Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture

Ningbo, China

Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture

Ningbo, China
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Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Zhang J.,Ningbo University | Tu Q.,Zhejiang University | And 4 more authors.
Chemosphere | Year: 2017

Anthropogenic perturbations impose negative effects on coastal ecosystems, such as increasing levels of eutrophication. Given the biogeochemical significance of microorganisms, understanding the processes and mechanisms underlying their spatial distribution under changing environmental conditions is critical. To address this question, we examined how coastal bacterioplankton communities respond to increasing eutrophication levels created by anthropogenic perturbations. The results showed that the magnitude of changes in the bacterioplankton community compositions (BCCs) and the importance of deterministic processes that constrained bacterial assembly were closely associated with eutrophication levels. Moreover, increasing eutrophication significantly (P < 0.001) attenuated the distance decay rate, with a random spatial distribution of BCCs in the undisturbed location. In contrast, the complexity of interspecies interaction was enhanced under moderate eutrophication levels but declined under heavy eutrophication. Changes in the relative abundances of 27 bacterial families were significantly correlated with eutrophication levels. Notably, the pattern of enrichment or decrease for a given bacterial family was consistent with its known ecological functions. Our findings demonstrate that the magnitude of changes in BCCs and underlying determinism are dependent on eutrophication levels. However, the buffer capacity of bacterioplankton community is limited, with disrupted interspecies interaction occurring under heavy eutrophication. As such, bacterial assemblages are sensitive to changes in environmental conditions and could thus potentially serve as bio-indicators for increasing eutrophication. © 2017 Elsevier Ltd


Ding H.,Ningbo University | Ding H.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Jiao H.-F.,Ningbo Academy of Oceanology and Fishery | Shi X.-Z.,Ningbo University | And 5 more authors.
Sensors and Actuators, B: Chemical | Year: 2017

Two types of molecularly imprinted silica layers appended to quantum dots (MIP-QDs) for sulfonamides (SAs) were fabricated using reverse microemulsion surface grafting with 3-aminopropyltriethoxysilane as a functional monomer and sulfadimethoxine (SDM) and sulfadiazine (SDZ) as template molecules. The characterizations of both MIP-QDs were analyzed by ATR-FT-IR, SEM, and TEM, and their imprinting and specific fluorescence quenching mechanisms were elucidated through selectivity fluorescence response experiments. Finally, the MIP-QDs synthesized with SDZ as template exhibited excellent selective fluorescence quenching properties, which were attributed to the complementary imprinting cavities of the MIP-QDs to the templates and to the specific interactions between the functional monomer and the template molecules. Furthermore, a selective MIP-QD-based fluorescence optosensing sensor for the highly selective and sensitive recognition of SDZ was developed and successfully applied to detect SDZ in seawater and shrimp samples. Under optimal conditions, linear relationships with correlation coefficients 0.9971 were obtained along the range of 0.005–1.5 mg/L to SDZ. The limit of detection was 0.004 μg/L and 0.79 μg/kg for SDZ in seawater and shrimp samples, respectively. Excellent recoveries (82.7–99.9%) with a relative standard deviation (below 6.1%) were obtained for both samples spiked with three levels of SDZ. The proposed MIP-QD method provides a powerful tool for the rapid and sensitive determination of SDZ in real samples. © 2017 Elsevier B.V.


Jian Q.,Post University | Zhu X.,Post University | Chen J.,Post University | Zhu Z.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | And 4 more authors.
Journal of Applied Phycology | Year: 2017

1-Octen-3-ol is a major volatile metabolite produced by red seaweed Pyropia haitanensis under stresses. Using a metabolic profiling approach, we identified metabolites in P. haitanensis affected by 1-octen-3-ol treatment. The thalli were exposed to 1-octen-3-ol for 0.5 and 1 h. Using a non-targeted GC-MS analysis, 246 peaks in control and 1-octen-3-ol-treated P. haitanensis were detected. Among them, 72 metabolites were identified. Further statistical analysis revealed that these 72 metabolites covered different types of primary metabolism and secondary metabolism pathways, including organic acids, carbohydrates, amino acids, glycols, and fatty acids. PCA and PLS-DA analyses revealed that the metabolic composition differed between the control and 1-octen-3-ol-treated samples, and the main metabolites contributing to the dispersion were fatty acid, citric acid, some sugars, and amino acids. However, the metabolic compositions between two 1-octen-3-ol-treated samples (0.5 and 1 h) were similar. Pyropia haitanensis treated with 1-octen-3-ol showed reduced levels of certain free fatty acids and an increased level of monoacylglycerol. In addition, the synthesis of many amino acids and photosynthetic product galactosylglycerol was increased, along with some essential metabolites in the primary metabolic pathways, such as glycerol-3-phosphate, organic acids, and others. These results indicated that P. haitanensis recognized 1-octen-3-ol, which accelerated its primary metabolism to promote cell growth under stresses. These findings provided useful information on how algae respond to stress-induced signal. © 2017 Springer Science+Business Media Dordrecht


