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Bucharest, Romania

Innocenti A.,University of Florence | Leewattanapasuk W.,University of Kent | Manole G.,Colentina Hospital | Scozzafava A.,University of Florence | And 2 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2010

The protein encoded by the NCE103 gene of Candida glabrata, a β-carbonic anhydrase (CA, EC 4.2.1.1) designated as CgCA, was investigated for its activation with amines and amino acids. CgCA was weakly activated by amino acids such as l-/d-His, l-Phe, l-DOPA, and l-Trp and by histamine or dopamine (KAs of 21.2-37 μM) but more effectively activated by d-Phe, d-DOPA, d-Trp as well as serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (KAs of 10.1-16.7 μM). The best activators were l-/d-Tyr, with activation constants of 7.1-9.5 μM. This study may bring a better understanding of the catalytic/activation mechanisms of β-CAs from pathogenic fungi. © 2010 Elsevier Ltd. All rights reserved. Source


Paraschiv S.,National Diagnostics | Otelea D.,National Diagnostics | Batan I.,National Diagnostics | Baicus C.,Colentina Hospital | And 3 more authors.
Infection, Genetics and Evolution | Year: 2012

HIV-1 subtype B is predominant in Europe except in some countries from Eastern Europe which are characterized by a high prevalence of non-B subtypes and circulating recombinant forms (CRFs). Romania is a particular case: the HIV-1 epidemic started with subtype F1 which is still the most prevalent. Previous studies have shown an increasing prevalence of subtype B which is the second most frequent one among the newly diagnosed individuals, followed by subtype C and several CRFs as well as unique recombinant forms (URFs). Our objective was to analyze in detail the characteristics (way of dispersal, association with transmission risk groups) of the subtype B infections in Romania by means of phylogenetic analysis. Among all the individuals sampled during 2003-2010, 71 out of 1127 patients (6.3%) have been identified to be infected with subtype B strains. The most frequent route of infection identified in HIV-1 subtype B patients in Romania was MSM transmission (39.6%), followed by the heterosexual route (35.2%). Many of the patients acquired the infection abroad, mainly in Western European countries. Phylogenetic analysis indicated the existence of a local transmission network (monophyletic clade) including 14 patients, mainly MSM living in the Bucharest area. We estimate the origin of the local transmission network that dates at the beginning of the 90s; the introduction of the F1 and C subtypes occurred earlier. The rest of the sequences were intermixed with reference strains sampled across Europe suggesting that single infection were not followed by subsequent dispersal within the local population. Although HIV-1 subtype B epidemic in Romania is recent, there is evidence for local spread among the MSMs, in addition to multiple introductions. © 2012 Elsevier B.V. Source


Dobritoiu A.M.,Carol Davila University of Medicine and Pharmacy | Forsea D.G.,Colentina Hospital
Therapeutics, Pharmacology and Clinical Toxicology | Year: 2011

Statins and are by far the most widely used class of lipid-lowering drugs and they are the class of choice for LDL-C reduction. Their cutaneous side effects reported in medical literature so far can be classified into four types: autoimmune reactions, reactions due to dryness of the skin, severe reactions like -DRESS, and allergic reactions. Recent research has proven that statins also have immunomodulatory activities and thus they can be used in the treatment of several immunological conditions which are characterized by a Th1 immune response. Statins are reported to be effective against psoriasis, to improve altered wound repair in diabetic skin, to decrease the incidence of skin cancer or to decrease fibroblasts activities in systemic sclerosis. The recently reported beneficial properties need further investigations. © reserved 2011. Source


Voiosu T.,Colentina Hospital
Romanian journal of internal medicine = Revue roumaine de médecine interne | Year: 2012

Gastrointestinal stromal tumors (GISTs) have been a topic of increasing interest since the discovery of their cellular activation pathway via the receptor for tyrosine kinase (KIT) leading to the possibility of targeted molecular therapy in the form of imatinib mesylate. Endoscopic diagnostic and therapeutic possibilities have also been developing at a rapid pace in recent years. Endoscopic ultrasonography (EUS) allows for an accurate assessment of submucosal tumors and can provide tissue samples for diagnostic purposes using fine needle aspiration techniques. Several newer endoscopic techniques, including contrast enhanced EUS and endoscopic submucosal dissection, have also proven useful in the management of GISTs. Although the many recent studies have focused on the role of endoscopy in diagnosing and treating GISTs, we still need better evidence in order to formulate accurate guidelines. Source


Raicu A.,Kettering General Hospital | Sarbu V.,County Emergency Hospital | Grigore C.,Colentina Hospital | Corbisier C.,Grand Hospital de Charleroi
Archives of the Balkan Medical Union | Year: 2013

Background: Today, bariatric surgery has become a successful therapeutic options in the treatment of morbid obesity. Laparoscopic Adjustable Gastric Band is one of the three techniques that are usually used in all the Bariatric Centers. However, band slippage is a common complication, and different techniques have been tried since the beginnings to reduce the number of this complication. We report a new technique used between 2006-2008 (PFT - pars flaccida technique, with the use of suture between lesser curvature and right crus), and compare slippage rates between literature and this technique. Methods: 86 patients were operated between 2006-2008, using this technique. Data collection was performed prospectively, and a retrospective review (a 3-5 years follow-up study) was undertaken. Results: The follow-up rate was 95,34% (n=82). The incidence of postoperative complications for slippage was 1,16% (n= 1) (comparing to 6 % between 1998-2006 - the rates of the same surgeon before using this technique), for band migration was 0%, for pouch dilatation was 4,65 (n=4), band removal 0%, port dislocation was 6,97 (n=6) and 1,16 (n=l) conversion to LRYGBP at 2 years after operation Conclusion: Band slippage is usually not a rare complication after LASGB, but the use of this technique reduce the rates of operative morbidity. Source

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