation of Agricultural Research
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

Colombian creole cattle have important adaptation traits related to heat tolerance and reproductive and productive efficiency. Romosinuano (ROMO) and Blanco Orejinegro (BON) are the most common breeds used by Colombian cattle breeders. Growth traits are of prime importance in these animals, which are mainly raised for beef production. Genes encoding growth hormone, growth hormone receptor, homeobox protein, insulin growth factor binding protein 3, leptin, and myostatin have been associated with physiological growth pathways in cattle and other species. We therefore aimed to identify single nucleotide polymorphisms (SNPs) within these genes in ROMO, BON, and Zebu cattle. DNA regions of these genes were sequenced in 386 animals; 47 new SNPs were found, of which 14 were located in the exonic regions, thereby changing the protein sequence. An association of SNPs with weaning weight (WW), daily weight gain at weaning (DWG), and weight at 16 months (W16M) traits was deduced. The genetic analysis revealed several SNPs related to these traits. The SNP GhRE06.2 had a significant association with WW and the SNP Lep03.4 was highly associated with DWG and W16M. Other polymorphisms were significantly associated with WW and DWG, although they did not surpass the Bonferroni significance threshold. The new mutations identified may indicate important points of genetic control in the DNA that could be responsible for changes in the expression of the analyzed traits. These SNPs might be used in future breeding programs to improve the productive performance of cattle in beef farms.

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