Li A.-L.,College of Agriculture Science and Education |
Meng X.-C.,College of Agriculture Science and Education |
Duan C.-C.,College of Agriculture Science and Education |
Huo G.-C.,College of Agriculture Science and Education |
And 2 more authors.
Biological and Pharmaceutical Bulletin | Year: 2013
The present study was planned to explore the effect of Lactobacillus (L.) acidophilus on the T helper-17 (Th17) immune response in a mouse model of β-lactoglobulin (β-lg) allergy. Bovine β-lg sensitised BALB/c mice were orally administered with different doses of heat-killed L. acidophilus (low, 5×107 colony forming unit (CFU); medium, 5×10 8 CFU; high, 5×109 CFU) in 200 μL of phosphate buffered saline (PBS) three times a week, starting from 1 week before β-lg sensitisation for 4 weeks. After the allergen challenge, the numbers of blood eosinophils and neutrophils were examined by light microscope; the levels of cytokine (interleukin (IL)-12, IL-4, tumor growth factor (TGF)-β, IL-10, IL-6 and IL-17A), total immunoglobulin E (IgE) and β-lg-specific IgE contents in the serum were measured with enzyme-linked immunosorbent assay (ELISA); The mRNA expression levels of TGF-β, IL-17A, CD25, Foxp3, retinoic acid-related orphan receptor γt (RORγt) and IL-10 were analyzed using real-time polymerase chain reaction (PCR). The results showed that oral administration of L. acidophilus suppressed hypersensitivity responses, attenuated the numbers of inflammatory cells and inhibited IgE production. We found up-regulation of TGF-β and down-regulation of IL-17A in the serum of L. acidophilus-treated group, along with IL-6 levels was significantly decreased than that of the allergy group (p<0.05). Moreover, the mRNA expression levels of CD25, forkhead box P3 and TGF-β were significantly higher in the spleen of L. acidophilus-treated group, while the mRNA expression levels of IL-17A, RORγt and IL-10 were significantly lower than that in the allergy group (p<0.05). In conclusion, the suppression of major allergic symptoms by oral administration of L.acidophilus was probably due to improve the regulatory T (Treg)/Th17 balance and inhibit the IL-6 production. © 2013 The Pharmaceutical Society of Japan.
Brathwaite C.,University of the West Indies |
Bailey J.,College of Agriculture Science and Education
Tropical Agriculture | Year: 2016
This paper focuses on the marketing and consumption of breadfruit for niche markets. It highlights the fact that the evolving eating habits of contemporary consumers is an opportunity for new business in the food and agriculture industry. In particular, the trend of snacking on the go, rather than consuming large meals at specific times of the day, has created the demand for new products and services. This new market niche of snackers presents an opportunity for the breadfruit because of its versatility and novelty. This paper reviews the trends in the snack market as well as the ways in which breadfruit can be used to produce new products for this market. It underscores the fact that the Global Snack Market has been estimated at over $300 Billion US dollars annually, and highlights a small pilot study which indicates that there is in fact room for the breadfruit snack, specifically the breadfruit chip. It ends with suggested areas for further research.
Sarkodie P.A.,College of Agriculture Science and Education
Chemistry | Year: 2015
Learning chemical concepts at the submicroscopic and symbolic levels has been identified as a difficult task for science students. Studies have shown that IUPAC nomenclature of organic compounds, which is at the symbolic level of learning chemical concepts, is a difficult concept when it comes to students' learning. The current study involved a pre-service teacher and 60 high school students and investigated how students could improve upon their performance in naming and writing of structural formulae of hydrocarbons with the aid of ball-and-stick models. The pre-service teacher and the students were purposively selected to participate in the study. After seven weeks of teaching and learning of IUPAC naming and writing of structural formulae of hydrocarbons where the students physically manipulated the models, it was found that the students' performance and attitude improved after the intervention. It is therefore recommended that science educators should continuously use already existing and newly developed models in teaching chemical concepts to help students to actively conceptualise such concepts at the symbolic level.
Goo Y.-M.,Sancheong Oriental Medicinal Herb Institute |
Han E.-H.,Gyeongnam National University of Science and Technology |
Jeong J.C.,Korea Research Institute of Bioscience and Biotechnology |
Kwak S.-S.,Korea Research Institute of Bioscience and Biotechnology |
And 4 more authors.
