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Zhao C.,Northeast Forestry University | Zhao C.,Collaborative Innovation Center for Development | Li Z.,Northeast Forestry University | Li C.,Northeast Forestry University | And 6 more authors.
International Journal of Biological Macromolecules | Year: 2015

The simultaneous ultrasonic/microwave-assisted extraction (UMAE) method is potentially useful for the extraction of polysaccharides from Taxus chinensis var. mairei fruits (TCFPs). In this study, we used a response surface methodology to identify optimal TCFPs extraction conditions. Optimal parameters were determined as follows: a liquid to raw material ratio of 33. mL/g, an extraction time of 10. min, a microwave power level of 560. W, and a fixed ultrasonic power of 50. W. Under the optimized conditions, TCFPs yields obtained by UMAE were 4.33. ±. 0.15%, a 1.79-fold increase compared with conventional heating reflux extraction (HRE). In addition, the extraction time used in UMAE was shorter than that required for HRE: 10 versus 90. min. UMAE is therefore a rapid and efficient method for the extraction of TCFPs. The inhibitory effect of TCFPs on S180 tumor growth in vivo was also studied. The tumor inhibition rate of TCFPs was 76.33%, indicating a tumor-inhibiting effect. Analysis of organ weights demonstrated that TCFPs exhibited no toxicity to liver, kidney, spleen, heart, or lung relative to a positive control group. TCFPs thus show antitumor activity with no organ toxicity. © 2015 Elsevier B.V.

Zhang G.,State Oceanic Administration | Zhang G.,Collaborative Innovation Center for Development | Yang Y.,State Oceanic Administration | Yang Y.,Collaborative Innovation Center for Development | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, designated strain F15T, was isolated from a deep-sea sediment of the western Pacific Ocean. The temperature, pH and NaCl ranges for growth were 4-50 ºC, pH 6-11 and 0-10 % (w/v), respectively. Strain F15T showed the highest 16S rRNA gene sequence similarity to Sagittula stellata E-37T (96.4 %), followed by Ponticoccus litoralis CL-GR66T (96.4 %), Antarctobacter heliothermus EL-219T (96.3 %) and Thalassococcus lentus YCS-24T (96.0 %). Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain F15T formed a lineage within the family Rhodobacteraceae of the class Alphaproteobacteria. The polar lipid profile of strain F15T comprised significant amounts of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified glycolipid and one unidentified phospholipid. The predominant cellular fatty acids were summed feature 8 (C18:1ω7c and/or C18:1ω6c, 40.2 %), anteiso-C15:0 (30.4 %) and anteiso-C17:0 (9.7 %). The genomic DNA G+C content of strain F15T was 60.2 mol% and the major respiratory quinone was Q-10. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain F15T is considered to represent a novel species of a new genus within the family Rhodobacteraceae, for which the name Alkalimicrobium pacificum gen. nov., sp. nov. is proposed. The type strain is F15T (=LMG 28107T=JCM 19851T=CGMCC 1.12763T=MCCC 1A09948T). © 2015 IUMS.

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