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Jiao J.,Northeast Forestry University | Gai Q.-Y.,Northeast Forestry University | Gai Q.-Y.,Heilongjiang University of Chinese Medicine | Wang W.,Collaborative Innovation Center for Development and Utilization of Forest Resources | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

In this work, Astragalus membranaceus hairy root cultures (AMHRCs) were exposed to ultraviolet radiation (UV-A, UV-B, and UV-C) for promoting isoflavonoid accumulation. The optimum enhancement for isoflavonoid production was achieved in 34-day-old AMHRCs elicited by 86.4 kJ/m2 of UV-B. The resulting isoflavonoid yield was 533.54 ± 13.61 μg/g dry weight (DW), which was 2.29-fold higher relative to control (232.93 ± 3.08 μg/g DW). UV-B up-regulated the transcriptional expressions of all investigated genes involved in isoflavonoid biosynthetic pathway. PAL and C4H were found to be two potential key genes that controlled isoflavonoid biosynthesis. Moreover, a significant increase was noted in antioxidant activity of extracts from UV-B-elicited AMHRCs (IC50 values = 0.85 and 1.08 mg/mL) in comparison with control (1.38 and 1.71 mg/mL). Overall, this study offered a feasible elicitation strategy to enhance isoflavonoid accumulation in AMHRCs and also provided a basis for metabolic engineering of isoflavonoid biosynthesis in the future. © 2015 American Chemical Society.


Wang F.,Collaborative Innovation Center for Development and Utilization of Forest Resources | Deng Q.,Collaborative Innovation Center for Development and Utilization of Forest Resources | Duan W.,Collaborative Innovation Center for Development and Utilization of Forest Resources | Sun Y.,Collaborative Innovation Center for Development and Utilization of Forest Resources | And 2 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2016

Blueberry wine was treated by individual or combined use of different clarifying agents such as chitosan, bentonite, gelatin and diatomite. The clarification effects were evaluated based on the analyses of relationship between amount of added clarifying agents and optical transmittance of blueberry wine. In order to optimize the chitosan-bentonite compound clarifying agent to clarify the optimal parameters, three operating parameters including optimal compound clarifying agent ratio, processing temperature and processing time were optimized based on clarification efficiency by response surface methodology. A quadratic polynomial regression model was developed indicating the effect of the three operating parameters on clarification efficiency. The results show that; the resulting compound clarifying agent to clarify the optimal parameters blueberry wine clarifying agent for the complex ratio of 2:1, the processing temperature of 50℃, treatment time 50 min. Under this condition, blueberry wine transmittance was 90.02%, and improved 37.80% compared without clarification. Under this clarification of the conditions for blueberry wine no significant effect on the physical and chemical composition and stability has improved greatly, the wine is clear and transparent, sweet and sour, rich flavor. © 2016, Editorial Office of Journal of CIFST. All right reserved.

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