Comptes Rendus - Biologies | Year: 2015
In a previous study, we have evidenced that the overexpression of the IbOr gene isolated from sweet potato conferred a tolerance activity against salinity and methyl viologen (MV) treatment in transgenic sweet potato calli along with an enhanced carotenoid content. In this study, to further examine the function of the IbOr gene in heterologous organism, we transformed the IbOr gene into potato under the direction of SWPA2 promoter, a strong inducible promoter upon treatment with various environmental stresses. Consistently with ourprevious study of sweet potato calli, the level of total carotenoid was elevated up to 2.7-fold (38.1 μgg 1DW) compared to the non-transgenic control, Atlantic cultivar. However, the composition of carotenoid was not influenced by the overexpression of the IbOr gene since only pre-existing carotenoids in the non-transgenic control including violaxanthin, lutien and β-carotene were elevated at a similar level of total carotenoids. In general, the transcript levels for most of carotenogenesis-related genes were elevated in transgenic tuber, whereas they remained at similar levels in transgenic leaf tissues compared to those of non-transgenic controls. The increased levels of carotenoid content in the leaf or tuber tissue of transgenic lines were correlated with the enhanced tolerance activity against salt-or MV-mediated oxidative stresses and DPPH radical-scavenging activity. Our preliminary results suggest that further investigation is required for the development of a crop tolerant to salinity and other environmental stresses through the overexpression of the IbOr gene. © 2014 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.
PubMed | College of Agriculture Science and Education and Jiangnan University
Type: Journal Article | Journal: Journal of food science and technology | Year: 2016
In this study, the effect of gallic acid on mechanical and water barrier properties of zein-oleic acid 0-4 % composite films was investigated. Molecular weight distribution analysis was carried out to confirm gallic acid induced cross linking through change in molecular weight in fraction containing zein proteins. Results revealed that gallic acid treatment increased tensile strength from 17.9MPa to 26.0MPa, decreased water vapour permeability from 0.60 (gmmm(-2)h(-1) kPa(-1)) to 0.41 (gmmm(-2)h(-1) kPa(-1)), increased solubility from 6.3% to 10.2% and marginally increased elongation at break from 3.7% to 4.2% in zein films only. However, gallic acid treatment in zein-oleic composite films did not significantly influence mechanical and water barrier properties and in most instances irrespective of oleic acid concentration, the properties were negatively affected. Results from scanning electron microscopy showed that both gallic acid treated and untreated zein films and composite films containing 3% oleic acid had a compact and homogeneous structure while those containing 4% oleic acid had inhomogeneous structure. The findings have demonstrated that gallic acid treatment can significantly improve mechanical and water barrier properties especially in zein films only as opposed to when used in composite films using zein and oleic acid.
Nyadanu D.,College of Agriculture Science and Education |
Lowor S.T.,Cocoa Research Institute of Ghana
Genetic Resources and Crop Evolution | Year: 2014
Ghana is endowed with a lot of indigenous leaf and fruit vegetables which are nutritious and are used in the treatment of many ailments. However, current changes in food habits in both rural and urban households have led to overdependence on energy-rich but nutrient poor staple crops. Most of these non-indigenous staple crops are not adapted to local conditions in Ghana as compared to indigenous vegetables making their production and consumption expensive and therefore worsening malnutrition and food insecurity issues. To promote consumption of indigenous vegetables and conservation of their genetic resources in Ghana, proximate, mineral and vitamins composition and medicinal properties of five indigenous leaf (Amaranthus cruentus, Corchorus olitorius, Solanum macrocarpon, Xanthosoma sagittifolium, Adasonia digitata) and three fruit vegetables (Solanum torvum, Solanum aethiopicum, Solanum macrocarpon) vegetables were evaluated using recommended methodologies. Proximate, mineral and vitamins composition varied significantly among the species analyzed. Nutritional composition of indigenous leaf and fruit vegetables were compared with that of exotic vegetables and significant differences were noted. In general, proteins, carbohydrates, dietary fibers, potassium, calcium, magnesium, phosphorus, Vitamin A, Vitamin C and Vitamin E were significantly higher in indigenous vegetables than their exotic counterparts. The results highlight nutritional importance of indigenous vegetables and the need to promote their consumption and safeguard their genetic resources. For further promotion, some urgent research directions are suggested. © 2014 Springer Science+Business Media Dordrecht.
