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Murviel-lès-Montpellier, France

Couturaud B.,Charles Gerhardt Institute | Bondia A.M.,Montpellier University | Faye C.,COLCOM | Garrelly L.,COLCOM | And 2 more authors.
Journal of Colloid and Interface Science

The present work describes a new environmental friendly strategy for the development of surfaces with high amine density via the grafting of native or modified poly-L-lysine dendrigraft (DGL G3) onto plasma activated polypropylene (PP), polystyrene (PS), polyimide, and polytetrafluoroethylene (PTFE) surface. Modified DGL G3 was prepared by replacement of few peripheral amines by various functionalities. Grafting efficiency was determined by wettability measurements, IRTF, XPS, AFM, and by colorimetry using optimized Coomassie Brilliant Blue method tailored for surface analysis. It was shown that a 4-7 nm DGL G3 monolayer with 4 × 1014 amine cm-2 was covalently grafted onto various surfaces. Immobilization of adenosine triphosphate on the DGL-g-PP material from dilute solution was studied by bioluminescence and proved the ability of the material to interact with polyanionic biological compounds: 1 ATP complex with 5 amine groups. So, this material has a potential use in diagnostic and more widely for biotechnology due to its high capacity for biomolecule immobilization. © 2013 Elsevier Inc. Source

Jebors S.,Max Mousseron Institute of Biomolecules | Cecillon S.,Ecole Centrale Lyon | Faye C.,COLCOM | Enjalbal C.,Max Mousseron Institute of Biomolecules | And 4 more authors.
Journal of Materials Chemistry B

A straightforward method for the preparation of hybrid bioorganic-inorganic materials is reported. Common strategies to synthesize such promising materials require special surface modifications of silica followed by grafting of the organic moiety via chemoselective ligation. In this context, we set up a general and bottom-up strategy relying on modified peptides functionalized with a trialkoxysilane group. Used in mixtures with TEOS and a surfactant as the structure directing agent, these hybrid building blocks allow one step direct synthesis of bioorganic-inorganic hybrid materials. Two examples were chosen to demonstrate our general approach. (1) An antifouling surface was prepared by dip coating of a sol containing an antibacterial silylated peptide. (2) Organized mesoporous silica displaying a peptide catalyst in the pores was prepared in one step and tested. © 2013 The Royal Society of Chemistry. Source

Coussot G.,Max Mousseron Institute of Biomolecules | Faye C.,Max Mousseron Institute of Biomolecules | Ibrahim A.,Max Mousseron Institute of Biomolecules | Ramonda M.,Montpellier University | And 6 more authors.
Analytical and Bioanalytical Chemistry

The functionalization of surfaces with amino groups is used in many application areas such as in industrial biocatalytic processes for the development of medical biomaterials and in the environment for removing pollutants from water. Amino group density and grafting stability are often related to functionalized material performances; thus, their characterizations are of prime importance. The determination of amino density and grafting stability on polymeric material (e.g. polypropylene, polystyrene and cylco olefin copolymer) is often time consuming and sometimes presents technical constraints, more particularly with non-flat materials. In this paper, we report a novel colorimetric assay using the Coomassie Brilliant Blue dye for both amino density determination and grafting stability measurement. The assay named ADECA for "Amino Density Estimation by Colorimetric Assay" is sensitive, rapid, robust and versatile. We demonstrate that ADECA makes the evaluation of aminated materials performances possible for numerous material compositions, formats and chemistries used for grafting. Our study focuses on dendrigraft of poly-l-lysine and poly(amidoamine) dendrimers dendritic materials. © Springer-Verlag 2011. Source

Ibrahim A.,Montpellier University | Koval D.,Czech Institute of Organic Chemistry And Biochemistry | Kasicka V.,Czech Institute of Organic Chemistry And Biochemistry | Faye C.,COLCOM | Cottet H.,Montpellier University

In this work, capillary isotachophoresis (ITP) was used to determine the effective charge of the first five generations of dendrigraft poly-l-lysines. This approach, which is based on the linear dependence of ITP zone length of the solute on its concentration and effective charge, offers a simple and straightforward method for effective charge determination. The cationic ITP system employed in this work yields good linearity, repeatability and sharp zones. The value of effective charge number per one lysine residue obtained for long linear poly-l-lysine is in a good agreement with the Manning theoretical value (0.5). Results obtained for dendrigraft poly-l-lysines show a dramatic decrease in the effective charge number per lysine residue with increasing generation number, from 0.84 for short oligolysines (generation 1) down to 0.08 for the fifth generation. This decrease in effective charge is due to the proximity of charged groups in the dendrigraft structure of higher generation number. © 2012 American Chemical Society. Source

Bondia A.M.,French National Center for Scientific Research | Larcher N.,French National Center for Scientific Research | Garrelly L.,COLCOM | Rossi J.C.,French National Center for Scientific Research | Pascal R.,French National Center for Scientific Research
Tetrahedron Letters

A bifunctional tetraethylene glycol (TEG) linker was prepared and used as an initiator for the synthesis of the first two generations of dendrigraft poly-l-lysines (DGL). The key steps involved the desymmetrization of TEG by introduction of an amine group after activation of a terminal hydroxyl group and of a conveniently protected aminooxy functionality at the other end. Initiation of l-lysine N-carboxyanhydride polymerization by the terminal amine yielded generations 1 and 2 of DGL in which a subsequent functionalization of the aminooxy group by ligation with entities bearing an aldehyde group turned out to be feasible. © 2010 Elsevier Ltd. All rights reserved. Source

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