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Xu W.,Xiamen University | Xu W.,State Oceanic Administration | Xu W.,Collaborative Innovation Center for Development and Utilization of Marine Biological Resources | Pang K.-L.,National Taiwan Ocean University | And 2 more authors.
Microbial Ecology | Year: 2014

Knowledge about the presence and ecological significance of bacteria and archaea in the deep-sea environments has been well recognized, but the eukaryotic microorganisms, such as fungi, have rarely been reported. The present study investigated the composition and abundance of fungal community in the deep-sea sediments of the Pacific Ocean. In this study, a total of 1,947 internal transcribed spacer (ITS) regions of fungal rRNA gene clones were recovered from five sediment samples at the Pacific Ocean (water depths ranging from 5,017 to 6,986 m) using three different PCR primer sets. There were 16, 17, and 15 different operational taxonomic units (OTUs) identified from fungal-universal, Ascomycota-, and Basidiomycota-specific clone libraries, respectively. Majority of the recovered sequences belonged to diverse phylotypes of Ascomycota (25 phylotypes) and Basidiomycota (18 phylotypes). The multiple primer approach totally recovered 27 phylotypes which showed low similarities (≤97 %) with available fungal sequences in the GenBank, suggesting possible new fungal taxa occurring in the deep-sea environments or belonging to taxa not represented in the GenBank. Our results also recovered high fungal LSU rRNA gene copy numbers (3.52 × 106 to 5.23 × 107copies/g wet sediment) from the Pacific Ocean sediment samples, suggesting that the fungi might be involved in important ecological functions in the deep-sea environments. © 2014, Springer Science+Business Media New York.

Huang X.,Xiamen University | Ye H.,Xiamen University | Ye H.,Collaborative Innovation Center for Development and Utilization of Marine Biological Resources | Feng B.,Xiamen University | Huang H.,Xiamen University
Molecular and Cellular Endocrinology | Year: 2015

Insulin-like peptides (ILPs) have been proved to exist extensively in invertebrates and play critical roles in regulating growth, metabolism and reproduction. ILP signaling system has been well defined in insects, with all key components homologous with vertebrate IGF signaling; however, counterparts of IGF binding proteins (IGFBPs) in vertebrates are not included in this system because of lacking sufficient researches in the related aspect. The present study firstly reports the identification of three kinds of invertebrate IGF binding (IB) domain-containing protein genes from the mud crab Scylla paramamosain. Gene expression analysis suggested that they might be closely involved in ovarian development, but with separate roles. Subsequent bioinformatics analysis and in vitro experiments indicated that they are likely to serve as endogenous ILP-specific binding proteins in invertebrates. More importantly, based on the current evidence we inferred that in invertebrate, ILP system might take the place of IGF system in vertebrate species. © 2015 Elsevier Ireland Ltd.

Huang X.,Xiamen University | Ye H.,Xiamen University | Ye H.,Collaborative Innovation Center for Development and Utilization of Marine Biological Resources | Huang H.,Xiamen University | And 2 more authors.
General and Comparative Endocrinology | Year: 2015

Insulin-like growth factor (IGF) signaling system holds a central position in regulating growth and metabolism in vertebrates. As critical components of this system, the IGF-binding proteins (IGFBPs) play important roles in regulating the biological activities of IGFs. Recently, the single IGF-binding domain protein (SIBD) was identified in invertebrates and its sequence was highly homologous with the N-terminal domain of IGFBP. In view of the possible role as counterparts of vertebrate IGFBPs, SIBDs have attracted the ever-increasing attention. This study reports the identification of a 1284. bp SIBD gene (. Sp-SIBD) from a member of commercially important family of Portunidae. The tissue distribution analysis showed that Sp-SIBD was mainly expressed in the nervous tissues and hepatopancreas. RNA in situ hybridization analysis showed that the positive signals were predominantly distributed in the secretory cells of the hepatopancreas. Subsequently, we examined the effects of various stresses, including hyperosmotic stress, hyperthermia, activated stress and fasting, on glucose levels in the hemolymph and Sp-SIBD expressions in the hepatopancreas. Interestingly, we found that Sp-SIBD expression was strongly up-regulated in response to these catabolic circumstances. Given the previous findings of insulin-like peptides (ILPs) in invertebrates, we speculate that invertebrate ILPs and SIBDs promise to serve as a pair of counterparts of IGFs and IGFBPs from vertebrate species respectively. In this context, the combined results suggested, by analogy with IGFBP 1 from vertebrates, for the first time that SIBD might play a key physiological role by sequestering ILPs to inhibit energy-expensive growth until conditions are more favorable. © 2015 Elsevier Inc.