PubMed | Southwest UniversityChongqing, College of Agriculture Science and Education, Southwest University and Purdue University
Type: | Journal: Frontiers in plant science | Year: 2016
Flavonoids, the compounds that impart color to fruits, flowers, and seeds, are the most widespread secondary metabolites in plants. However, a systematic analysis of these loci has not been performed in Brassicaceae. In this study, we isolated 649 nucleotide sequences related to flavonoid biosynthesis, i.e., the
Li A.L.,College of Agriculture Science and Education
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011
To analyse the effect of 7 Lactobacillus species on proliferation and cytokine production of primary lymphocytes, and explored the effect on the Th1/Th2 cells balance. The mouse spleen cells were stimulated with active/heat-killed Lactobacillus strains at different species and concentrations. After be cultured with bacteria 60 hours, the proliferation of splenocytes were tested by MTT colorimetry, and IL-12, IFN-γ, IL-4, IL-10 and TGF-β were detected by ELISA. (1) Both alive and heat-killed Lactobacillus species could accelerate the proliferation of spleen cells in a dose-dependent manner (P<0.05), the immuneological activity of heat-killed L.fermentum and L.acidophilu were not inferior to the live strains, especially when treated at higher dose(10(7);CFU/mL, bacteria: cell ratio of 10:1). Furthermore, these two heat-killed bacterial strains suppressed IL-4, IL-10 and TGF-β production by stimulating IL-12 and IFN-γ production, and their IFN-γ/IL-4 value which represent the Th1/Th2 balance were higher than ConA control group significantly(P<0.05). The results indicate that Lactobacillus strains may differ in their respective ability to improve Th1/Th2 cells balance toward Th1 dominance via increasing the secretion ratio of IFN-γ/IL-4 (Th1/Th2) by splenocytes.
Liu Z.,College of Agriculture Science and Education
BioTechnology: An Indian Journal | Year: 2014
Baseball is a worldwide sports event; its high ornamental and competitiveness charm the people. And baseball game is also a game relies on apparatus, therefore bat some physical properties will have great effects on batters' abilities. This paper targeted market two kinds of textures (allwood and all aluminum) bats hitting effects, it makes deeply research from the two aspects. By deeply researching and analysis of bat hitting process and establishing physical model, it gets bat maximum delivery speed expression, therefore it gets aluminum bat maximum delivery speed is obviously larger than wood bat. Aluminum bat speed change curve is more smoothly than wood bat, which means hitting point position influences on hitting speed is not obvious so that cannot reflect batters' levels. By establishing bat elastic beam model, through analyzing, it gets wood bat and aluminum bat force status. © 2014 Trade Science Inc. - INDIA.
Li A.,College of Agriculture Science and Education
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2012
We investigated the correlation between the in vitro immune profiling of 5 lactobacilli strains and their in vivo protective effect in a mouse beta-lactoglobulin (BLG) allergy model for selecting the candidate strains with potential anti-allergy activity. In vitro immunomodulation was assessed by measuring interleukin (IL)-4 and interferon gamma (IFN-gamma) release by primary lymphocytes stimulated with 5 active/heat-killed lactobacilli. A mice model of beta-lactoglobulin allergy was then used to evaluate the alleviating allergy capacity of the same set of strains. The rats were randomly divided into blank group, BLG allergy group and different lactobacilli strains group. The total IgE and BLG-specific IgE contents in the serum of rats were measured with ELISA. Splenic lymphocytes were isolated and cultured in vitro, the levels of Thl/Th2 type cytokine were detected by ELISA. Protection of BLG-induced allergy was strain-specific. The strains displaying an in vitro capacity to induce higher levels of the Thl type cytokine (IFN-gamma) and lower levels of the Th2 type cytokine (IL-4), significantly decreased the levels of total IgE and BLG-IgE in allergic rat serum (P<0.05). In contrast, strains leading to a low IFN-gamma/IL-4 cytokine ratio could not significantly attenuate allergic symptoms. We could predict the in vivo protective capacity of the studied lactobacilli strains based on the cytokine profile established in vitro. Oral consumption of specific strain may be effective in preventing and alleviating BLG allergic symptoms by the improvement of the Th1/Th2 cell balance toward Th1 dominance, and the inhibition of IgE production.