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Wang K.,Ningbo University | Wang K.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Chen H.,Ningbo University | Zhao Q.,Ningbo University | And 8 more authors.
Environmental microbiology | Year: 2015

The underlying mechanisms of microbial community assembly in connective coastal environments are unclear. The coastal water area of northern Zhejiang, East China Sea, is a complex marine ecosystem with multiple environmental gradients, where the distributions and determinants of bacterioplankton communities remain unclear. We collected surface water samples from 95 sites across eight zones in this area for investigating bacterial community with 16S rRNA gene high-throughput sequencing. Bacterial alpha-diversity exhibits strong associations with water chemical parameters and latitude, with 75.5% of variation explained by suspended particle. The composition of dominant phyla can group the sampling sites into four bacterial provinces, and most key discriminant phyla and families/genera of each province strongly associate with specific environmental features, suggesting that local environmental conditions shape the biogeographic provincialism of bacterial taxa. At a broader and finer phylogenetic scale, bacterial beta-diversity is dominantly explained by the shared variation of environmental and spatial factors (63.3%); meanwhile, the environmental determinants of bacterial β-diversity generally exhibit spatially structured patterns, suggesting that bacterial assembly in surface water is highly controlled by spatially structured environmental gradients in this area. This study provides evidence for the unique biogeographic pattern of bacterioplankton communities at an entire scale of this marine ecosystem. © 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.

PubMed | Lihuili Hospital of Ningbo Medical Center, Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture and Zhejiang University
Type: | Journal: International immunopharmacology | Year: 2016

As a sulfated polysaccharide, carrageenan has been widely used as common food additive.In the present study, we investigated the effects of -carrageenan on TNBS-induced gut inflammation in mice. BALB/c mice were pretreated with -carrageenan for 14days prior to the administration of TNBS.Our results showed that -carrageenan pretreatment aggravated the loss of body weight and further increased the mortality rate. Histological and morphological analyses revealed that the TNBS-induced colonic inflammation was deteriorated by the -carrageenan administration. The ratio of CD4(+)CD25(+)CD127dim/CD4(+) of the -carrageenan+TNBS groups was significantly lower than that of the TNBS group. The expression of IL-2, TNF- and IL-6 was significantly increased, whereas the expression of IL-10 was significantly decreased in the -carrageenan+TNBS groups. In addition, -carrageenan, together with TNBS, decreased the enzyme activity of SOD and GSH-px and up-regulated the expression of TLR4, NF-B, p-ERK, p-JNK, p-Jun., IL-8 and MDA in the colonic mucosa.-Carrageenan aggravated the TNBS-induced intestinal inflammation, and such an effect could be associated with the oxidative stress and activation of TLR4-NF-B and MAPK/ERK1/2 pathway.

Chen H.,Post University | Chen H.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Jian Q.,Post University | Luo Q.,Post University | And 3 more authors.
Journal of Applied Phycology | Year: 2015

Oligosaccharides can elicit a defense-related response in marine algae or seaweeds. This study aimed to investigate the possible application of oligosaccharides as biological elicitors for algal farming by measuring their antirotting effects on Pyropia haitanensis. The concentration of oligoagars and the time of treatment were optimized by examining their effects on the percentage of rotting in thalli. Oxidative burst and expression of defense-related genes were examined. The optimized conditions obtained were then applied in an aquaculture setting for P. haitanensis. Elicitation with oligoagars at 100 μg mL−1 for 2 h produced the optimal rot resistance effect, and oligoagars delayed the severe rotting of thalli by more than 13 days. Oligoagars elicited rapid respiration in thalli. The expression levels of Phrboh and Phhsp70 genes were up-regulated whereas Phsod was down-regulated. During aquaculture, treatment with oligoagars when thalli were 30 days old and when conchospores adhered to nets improved growth, increased yield of products, and delayed the maturation of thalli. However, treatment when conchospores adhered to nets had better results and was more convenient and practical. Overall, we found that oligoagars have potential applications as elicitors for biological farming and can be applied in the aquaculture of P. haitanensis with promising results. © 2015 Springer Science+Business Media Dordrecht

Zhu J.,Ningbo University | Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Qiu Q.,Ningbo University | And 4 more authors.
Microbial Ecology | Year: 2016

