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Rijeka, Croatia

Zivkovic J.,University of Nis | Zekovic Z.,University of Novi Sad | Mujic I.,Colegium Fluminense | Vidovic S.,University of Novi Sad | And 4 more authors.
Czech Journal of Food Sciences | Year: 2010

The superoxide radical scavenging and antimicrobial activity were examined of sweet chestnuts: seeds, peeled chestnut, brown seed coat, leaves, catkin, spiny burs, and chestnut bark as well as of Lovran's marrone leaves and grafted Italian marrone cultivar. Parts of chestnut were extracted under the same conditions with 50% ethanol as extractant and afterwards the dry extracts of the examined samples were obtained. The total phenolics and flavonoids contents were determined using standard spectrophotometrical methods. The capacity of the investigated extracts to remove radicals was evaluated by EPR method. The most efficient in scavenging "O2" radicals proved to be the extracts of leaves of grafted Italian marrone (RI = 86%) and of Lovran's marrone cultivar (RI = 80%). Catkin, leaves, chestnut bark, and spiny burs extracts demonstrated the highest antimicrobial activity. Very significant and significant correlations were established between the antimicrobial activity of extracts and 'O2" radicals scavenging in all samples examined. The extracts of Castanea Sativa Mill, are important sources of components active in reducing the level of oxidative stress. Source


Mujic I.,Colegium Fluminense | Dudas S.,Colegium Fluminense | Zekovic Z.,University of Novi Sad | Lepojevic Z.,University of Novi Sad | And 3 more authors.
Acta Horticulturae | Year: 2012

The fig tree (Ficus carica L., Moraceae) is very common in the Mediterranean and in countries with dry and warm-temperate climate. Since ancient times the figs have been used for human consumption. Their nutritive and pharmacological properties have been investigated recently. Fig consumption helps in the prevention of vein blockage, high content in fibers has laxative effects, and the latex inhibits the growth of carcinoma cells. The aim of this study was to determine a content of some active components found in extracts of five different figs cultivars ('Šaraguja', 'Termenjača', 'Crnica', 'Bjelica' and 'Bružetka bijela'). Bioactive compounds were extracted from freezedried fig fruits with 70% methanol or 70% ethanol. Analysis of fig extracts included determination of total phenols and flavonoids, as it was determined that effects on health derived from these compounds. For determination of antioxidant activity, scavenging capacity of the DPPH* radicals and reducing power were determined. Antioxidant compounds were detected and quantified by high performance liquid chromatography (HPLC). Total phenolics content in F. carica extracts varied from 7.24 to 11.17 mg GAE/g of dry extract. All methanolic extracts showed higher content of total phenols. The DPPH radical scavenging capacity was found to exhibit IC50 value for the extract concentration lower than 0.40 mg/ml for extract cultivars 'Crnica', while for others this capacity was higher than 0.60 mg/ml. Using reducing power antioxidant test, higher antioxidant activity was measuered for 'Bjelica' than for all other extracts. Results obtained by reducing power method were compared to activity of ascorbic acid, a strong antioxidant compound. Source


Mujic I.,Colegium Fluminense | Dudas S.,Colegium Fluminense | Skutin H.M.,Colegium Fluminense | Perusic D.,Colegium Fluminense | And 6 more authors.
Acta Horticulturae | Year: 2012

The fig tree (Ficus carica L.; Moraceae) is very common in the Mediterranean and in countries with dry and warm-temperate climates. Since ancient times figs have been used only for human consumption. Their nutritive and pharmacological properties have been investigated recently. Their consumption helps in the prevention of artery blockage, high fiber content has laxative effects, and the fig latex inhibits the growth of carcinoma cells. The aim of this study was to determine the content of some active components contained in extracts of five different fig cultivars ('Šaraguja', 'Termenjača', 'Crnica', 'Bjelica' and 'Bružetka bijela'). Fruit samples were freeze-dried. Extracts of total phenols and flavonoids were obtained by extraction in 70% methanol or 70% ethanol. For determination of antioxidant activity, scavenging capacity on DPPHß radicals and reducing power were performed. By high performance liquid chromatography (HPLC) some antioxidant compounds were detected and quantified. Total phenolics content in F. carica extracts was from 7.24 to 11.17 mg CAE/g of dry extract. All methanolic extracts showed higher content of total phenols. The DPPH radical scavenging capacity was found to exhibit IC50 value for the extract concentration lower than 0.40 mg/ml for extract cultivars 'Crnica', while for others this capacity was higher than 0.60 mg/ml. Using the reducing power antioxidant test, higher antioxidant activity was determined for 'Bjelica' than in all other extracts. Results obtained by the reducing power method were compared to activity of ascorbic acid, standard antioxidant compound. Source

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