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Boyadzhieva I.P.,Bulgarian Academy of Science | Atanasova M.,Bulgarian Academy of Science | Atanasova M.,Crta Colegio Universitario 16 | Emanuilova E.,Bulgarian Academy of Science
Biotechnology Letters | Year: 2010

A new, thermostable superoxide dismutase (SOD) from Bacillus licheniformis M20, isolated from Bulgarian mineral springs, was purified 11-fold with 11% recovery of activity. From native PAGE and SDS-PAGE, the enzyme was composed of two subunits of 21. 5 kDa each. The SOD was inhibited only by NaN3, which suggested that this SOD is of the manganese superoxide dismutase type. The purified enzyme had maximum activity at pH 8 and 55°C. The half-life of the SOD was 10 min at 95°C. © 2010 Springer Science+Business Media B.V. Source

Gonzalez E.G.,Complutense University of Madrid | Krey G.,Greek National Agricultural Research Foundation | Espineira M.,Crta Colegio Universitario 16 | Diez A.,Complutense University of Madrid | And 2 more authors.
Journal of Proteome Research | Year: 2010

We report the novel use of proteomics to investigate protein variation among populations of the European hake (Merluccius merluccius). The liver and brain extracts of 18 hake (N = 36) captured in the Mediterranean Sea, Cantabrian Sea, and Atlantic Ocean were examined by 2D/DIGE and mass spectrometry. Significant differences in protein expression among populations were revealed by 84 spots obtained in the gels for the liver and 145 spots for the brain. Population groups of samples were defined by multivariate analysis (PCA and hierarchical clustering). According to protein expression levels and the functions of the 55 candidate protein spots identified, which showed significant expression differences, highest population discrimination was rendered by brain proteins involved in cell signaling and metabolism/energy and by liver proteins involved in protein fate. Finally, we present a statistically robust framework to accurately classify individuals according to their population of origin. Thus, purposely identified protein isoforms were found to be competent at discriminating populations. These results suggest the possibility of identifying protein biomarkers related to environmental changes in a nonmodel species such as the hake and pave the way for more extensive research on protein variation among populations of marine fishes. © 2010 American Chemical Society. Source

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