Intestinal bacterial communities play a pivotal role in promoting host health; therefore, the disruption of intestinal bacterial homeostasis could result in disease. However, the effect of the occurrences of disease on intestinal bacterial community assembly remains unclear. To address this gap, we compared the multifaceted ecological differences in maintaining intestinal bacterial community assembly between healthy and diseased shrimps. The neutral model analysis shows that the relative importance of neutral processes decreases when disease occurs. This pattern is further corroborated by the ecosphere null model, revealing that the bacterial community assembly of diseased samples is dominated by stochastic processes. In addition, the occurrence of shrimp disease reduces the complexity and cooperative activities of species-to-species interactions. The keystone taxa affiliated with Alphaproteobacteria and Actinobacteria in healthy shrimp gut shift to Gammaproteobacteria species in diseased shrimp. Changes in intestinal bacterial communities significantly alter biological functions in shrimp. Within a given metabolic pathway, the pattern of enrichment or decrease between healthy and deceased shrimp is correlated with its functional effects. We propose that stressed shrimp are more prone to invasion by alien strains (evidenced by more stochastic assembly and higher migration rate in diseased shrimp), which, in turn, disrupts the cooperative activity among resident species. These findings greatly aid our understanding of the underlying mechanisms that govern shrimp intestinal community assembly between health statuses. © 2016 Springer Science+Business Media New York

Xiong J.,Ningbo University | Xiong J.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | Dai W.,Ningbo University | Dai W.,Collaborative Innovation Center for Zhejiang Marine High Efficiency and Healthy Aquaculture | And 2 more authors.
Applied Microbiology and Biotechnology | Year: 2016

High-density aquaculture has led to increasing occurrences of diseases in shrimp. Thus, it is imperative to establish effective and quantitative strategies for preventing and predicting these diseases. Water quality indices and investigations of specific pathogen abundance provide only a qualitative evaluation of the risk of shrimp disease and can be inaccurate. To address these shortcomings, we introduced intestinal indicative assemblages as independent variables with which to quantitatively predict incidences of shrimp disease. Given the ignorance regarding the niches differences in the shrimp intestine throughout its developmental stages, the use of probiotics in aquaculture has had limited success. Therefore, we propose the exploration of effective probiotic bacteria from shrimp intestinal flora and the establishment of therapeutic strategies dependent on shrimp age. Following ecological selection principles, we hypothesize that the larval stage provides the best opportunity to establish a desired gut microbiota through preemptive colonization of the treated rearing water with known probiotics. To employ this strategy, however, substantial barriers must be overcome. © 2016 Springer-Verlag Berlin Heidelberg

Dai W.-F.,Ningbo University | Yang S.-Y.,Ningbo University | Que Z.-J.,Ningbo University | Xiong J.-B.,Ningbo University | Xiong J.-B.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture
Huanjing Kexue/Environmental Science | Year: 2016

Plankton microeukaryotes are primary producers, bacterial grazers and parasites in the ocean, thus contributing essential roles in marine ecosystem stability. For this reason, understanding how the microeukaryotic community responds to increasing temperature created by thermal discharges is key to evaluating the ecological and environmental consequences of a power plant. In this study, using an Illumina sequencing based analysis of eukaryotic 18S rDNA gene, we investigated the compositions of microeukaryotic community along a thermal gradient caused by the discharge from the Wusha Mountain power plant in Xiangshan Bay. The plankton microeukaryotic communities were dominated by Protalveolata, Ciliophora, Dinoflagellata and Cercozoa. A multivariate regression tree revealed that mircoeukaryotic diversity was primarily controlled by dissolved oxygen (DO), followed by nitrate and temperature. Thermal discharge significantly altered the compositions of microeukaryotic community, evidenced by an analysis of similarity (Global RANOSIM=0.422, P<0.001). A forward selection procedure showed that the variations of microeukaryotic community were primarily shaped by geographic distance, DO, chlorophyll a, and temperature. The spatial distribution of microeukaryotic community followed a distance-decay for similarity relationship, with a turnover of 0.002. In addition, 15 sensitive eukaryotic families were screened, the relative abundances of which were significantly associated with the discharge-induced temperature gradient. For a given eukaryotic family, the pattern of enrichment or decline was consistent with its known ecological function, which could be served as bio-indicators for temperature anomalies. Collectively, this study demonstrates the spatial pattern of microeukaryotic community in responses to increasing temperature, and provides sensitive bio-indicators for evaluating the ecological consequences of thermal discharge. © 2016, Science Press. All right reserved.

Chen X.,Ningbo University | Wang K.,Ningbo University | Wang K.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Guo A.,Ningbo University | And 5 more authors.
Journal of Experimental Marine Biology and Ecology | Year: 2016

