Collaborative Innovation Center for Chinese Medicine in Qinba Mountains

Fengcheng, China

Collaborative Innovation Center for Chinese Medicine in Qinba Mountains

Fengcheng, China
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Yue Z.-G.,PLA Fourth Military Medical University | Yue Z.-G.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Wang X.,The First Hospital of Xian | Zhang X.-P.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2014

Ten compounds were isolated from the leaf of Eucommia ulmoides by means of recrystallization and chromatographic techniques such as D-101 macroporous resin, MCI resin, ODS gel, Sephadex LH-20 and Rp-HPLC. Their structures were identified by NMR spectral analyses as kaempferide 3-O-β-D-glucoside(1), quercetin-3-O-β-D-glucoside(2), quercetin(3), quercetin-3-O-β-D-xylosyl-(1→2)-β-D-galactoside(4), kaempferol-3-O-α-L-rhamnosyl-(1→6)-β-D-glucoside(5), (2S, 3S)-taxifolin 3-O-β-D-glucoside(6), 4-hydroxy cinnamic acid(7), (+)-cycloolivil(8), pinoresinol β-D-glucoside(9), squalene(10). Among them compounds 1, 5-7, 10 were isolated from the Eucommia genus for the first time. In the DPPH free radical scavenging assay, compound 2 exhibited significant activity(IC50 13.7 μmol·L-1), compared with vitamin C(IC50 59.9 μmol·L-1); compounds 1, 3 and 9 showed moderate activity(IC50 161, 137, 214 μmol·L-1), compared with 2, 6-di-tert-butyl-4-methylphenol(IC50 236 μmol·L-1); compound 4 and 6 showed weak activity(IC50 264, 299 μmol·L-1).


Wang W.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Cui J.-C.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Song X.-M.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Mei Q.-B.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Yue Z.-G.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains
Chinese Traditional and Herbal Drugs | Year: 2014

Objective: To analyze the genetic diversity of Panax japonicus var. major by inter simple sequence repeat (ISSR) molecular makers. Methods: Genetic diversity of 19 samples from main production areas was investigated by ISSR and analyzed by principal coordinate analysis and UPGMA cluster analysis. Results: A total of 181 bands were generated by 13 ISSR primers, among which 166 bands (91.71%) were polymorphic bands (PPB). The coefficient of genetic similarity ranged from 0.60 to 0.83. The results of UPGMA cluster analysis and principal coordinate analysis showed that the genetic diversity of P. japonicus var. major from the same region presented a geographical distribution regularity. Conclusion: The results of ISSR analysis reveals that P. japonicus var. major has a high genetic diversity level and the genetic relationship closely contacts with the geographical location. ©, 2014, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Zhang D.,PLA Fourth Military Medical University | Li H.,PLA Fourth Military Medical University | Li H.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Wang J.-B.,PLA Fourth Military Medical University
International Journal of Biological Macromolecules | Year: 2015

Amyloid protein depositions play crucial roles in a variety of degenerative disorders composing amyloidosis. There is a great interest in developing small molecule inhibitors of amyloidogenic processes. We examined the inhibitory effects of echinacoside (ECH) with different concentrations and at different fiber-forming stages in vitro utilizing the hen egg-white lysozyme (HEWL) model system. We also evaluated the antioxidant capacity of ECH by using elimination tests for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (HO) free radicals. We investigated the protection provided by ECH against neurotoxicity induced by β-amyloid protein (Aβ). Through spectroscopic analyses, electron microscopy, cell viability assay, and hemolysis assay, we found that ECH dose dependently inhibited HEWL aggregation, and this inhibition occurred in different fiber-forming stages. ECH could also scavenge the DPPH and OH free radicals in a concentration-dependent manner. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2',7'-dichlorodihydrofluoresceindiacetate (DCFH-DA) fluorescent measurement results indicated that ECH could increase viability of rat pheochromocytoma PC12 cells injured by Aβ and suppress the increase in intracellular reactive oxygen species (ROS) triggered by Aβ. The present study findings facilitate a better understanding of the interaction between ECH and amyloid-forming proteins and also shed light on the protection of ECH against amyloid fibril-induced neuronal cell death. © 2014 Elsevier B.V.


