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Shen F.,Nanjing University of Finance and Economics | Shen F.,Collaborative Innovation Center for Modern Grain Circulation and Safety | Wu Q.,Nanjing University of Finance and Economics | Su A.,Nanjing University of Finance and Economics | And 3 more authors.
Czech Journal of Food Sciences

The use of electronic nose and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) as rapid tools for detection of orange juice adulteration has been preliminarily investigated and compared. Freshly squeezed orange juices were tentatively adulterated with 100% concentrated orange juices at levels ranging from 0% to 30% (v/v). Then the E-nose response signals and FTIR spectra collected from samples were subjected to multivariate analysis by principal component analysis (PCA) and linear discriminant analysis (LDA). PCA indicated that authentic juices and adulterated ones could be approximately separated. For the classification of samples with different adulteration levels, the overall accuracy obtained by LDA in prediction was 91.7 and 87.5% for E-nose and ATR-FTIR, respectively. Gas chromatography-mass spectrometry (GC-MS) results verified that there existed an obvious holistic difference in flavour characteristics between fresh squeezed and concentrated juices. These results demonstrated that both E-nose and FTIR might be used as rapid screening techniques for the detection of this type of juice adulteration. Source

Mao B.,Collaborative Innovation Center for Modern Grain Circulation and Safety | He J.,Collaborative Innovation Center for Modern Grain Circulation and Safety | He J.,Victoria University of Melbourne | Cao J.,Collaborative Innovation Center for Modern Grain Circulation and Safety | And 2 more authors.
Procedia Computer Science

Food security is currently one of the most concerning problems in China. A traceability system is an effective method to improve the quality of food production. This system has been widely applied in several countries such as the US, Japan and the EU. However, in China, the creditability of traceability systems is not strong and many producers try to deceive the public by forging the data in such systems. In this paper, a video surveillance system-based traceability system is proposed which will significantly increase the forgery cost. In this system, subjects, such as vehicles or people, are firstly defined using a novel dynamic background model, then their trajectories are generated and connected using different cameras with a camera relation graph. The experimental results indicate that the proposed method can efficiently extract the object information from the video surveillance system and generate image-based traceability information to be used for further analysis. ©2015 Published by Elsevier B.V. Source

Yin X.,Jiangsu Academy of Agricultural Sciences | Yin X.,Key Laboratory of Food Quality and Safety of Jiangsu Province State Key Laboratory Breeding | Yin X.,Key Laboratory of Control Technology and Standard for Agro Product Quality and Safety | Zhu Z.,Jiangsu Academy of Agricultural Sciences | And 9 more authors.
Journal of Biotechnology

The strain A16, capable of degrading deoxynivalenol was isolated from a wheat field and identified preliminarily as Devosia sp. Here, we present the genome sequence of the Devosia sp. A16, which has a size of 5,032,994. bp, with 4913 coding sequences (CDSs). The annotated full genome sequence of the Devosia sp. A16 strain might shed light on the function of its degradation. © 2015 Elsevier B.V. Source

Qiu J.,Key Laboratory of Food Quality and Safety of Jiangsu Province | Qiu J.,Key Laboratory of Control Technology and Standard for Agro product Safety and Quality Nanjing | Qiu J.,Collaborative Innovation Center for Modern Grain Circulation and Safety | Qiu J.,Key Laboratory of Agro product Safety Risk Evaluation Nanjing | And 17 more authors.
European Journal of Plant Pathology

In 2012, we isolated mycotoxigenic fungi from maize kernels sourced in Jiangsu and Anhui provinces, China. Fusarium spp. accounted for most isolates detected, followed by Aspergillus and Penicillium spp. Of the Fusarium species, Fusarium verticillioides was the most prevalent, as revealed by direct PCR of DNA from kernel samples, followed by F. proliferatum and F. graminearum. Most F. verticillioides strains contained the FUM1 gene, the product of which plays a key role in fumonisin biosynthesis. In terms of mating type idiomorphs, about 80 % of all strains carried the MAT-1 allele, and 20 % carried MAT-2. The genetic structures of 192 representative F. verticillioides isolates were determined via an analysis of eight simple sequence repeat markers. This analysis revealed high-level gene diversity but low linkage disequilibrium in three populations grouped by the geographical areas in which they were collected. The three populations exhibited low-level genetic differentiation and high gene flow, indicating that geographical differences minimally influenced population differentiation; all three populations were potentially components of a larger, randomly mating population. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging. Source

Xu J.,Collaborative Innovation Center for Modern Grain Circulation and Safety | Xu J.,Jiangsu Academy of Agricultural Sciences | Wang H.,Collaborative Innovation Center for Modern Grain Circulation and Safety | Wang H.,Jiangsu Academy of Agricultural Sciences | And 10 more authors.
Food Control

Zearalenone (ZEN) is a mycotoxin produced mainly by various Fusarium species which occur naturally in many crops worldwide. ZEN causes reproductive disorders and hyperestrogenic syndromes in animals and humans. This study aimed to isolate ZEN-degrading bacteria to develop strategies for detoxifying ZEN contamination in cereal crops. We screened approximately 1000 colonies for degrading ability and found four strains were capable of degrading ZEN. We selected one strain ZDS-1 for further study because it showed the high ZEN-degrading ability. On the basis of morphological, physiological and phylogenetic analysis of its 16SrRNA, gyrA gene sequences, strain ZDS-1 was identified as Bacillus amyloliquefaciens. The optimal conditions for the biodegradation of ZEN by ZDS-1 were temperature; 30 °C, pH; 6.0-7.0, and cell concentration; 5 × 108 cfu/mL. ZDS-1 could degrade ZEN efficiently with the concentration from 1 mg/L to 100 mg/L. ZDS-1 not only could remove ZEN in the culture medium, but also could degrade ZEN in wheat. The ZEN removal by ZDS-1 was not due to binding or absorption, and during the process of ZEN degradation, no ZEN derivatives of ZEN were produced. These results suggested that Bacillus amyloliquefaciens ZDS-1 would be explored further for its ability to degrade ZEN in field trials. © 2016 Elsevier Ltd. Source

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