Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture

Ningbo, China

Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture

Ningbo, China

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Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Zhang J.,Ningbo University | Tu Q.,Zhejiang University | And 4 more authors.
Chemosphere | Year: 2017

Anthropogenic perturbations impose negative effects on coastal ecosystems, such as increasing levels of eutrophication. Given the biogeochemical significance of microorganisms, understanding the processes and mechanisms underlying their spatial distribution under changing environmental conditions is critical. To address this question, we examined how coastal bacterioplankton communities respond to increasing eutrophication levels created by anthropogenic perturbations. The results showed that the magnitude of changes in the bacterioplankton community compositions (BCCs) and the importance of deterministic processes that constrained bacterial assembly were closely associated with eutrophication levels. Moreover, increasing eutrophication significantly (P < 0.001) attenuated the distance decay rate, with a random spatial distribution of BCCs in the undisturbed location. In contrast, the complexity of interspecies interaction was enhanced under moderate eutrophication levels but declined under heavy eutrophication. Changes in the relative abundances of 27 bacterial families were significantly correlated with eutrophication levels. Notably, the pattern of enrichment or decrease for a given bacterial family was consistent with its known ecological functions. Our findings demonstrate that the magnitude of changes in BCCs and underlying determinism are dependent on eutrophication levels. However, the buffer capacity of bacterioplankton community is limited, with disrupted interspecies interaction occurring under heavy eutrophication. As such, bacterial assemblages are sensitive to changes in environmental conditions and could thus potentially serve as bio-indicators for increasing eutrophication. © 2017 Elsevier Ltd


Ding H.,Ningbo University | Ding H.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Jiao H.-F.,Ningbo Academy of Oceanology and Fishery | Shi X.-Z.,Ningbo University | And 5 more authors.
Sensors and Actuators, B: Chemical | Year: 2017

Two types of molecularly imprinted silica layers appended to quantum dots (MIP-QDs) for sulfonamides (SAs) were fabricated using reverse microemulsion surface grafting with 3-aminopropyltriethoxysilane as a functional monomer and sulfadimethoxine (SDM) and sulfadiazine (SDZ) as template molecules. The characterizations of both MIP-QDs were analyzed by ATR-FT-IR, SEM, and TEM, and their imprinting and specific fluorescence quenching mechanisms were elucidated through selectivity fluorescence response experiments. Finally, the MIP-QDs synthesized with SDZ as template exhibited excellent selective fluorescence quenching properties, which were attributed to the complementary imprinting cavities of the MIP-QDs to the templates and to the specific interactions between the functional monomer and the template molecules. Furthermore, a selective MIP-QD-based fluorescence optosensing sensor for the highly selective and sensitive recognition of SDZ was developed and successfully applied to detect SDZ in seawater and shrimp samples. Under optimal conditions, linear relationships with correlation coefficients 0.9971 were obtained along the range of 0.005–1.5 mg/L to SDZ. The limit of detection was 0.004 μg/L and 0.79 μg/kg for SDZ in seawater and shrimp samples, respectively. Excellent recoveries (82.7–99.9%) with a relative standard deviation (below 6.1%) were obtained for both samples spiked with three levels of SDZ. The proposed MIP-QD method provides a powerful tool for the rapid and sensitive determination of SDZ in real samples. © 2017 Elsevier B.V.


Wu W.,Zhejiang University | Zhen Z.,Zhejiang University | Niu T.,Zhejiang University | Zhu X.,Zhejiang University | And 5 more authors.
Mediators of Inflammation | Year: 2017

Background. The dietary usage of carrageenan as common food additive has increased observably over the last 50 years. But there is substantial controversy about its safety. Methods. We investigated whether the k-carrageenan could enhance lipopolysaccharide-induced IL-8 expression by studying its actions on the TLR4-NF-kB pathway. The aggravating effect of k-carrageenan on Citrobacter freundii DBS100-induced intestinal inflammation was also investigated in a mouse model. Results. Our data show that k-carrageenan pretreatment promoted LPS-induced IL-8 expression in HT-29 cells. Although CD14, MD-2, and TLR4 were upregulated, the binding of LPS was not enhanced. However, the pathway of Bcl10-NF-B was triggered. Interestingly, k-carrageenan competitively blocked the binding of FITC-LPS. Furthermore, pretreatment with k-carrageenan for one week previous to gavage with C. freundii DBS100 markedly aggravated weight loss, mortality, and colonic damage. The secretion of cytokines was unbalanced and the ratio of Tregs was decreased significantly. In addition, k-carrageenan, together with C. freundii DBS100, enhanced the transcription and secretion of TLR4 and NF-kB. Conclusions. K-Carrageenan can synergistically activate LPS-induced inflammatory through the Bcl10-NF-kB pathway, as indicated by its aggravation of C. freundii DBS100-induced colitis in mice. General Significance. Our results suggest that k-carrageenan serves as a potential inflammatory agent that magnifies existing intestinal inflammation. © 2017 Wei Wu et al.