He R.,Shaanxi University of Chinese Medicine | Liu Q.,Shaanxi University of Chinese Medicine | Liu Y.-H.,Shaanxi University of Chinese Medicine | Chai J.,Shaanxi University of Chinese Medicine | And 10 more authors.
Zhongguo Zhongyao Zazhi | Year: 2014

Seven compounds were isolated from the leaves of Panax japonicus var. major by chromatographic methods including silica gel, Sephadex LH-20, ODS and semi-preparative HPLC. Their structures were elucidated by their physical and chemical properties and spectral data analysis as 5, 7-dihydroxy-8-methoxyl flavone(1), ginsenoside Rs2(2), quinquenoside R1(3), ginsenoside Rs1(4), notoginsenoside Fe(5), ginsenoside Rd2(6) and gypenosiden IX(7). Among them, compound 1 was obtained from the Panax genus for the first time, and compounds 2-7 were isolated from this plant for the first time.


Duan L.,PLA Fourth Military Medical University | Duan L.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Duan L.,Chinese People's Liberation Army | Li H.,PLA Fourth Military Medical University | And 19 more authors.
Analytical Methods | Year: 2015

In this study, a novel, convenient, accurate, and valid method was developed by using high-performance liquid chromatography-photodiode array detection to obtain a chromatographic fingerprint of Antike capsule (AC). Using computer aided similarity evaluation software, 28 characteristic peaks in chromatograms of 10 batches of analyzed samples were screened out and traced to the source of original materials, toad skin and angelica, in which 16 of the peaks were identified as gamabufotalin, arenobufagin, telocinobufagin, desacetylcinobufotalin, bufotalin, cinobufotalin, bufalin, cinobufagin, resibufogenin, ferulic acid, n-butylidenephthalide, senkyunolide A, senkyunolide I, senkyunolide H, ligustilide, and coniferylferulate. At the same time, the fingerprint similarity was calculated and the contents of known ingredients were also determined simultaneously. This method demonstrated good precision, reproducibility, and stability (relative standard deviation [RSD] of relative retention time [RRT] < 2.0% and RSD of relative peak area [RPA] < 5.0%). Good linear behaviors over the investigated concentration ranges were observed for all the analytes (r2 > 0.9994), and the recoveries and RSD varied from 96.35% to 102.43% and 0.48% to 1.98%, respectively. The proposed method enabled fingerprint analysis and simultaneous identification and determination of 16 constituents in a single run. In addition, it provides a significant reference for the quality control of AC. © 2015 The Royal Society of Chemistry.


Zhang X.-P.,China Pharmaceutical University | Zhang X.-P.,PLA Fourth Military Medical University | Zhang X.-P.,Collaborative Innovation Center for Chinese Medicine in Qinba Mountains | Qin H.,China Pharmaceutical University | And 13 more authors.
Zhongguo Zhongyao Zazhi | Year: 2014

Ten compounds were isolated from the barks of Jasminum giraldii by means of various of chromatographic techniques such as silica gel, Sephadex LH-20 and Rp-HPLC. Their structures were identified by spectroscopic data analysis as (+)-medioresinol(1), (+)-syringaresinol(2), syringaresinol-4'-O-β-D-glucopyranoside(3), oleanic acid(4), 3-methoxy-4-hydroxy-trans-cinnamaldehyde(5), trans-sinapaldehyde(6), syringaldehyde(7), 1-(4-methoxy -phenyl)-ethanol(8), trans-cinnamic acid(9), and 4-(1-methoxyethyl)-phenol(10). Among them, compounds 1-3, 5-8 and 10 were isolated from the J. genus for the first time and compounds 4 and 9 were obtained from J. giraldii for the first time. In the DPPH free radical scavenging assay, compound 1 exhibited significant activity(IC50 55.1 μmol·L-1), compared with vitamin C(IC50 59.9 μmol·L-1); and compound 2 showed moderate activity(IC50 79.0 μmol·L-1), compared with 2, 6-di-tert-butyl-4-methylphenol(IC50 236 μmol·L-1).

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