Phosphorus (P), primarily as phosphate, is considered as a key trophic factor of eutrophication in coastal environments. Information on the response of bacterial community to excess phosphate loading in oligotrophic coastal water could provide insights into the micro-ecological effects on the expanding trend of nutrient-enrichment in coastal water. In this study, temporal dynamic of bacterioplankton community composition was investigated using 16S Illumina MiSeq sequencing technique in oligotrophic seawater microcosms manipulated with no perturbation (control) and a certain level of phosphate by two loading modes: 1) one-off loading at the beginning of incubation; and 2) periodic loadings every two days over a 16-day duration. The results showed that overall bacterial alpha-diversity temporally changed and achieved the lowest value in phosphate loading (P-loading) microcosms at day 8; phosphate-addition increased the Shannon index and Pielou's evenness compared with those of the control at day 16. Bacterioplankton community turnover across all the treatments showed a similar temporal pattern. P-loading significantly affected bacterioplankton community composition with negative correlations of Methylophilaceae and Flavobacteriaceae and positive correlation of Saprospiraceae. Periodic loading treatments demonstrated a greater effect on community variation than one-off loading treatments did. Moreover, excess P-loading accelerated the temporal succession of the bacterioplankton community composition. These results provide insights into the pattern of bacterioplankton community phylogenetically responding to excess P-loading in the manipulated oligotrophic coastal system. © 2016 Elsevier B.V.

PubMed | Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture, Yangzhou University, Shanghai Ocean University, Hellenic Center for Marine Research and 3 more.
Type: | Journal: Scientific reports | Year: 2016

Silver nanoparticles (AgNPs) demonstrating good antimicrobial activity are widely used in many fields. However, the impact of AgNPs on the community structures of marine biofilms that drive biogeochemical cycling processes and the recruitment of marine invertebrate larvae remains unknown. Here, we employed MiSeq sequencing technology to evaluate the bacterial communities of 28-day-old marine biofilms formed on glass, polydimethylsiloxane (PDMS), and PDMS filled with AgNPs and subsequently tested the influence of these marine biofilms on plantigrade settlement by the mussel Mytilus coruscus. AgNP-filled PDMS significantly reduced the dry weight and bacterial density of biofilms compared with the glass and PDMS controls. AgNP incorporation impacted bacterial communities by reducing the relative abundance of Flavobacteriaceae (phylum: Bacteroidetes) and increasing the relative abundance of Vibrionaceae (phylum: Proteobacteria) in 28-day-old biofilms compared to PDMS. The settlement rate of M. coruscus on 28-day-old biofilms developed on AgNPs was lower by >30% compared to settlement on control biofilms. Thus, the incorporation of AgNPs influences biofilm bacterial communities in the marine environment and subsequently inhibits mussel settlement.

Yang J.-L.,State Oceanic Administration | Yang J.-L.,Shanghai Ocean University | Yang J.-L.,Collaborative Innovation Center for Zhejiang Marine High efficiency and Healthy Aquaculture | Li Y.-F.,Shanghai Ocean University | And 6 more authors.
Biofouling | Year: 2016

Abstract: This study investigated the effect of carbon nanotubes (CNTs) and titanium dioxide (TiO2) incorporated in PDMS on biofilm formation and plantigrade settlement of Mytilus coruscus. TiO2 increased bacterial density, and CNTs also increased bacterial density but reduced diatom density in biofilms after 28 days. Further analysis was conducted between bacterial communities on glass, PDMS, CNTs (0.5 wt%) and TiO2 (7.5 wt%). ANOSIM analysis revealed significant differences (R > 0.9) between seven, 14, 21 and 28 day-old bacterial communities. MiSeq sequencing showed that CNTs and TiO2 impacted the composition of 28 day-old bacterial communities by increasing the abundance of Proteobacteria and decreasing the abundance of Bacteroidetes. The maximum decreased settlement rate in 28 day-old biofilms on CNTs and TiO2 was > 50% in comparison to those on glass and PDMS. Thus, CNTs and TiO2 incorporated in PDMS altered the biomass and community composition of biofilms, and subsequently decreased mussel settlement. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Li Y.-F.,Shanghai Ocean University | Guo X.-P.,Shanghai Ocean University | Chen Y.-R.,Shanghai Ocean University | Ding D.-W.,State Oceanic Administration | And 3 more authors.
Journal of the Marine Biological Association of the United Kingdom | Year: 2016

Mussels are typical macrofouling organisms in the world. In this study, the interaction between the settlement of Mytilus coruscus plantigrades and bacterial community on coloured substrata was determined. Bacterial communities in biofilms developed on seven coloured substrata were analysed by Illumina Miseq sequencing. The mussel settlement response to coloured substrata with no biofilms was also examined. Flavobacteria, Alphaproteobacteria and Gammaproteobacteria were the first, second and third most dominant groups in seven biofilm samples. The results suggest that the inducing activities of these biofilms on plantigrade settlement varied with coloured substrata and the lowest percentage of settlement was observed on biofilms on the green substratum. High-throughput sequencing showed that bacterial community in biofilms also changed with the substratum colour. No significant difference in the inducing activity on plantigrade settlement was observed between the coloured substrata with no biofilms. Thus, difference in plantigrade settlement response may be correlated to the changes in bacterial community on coloured substrata. This finding extends current knowledge of interaction among mussel settlement and bacterial community variability. Copyright © Marine Biological Association of the United Kingdom 2016

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