Joo T.,Kangwon National University |
Sowndhararajan K.,Kangwon National University |
Hong S.,Kangwon National University |
Lee J.,Kangwon National University |
And 3 more authors.
Saudi Journal of Biological Sciences | Year: 2014
This study was designed to isolate and identify a potent inhibitory compound against nitric oxide (NO) production from the stem bark of Ulmus pumila L. Ethyl acetate fraction of hot water extract registered a higher level of total phenolics (756.93mg GAE/g) and also showed strong DPPH (IC50 at 5.6μg/mL) and ABTS (TEAC value 0.9703) radical scavenging activities than other fractions. Crude extract and its fractions significantly decreased nitrite accumulation in LPS-stimulated RAW 264.7 cells indicating that they potentially inhibited the NO production in a concentration dependent manner. Based on higher inhibitory activity, the ethyl acetate fraction was subjected to Sephadex LH-20 column chromatography and yielded seven fractions and all these fractions registered appreciable levels of inhibitory activity on NO production. The most effective fraction F1 was further purified and subjected to 1H, 13C-NMR and mass spectrometry analysis and the compound was identified as icariside E4. The results suggest that the U. pumila extract and the isolated compound icariside E4 effectively inhibited the NO production and may be useful in preventing inflammatory diseases mediated by excessive production of NO. © 2014.
Park S.,Hoseo University |
Park S.Y.,CodeBio Co. |
Kim K.S.,CodeBio Co. |
Seo G.Y.,CodeBio Co. |
Kang S.,Hoseo University
Journal of the Korean Society for Applied Biological Chemistry | Year: 2014
Although rose hip administration reduces pain and stiffness in patients with osteoarthritis, the mechanism of rose hip to alleviate primary symptoms has not been investigated. We examined how two types of Rosa canina, grown in Denmark (I-Flex) and Coesam in Chile, attenuate the osteoarthritis symptoms in male rats with osteoarthritis. Therefore pain-related behaviors were evaluated, and histological changes and cytokine expression in the articular cartilage of right knee induced osteoarthritis were assessed via intra-articular monoiodoacetate (MIA) injection. The right knee induced swollen knee, limping legs, and disproportional weight distribution into the right hind paw, and reduced maximum velocity to run on a treadmill from day 3 after MIA injection. The symptoms were exacerbated up to about 2 weeks and remained steady until day 21. Overall the osteoarthritis symptoms in MIA-injected control rats did not significantly change over the experimental period. After 21 days, bone mineral density in right leg and knee had greatly decreased in rats injected with MIA. These symptoms were related to increased expression of matrix metalloprotinase (MMP)-3 and MMP-13 in articular cartilage that degraded collage and elevated the production of TNF-α, IL-1β, and IL-6. However, both types of rose hip markedly attenuated all of the symptoms experienced by the control and overall symptoms from day 3 were improved at day 21. However, some symptoms of osteoarthrit remained and there was no difference between both rose hips for alleviating osteoarthritis symptoms. In conclusion, rose hips from Denmark and Coesam in Chile are potential therapeutic agents for the protection of articular cartilage against progression of osteoarthritis. © 2014 The Korean Society for Applied Biological Chemistry.