Wang K.,Ningbo University | Wang K.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Chen H.,Ningbo University | Zhao Q.,Ningbo University | And 8 more authors.
Environmental microbiology | Year: 2015

The underlying mechanisms of microbial community assembly in connective coastal environments are unclear. The coastal water area of northern Zhejiang, East China Sea, is a complex marine ecosystem with multiple environmental gradients, where the distributions and determinants of bacterioplankton communities remain unclear. We collected surface water samples from 95 sites across eight zones in this area for investigating bacterial community with 16S rRNA gene high-throughput sequencing. Bacterial alpha-diversity exhibits strong associations with water chemical parameters and latitude, with 75.5% of variation explained by suspended particle. The composition of dominant phyla can group the sampling sites into four bacterial provinces, and most key discriminant phyla and families/genera of each province strongly associate with specific environmental features, suggesting that local environmental conditions shape the biogeographic provincialism of bacterial taxa. At a broader and finer phylogenetic scale, bacterial beta-diversity is dominantly explained by the shared variation of environmental and spatial factors (63.3%); meanwhile, the environmental determinants of bacterial β-diversity generally exhibit spatially structured patterns, suggesting that bacterial assembly in surface water is highly controlled by spatially structured environmental gradients in this area. This study provides evidence for the unique biogeographic pattern of bacterioplankton communities at an entire scale of this marine ecosystem. © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.


PubMed | Lihuili Hospital of Ningbo Medical Center, Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture and Zhejiang University
Type: | Journal: International immunopharmacology | Year: 2016

As a sulfated polysaccharide, carrageenan has been widely used as common food additive.In the present study, we investigated the effects of -carrageenan on TNBS-induced gut inflammation in mice. BALB/c mice were pretreated with -carrageenan for 14days prior to the administration of TNBS.Our results showed that -carrageenan pretreatment aggravated the loss of body weight and further increased the mortality rate. Histological and morphological analyses revealed that the TNBS-induced colonic inflammation was deteriorated by the -carrageenan administration. The ratio of CD4(+)CD25(+)CD127dim/CD4(+) of the -carrageenan+TNBS groups was significantly lower than that of the TNBS group. The expression of IL-2, TNF- and IL-6 was significantly increased, whereas the expression of IL-10 was significantly decreased in the -carrageenan+TNBS groups. In addition, -carrageenan, together with TNBS, decreased the enzyme activity of SOD and GSH-px and up-regulated the expression of TLR4, NF-B, p-ERK, p-JNK, p-Jun., IL-8 and MDA in the colonic mucosa.-Carrageenan aggravated the TNBS-induced intestinal inflammation, and such an effect could be associated with the oxidative stress and activation of TLR4-NF-B and MAPK/ERK1/2 pathway.


Zhu J.,Ningbo University | Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Qiu Q.,Ningbo University | And 4 more authors.
Microbial Ecology | Year: 2016

Intestinal bacterial communities play a pivotal role in promoting host health; therefore, the disruption of intestinal bacterial homeostasis could result in disease. However, the effect of the occurrences of disease on intestinal bacterial community assembly remains unclear. To address this gap, we compared the multifaceted ecological differences in maintaining intestinal bacterial community assembly between healthy and diseased shrimps. The neutral model analysis shows that the relative importance of neutral processes decreases when disease occurs. This pattern is further corroborated by the ecosphere null model, revealing that the bacterial community assembly of diseased samples is dominated by stochastic processes. In addition, the occurrence of shrimp disease reduces the complexity and cooperative activities of species-to-species interactions. The keystone taxa affiliated with Alphaproteobacteria and Actinobacteria in healthy shrimp gut shift to Gammaproteobacteria species in diseased shrimp. Changes in intestinal bacterial communities significantly alter biological functions in shrimp. Within a given metabolic pathway, the pattern of enrichment or decrease between healthy and deceased shrimp is correlated with its functional effects. We propose that stressed shrimp are more prone to invasion by alien strains (evidenced by more stochastic assembly and higher migration rate in diseased shrimp), which, in turn, disrupts the cooperative activity among resident species. These findings greatly aid our understanding of the underlying mechanisms that govern shrimp intestinal community assembly between health statuses. © 2016 Springer Science+Business Media New York


Xiong J.,Ningbo University | Xiong J.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2016

High-density aquaculture has led to increasing occurrences of diseases in shrimp. Thus, it is imperative to establish effective and quantitative strategies for preventing and predicting these diseases. Water quality indices and investigations of specific pathogen abundance provide only a qualitative evaluation of the risk of shrimp disease and can be inaccurate. To address these shortcomings, we introduced intestinal indicative assemblages as independent variables with which to quantitatively predict incidences of shrimp disease. Given the ignorance regarding the niches differences in the shrimp intestine throughout its developmental stages, the use of probiotics in aquaculture has had limited success. Therefore, we propose the exploration of effective probiotic bacteria from shrimp intestinal flora and the establishment of therapeutic strategies dependent on shrimp age. Following ecological selection principles, we hypothesize that the larval stage provides the best opportunity to establish a desired gut microbiota through preemptive colonization of the treated rearing water with known probiotics. To employ this strategy, however, substantial barriers must be overcome. © 2016 Springer-Verlag Berlin Heidelberg