Li W.,Xiamen University | Huang H.,Xiamen University | Huang J.,Xiamen University | Ye H.,Xiamen University | Ye H.,Collaborative Innovation Center for Development and Utilization of Marine Biological Resources
Marine and Freshwater Behaviour and Physiology | Year: 2015

The horseshoe crab Tachypleus tridentatus is an ancient marine animal often referred to as a living fossil. Two full-length cDNA sequences were isolated from the ovary of T. tridentatus, and evidence was found that they are cyclin A (designated as TTCyA) and cyclin B (TTCyB). This is the first evidence of their presence in the Class Merostomata. The putative TTCyA cDNA was 3001 and TTCyB 1545 bp. The results of comparison and phylogenetic analysis suggest that the two sequences may have changed little in the horseshoe crab but have undergone rapid evolution in later arthropod groups. During the ovarian maturation cycle, the transcripts of TTCyA and TTCyB in the ovary increased significantly from the oogonia stage to the vitellogenic oocyte stage (p < 0.05). The results imply that both sequences are related to oogenesis in the horseshoe crab. © 2015, © 2015 Taylor & Francis.

Zeng H.,Xiamen University | Bao C.,Xiamen University | Huang H.,Xiamen University | Ye H.,Xiamen University | And 2 more authors.
Animal Reproduction Science | Year: 2016

In this study a full-length cDNA (Sp-RPCH) was cloned from the eyestalk ganglia of the mud crab Scylla paramamosain. Sp-RPCH is 660 base pairs in length and its open reading frame encodes a precursor that is predicted to be processed into a 25-residue signal peptide, a mature red pigment concentrating hormone (RPCH, an octapeptide), and a 75-residue precursor-related peptide. Phylogenetic analysis indicates that it clusters with other crustacean RPCHs and belongs to the adipokinetic hormone/RPCH peptide superfamily. Sp-RPCH gene expression was detected, using an end-point polymerase chain reaction (PCR), not only in the eyestalk ganglia but also in the brain and thoracic ganglia. Quantified using a real-time PCR, Sp-RPCH gene expression levels in the three tissues fluctuated along a cycle of ovarian maturation, with the levels progressively increased from stages I to IV, after which the expression levels decreased (although they remained significantly higher than stage I levels) when the ovary reached the mature stage (stage V). It was demonstrated using a patch clamp analysis that synthetic RPCH was able to evoke a Ca2+ current in dissociated brain neurons and synthetic RPCH significantly increased the mean oocyte diameter of the ovarian tissues co-cultured with the eyestalk ganglia, brain, or thoracic ganglia; the stimulatory effect of RPCH was absent when the nervous tissues were not included in the ovarian incubation. Animals administrated with RPCH had significantly higher levels of gonad-somatic index, hepatopancreas-somatic index, and vitellogenin gene expression, when compared to control animals receiving a saline injection. The combined results clearly show that RPCH is involved in ovarian maturation in the mud crab; the stimulatory effects of RPCH are likely mediated by its actions on the release from the nervous tissues of factor(s) that directly regulate vitellogenesis in the ovary and hepatopancreas. © 2015 Elsevier B.V.

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