Xiong J.,Ningbo University | Xiong J.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Zhu J.,Ningbo University | Dai W.,Ningbo University | And 5 more authors.
Environmental Microbiology | Year: 2017

Increasing evidence has emerged a tight link among the gut microbiota, host age and health status. This osculating interplay impedes the definition of gut microbiome features associated with host health from that in developmental stages. Consequently, gut microbiota-based prediction of health status is promising yet not well established. Here we firstly tracked shrimp gut microbiota (N = 118) over an entire cycle of culture; shrimp either stayed healthy or progressively transitioned into severe disease. The results showed that the gut microbiota were significantly distinct over shrimp developmental stages and disease progression. Null model and phylogenetic-based mean nearest taxon distance (MNTD) analyses indicated that deterministic processes that governed gut community became less important as the shrimp aged and disease progressed. The predicted gut microbiota age (using the profiles of age-discriminatory bacterial species as independent variables) fitted well (r = 0.996; P < 0.001) with the age of healthy subjects, while this defined trend was disrupted by disease. Microbiota-for-age Z-scores (MAZ, here defined as immaturity) were relative stable among healthy shrimp, but sharply decreased when disease emerged. By distinguishing between age- and disease- discriminatory taxa, we developed a model, bacterial indicators of shrimp health status, to diagnose disease from healthy subjects with 91.5% accuracy. Notably, the relative abundances of the bacterial indicators were indicative for shrimp disease severity. These findings, in aggregate, add our understanding on the gut community assembly patterns over shrimp developmental stages and disease progression. In addition, shrimp disease initiation and severity can be accurately diagnosed using gut microbiota immaturity and bacterial indicators. © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd


Li X.,Ningbo University | Jiao H.-F.,Ningbo Academy of Oceanology and Fishery | Shi X.-Z.,Ningbo University | Shi X.-Z.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | And 5 more authors.
Biosensors and Bioelectronics | Year: 2018

A novel molecularly imprinted silica layer appended to FeSe quantum dots (MIP-FeSe-QDs) was fabricated and utilized as a recognition element to develop a selective and sensitive fluorescent nanosensor for cyfluthrin (CYF) determination. The MIP-FeSe-QDs were characterized by fluorescence spectrometry, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. Excellent selectivity and high sensitivity of MIP-FeSe-QDs to CYF molecules were observed based on the fluorescence quenching of FeSe-QDs. Under optimal conditions, a good linear relationship was found between fluorescence quenching effect and increased CYF concentration within 0.010–0.20 mg/L, with a correlation coefficient of 0.9911. The practicality of the developed sensor method for CYF detection in fish and sediment samples was further validated. Good recoveries ranging from 88.0% to 113.9% with<6.8% relative standard deviations were obtained. The detection limits of CYF in sediment and fish samples were 1.3 and 1.0 µg/kg, respectively. This study established a novel, rapid fluorescent nanosensor detection method based on MIP-QDs for successfully analyzing CYF in fish and sediment samples. © 2017 Elsevier B.V.


Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Yu W.,Ningbo University | Yu W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | And 5 more authors.
Applied Microbiology and Biotechnology | Year: 2017

Increasing evidence has revealed a close interplay between the gut bacterial communities and host growth performance. However, until recently, studies generally ignored the contribution of eukaryotes, endobiotic organisms. To fill this gap, we used Illumina sequencing technology on eukaryotic 18S rRNA gene to compare the structures of gut eukaryotic communities among cohabitating retarded, overgrown, and normal shrimp obtained from identically managed ponds. Results showed that a significant difference between gut eukaryotic communities differed significantly between water and intestine and among three shrimp categories. Structural equation modeling revealed that changes in the gut eukaryotic community were positively related to digestive enzyme activities, which in turn influenced shrimp growth performance (λ = 0.97, P < 0.001). Overgrown shrimp exhibited a more complex and cooperative gut eukaryotic interspecies interaction than retarded and normal shrimp, which may facilitate their nutrient acquisition efficiency. Notably, the distribution of dominant eukaryotic genera and shifts in keystone species were closely concordant with shrimp growth performance. In summary, this study provides an integrated overview on direct roles of gut eukaryotic communities in shrimp growth performance instead of well-studied bacterial assembly. © 2017 Springer-Verlag GmbH Germany


Wang K.,Ningbo University | Wang K.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Chen H.,Ningbo University | Zhao Q.,Ningbo University | And 8 more authors.
Environmental microbiology | Year: 2015