Dai W.-F.,Ningbo University | Yang S.-Y.,Ningbo University | Que Z.-J.,Ningbo University | Xiong J.-B.,Ningbo University | Xiong J.-B.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture
Huanjing Kexue/Environmental Science | Year: 2016

Plankton microeukaryotes are primary producers, bacterial grazers and parasites in the ocean, thus contributing essential roles in marine ecosystem stability. For this reason, understanding how the microeukaryotic community responds to increasing temperature created by thermal discharges is key to evaluating the ecological and environmental consequences of a power plant. In this study, using an Illumina sequencing based analysis of eukaryotic 18S rDNA gene, we investigated the compositions of microeukaryotic community along a thermal gradient caused by the discharge from the Wusha Mountain power plant in Xiangshan Bay. The plankton microeukaryotic communities were dominated by Protalveolata, Ciliophora, Dinoflagellata and Cercozoa. A multivariate regression tree revealed that mircoeukaryotic diversity was primarily controlled by dissolved oxygen (DO), followed by nitrate and temperature. Thermal discharge significantly altered the compositions of microeukaryotic community, evidenced by an analysis of similarity (Global RANOSIM=0.422, P<0.001). A forward selection procedure showed that the variations of microeukaryotic community were primarily shaped by geographic distance, DO, chlorophyll a, and temperature. The spatial distribution of microeukaryotic community followed a distance-decay for similarity relationship, with a turnover of 0.002. In addition, 15 sensitive eukaryotic families were screened, the relative abundances of which were significantly associated with the discharge-induced temperature gradient. For a given eukaryotic family, the pattern of enrichment or decline was consistent with its known ecological function, which could be served as bio-indicators for temperature anomalies. Collectively, this study demonstrates the spatial pattern of microeukaryotic community in responses to increasing temperature, and provides sensitive bio-indicators for evaluating the ecological consequences of thermal discharge. © 2016, Science Press. All right reserved.


PubMed | Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture, Yangzhou University, Shanghai Ocean University, Hellenic Center for Marine Research and 3 more.
Type: | Journal: Scientific reports | Year: 2016

Silver nanoparticles (AgNPs) demonstrating good antimicrobial activity are widely used in many fields. However, the impact of AgNPs on the community structures of marine biofilms that drive biogeochemical cycling processes and the recruitment of marine invertebrate larvae remains unknown. Here, we employed MiSeq sequencing technology to evaluate the bacterial communities of 28-day-old marine biofilms formed on glass, polydimethylsiloxane (PDMS), and PDMS filled with AgNPs and subsequently tested the influence of these marine biofilms on plantigrade settlement by the mussel Mytilus coruscus. AgNP-filled PDMS significantly reduced the dry weight and bacterial density of biofilms compared with the glass and PDMS controls. AgNP incorporation impacted bacterial communities by reducing the relative abundance of Flavobacteriaceae (phylum: Bacteroidetes) and increasing the relative abundance of Vibrionaceae (phylum: Proteobacteria) in 28-day-old biofilms compared to PDMS. The settlement rate of M. coruscus on 28-day-old biofilms developed on AgNPs was lower by >30% compared to settlement on control biofilms. Thus, the incorporation of AgNPs influences biofilm bacterial communities in the marine environment and subsequently inhibits mussel settlement.


Li Y.-F.,Shanghai Ocean University | Guo X.-P.,Shanghai Ocean University | Chen Y.-R.,Shanghai Ocean University | Ding D.-W.,State Oceanic Administration | And 3 more authors.
Journal of the Marine Biological Association of the United Kingdom | Year: 2016

Mussels are typical macrofouling organisms in the world. In this study, the interaction between the settlement of Mytilus coruscus plantigrades and bacterial community on coloured substrata was determined. Bacterial communities in biofilms developed on seven coloured substrata were analysed by Illumina Miseq sequencing. The mussel settlement response to coloured substrata with no biofilms was also examined. Flavobacteria, Alphaproteobacteria and Gammaproteobacteria were the first, second and third most dominant groups in seven biofilm samples. The results suggest that the inducing activities of these biofilms on plantigrade settlement varied with coloured substrata and the lowest percentage of settlement was observed on biofilms on the green substratum. High-throughput sequencing showed that bacterial community in biofilms also changed with the substratum colour. No significant difference in the inducing activity on plantigrade settlement was observed between the coloured substrata with no biofilms. Thus, difference in plantigrade settlement response may be correlated to the changes in bacterial community on coloured substrata. This finding extends current knowledge of interaction among mussel settlement and bacterial community variability. Copyright © Marine Biological Association of the United Kingdom 2016

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