The underlying mechanisms of microbial community assembly in connective coastal environments are unclear. The coastal water area of northern Zhejiang, East China Sea, is a complex marine ecosystem with multiple environmental gradients, where the distributions and determinants of bacterioplankton communities remain unclear. We collected surface water samples from 95 sites across eight zones in this area for investigating bacterial community with 16S rRNA gene high-throughput sequencing. Bacterial alpha-diversity exhibits strong associations with water chemical parameters and latitude, with 75.5% of variation explained by suspended particle. The composition of dominant phyla can group the sampling sites into four bacterial provinces, and most key discriminant phyla and families/genera of each province strongly associate with specific environmental features, suggesting that local environmental conditions shape the biogeographic provincialism of bacterial taxa. At a broader and finer phylogenetic scale, bacterial beta-diversity is dominantly explained by the shared variation of environmental and spatial factors (63.3%); meanwhile, the environmental determinants of bacterial β-diversity generally exhibit spatially structured patterns, suggesting that bacterial assembly in surface water is highly controlled by spatially structured environmental gradients in this area. This study provides evidence for the unique biogeographic pattern of bacterioplankton communities at an entire scale of this marine ecosystem. © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.


PubMed | Lihuili Hospital of Ningbo Medical Center, Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture and Zhejiang University
Type: | Journal: International immunopharmacology | Year: 2016

As a sulfated polysaccharide, carrageenan has been widely used as common food additive.In the present study, we investigated the effects of -carrageenan on TNBS-induced gut inflammation in mice. BALB/c mice were pretreated with -carrageenan for 14days prior to the administration of TNBS.Our results showed that -carrageenan pretreatment aggravated the loss of body weight and further increased the mortality rate. Histological and morphological analyses revealed that the TNBS-induced colonic inflammation was deteriorated by the -carrageenan administration. The ratio of CD4(+)CD25(+)CD127dim/CD4(+) of the -carrageenan+TNBS groups was significantly lower than that of the TNBS group. The expression of IL-2, TNF- and IL-6 was significantly increased, whereas the expression of IL-10 was significantly decreased in the -carrageenan+TNBS groups. In addition, -carrageenan, together with TNBS, decreased the enzyme activity of SOD and GSH-px and up-regulated the expression of TLR4, NF-B, p-ERK, p-JNK, p-Jun., IL-8 and MDA in the colonic mucosa.-Carrageenan aggravated the TNBS-induced intestinal inflammation, and such an effect could be associated with the oxidative stress and activation of TLR4-NF-B and MAPK/ERK1/2 pathway.


Xiong J.,Ningbo University | Xiong J.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2016

High-density aquaculture has led to increasing occurrences of diseases in shrimp. Thus, it is imperative to establish effective and quantitative strategies for preventing and predicting these diseases. Water quality indices and investigations of specific pathogen abundance provide only a qualitative evaluation of the risk of shrimp disease and can be inaccurate. To address these shortcomings, we introduced intestinal indicative assemblages as independent variables with which to quantitatively predict incidences of shrimp disease. Given the ignorance regarding the niches differences in the shrimp intestine throughout its developmental stages, the use of probiotics in aquaculture has had limited success. Therefore, we propose the exploration of effective probiotic bacteria from shrimp intestinal flora and the establishment of therapeutic strategies dependent on shrimp age. Following ecological selection principles, we hypothesize that the larval stage provides the best opportunity to establish a desired gut microbiota through preemptive colonization of the treated rearing water with known probiotics. To employ this strategy, however, substantial barriers must be overcome. © 2016 Springer-Verlag Berlin Heidelberg


PubMed | Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture, Yangzhou University, Shanghai Ocean University, Hellenic Center for Marine Research and 3 more.
Type: | Journal: Scientific reports | Year: 2016

Silver nanoparticles (AgNPs) demonstrating good antimicrobial activity are widely used in many fields. However, the impact of AgNPs on the community structures of marine biofilms that drive biogeochemical cycling processes and the recruitment of marine invertebrate larvae remains unknown. Here, we employed MiSeq sequencing technology to evaluate the bacterial communities of 28-day-old marine biofilms formed on glass, polydimethylsiloxane (PDMS), and PDMS filled with AgNPs and subsequently tested the influence of these marine biofilms on plantigrade settlement by the mussel Mytilus coruscus. AgNP-filled PDMS significantly reduced the dry weight and bacterial density of biofilms compared with the glass and PDMS controls. AgNP incorporation impacted bacterial communities by reducing the relative abundance of Flavobacteriaceae (phylum: Bacteroidetes) and increasing the relative abundance of Vibrionaceae (phylum: Proteobacteria) in 28-day-old biofilms compared to PDMS. The settlement rate of M. coruscus on 28-day-old biofilms developed on AgNPs was lower by >30% compared to settlement on control biofilms. Thus, the incorporation of AgNPs influences biofilm bacterial communities in the marine environment and subsequently inhibits mussel settlement.

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