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Attakpa E.S.,University Abomey Calavi | Sezan A.,University Abomey Calavi | Seri B.,Cocody University
Acta Endocrinologica | Year: 2013

Background. Peroxisome proliferator- activated receptors (PPAR) α and γ are ligand-activated transcription factors and members of the nuclear hormone receptor superfamily that regulate the metabolism of glucose and lipids. Aim. This study investigated the effects of PPARα deficiency on body weight in wild type and PPAR-αnull mice. Materials and methods. The study was performed on male wild type (WT) mice and homozygous PPAR-αnull (PPARα-knockout) mice of C57BL/6J genetic background. The mRNA expression was quantitatively analyzed by the real time of polymerase chain reaction (RTPCR). Liver TG content was measured by using a commercially available kit. Serum triglyceride (TG) content was measured using enzymatic methods. Serum insulin was determined using an ELISA kit. Serum glucose was determined by the glucose oxidase method using a glucose analyser. Results. Compared with WT, PPAR-αnull mice had high relative adipose tissue weight. These mice exhibited high adiposity state. PPAR-αnull mice also expressed high adiponectin and leptin mRNA levels compared to wild type animals. The PPAR-αnull mice had significantly higher body weight than WT. Hepatic TG and FFA were higher in PPAR- αnull mice as compared to WT animals. PPAR-αnull mice had a high accumulation of TG and FFA in the plasma. Serum insulin concentrations and its pancreatic mRNA transcripts were downregulated in PPAR-αnull mice, suggesting that PPARα gene deletion contributes to low insulin gene transcription. We have reported that PPARα deficiency leads to hypoglycaemia in mice. Conclusion. It is suggested a role of PPARα in obesity-diabetes in mice by studying PPARα-knockout mice. Source

Toure A.,Cocody University | Bahi C.,Cocody University | Ouattara K.,Cocody University | Djama J.A.,Biochemical Laboratory of Pasteur Institute of Cote dIvoire | Coulibaly A.,Cocody University
Journal of Medicinal Plant Research | Year: 2011

The aim of the present study was to compare antifungal activity of various extracts (aqueous, ethanol, ethyl acetate and hexane extracts) of Morinda morindoides (Baker) Milne-Redh (Rubiaceae) and investigate their phytochemical screening in order to determine which components give potency to the extract that show highest antifungal activity. M. morindoides leaves were extracted in water (E taq), ethanol (E eth), ethyl acetate (E ace) and hexane (E hex) and each extract was tested against Candida albicans (yeast) and Trichophyton rubrum (mould). Agar dilution method at serial concentrations ranging from 125 to 1.95 mg/ml was used for the determination of the antifungal parameters- minimum inhibitory concentration (MIC) and concentration producing 50% inhibition (IC50) for these strains. Phytochemical screening of the extracts of leaves of M. morindoides showed the presence of alkaloids, polyphenols, tannins, flavonoids, saponins, quinones and sterols in various concentrations. The ethyl acetate did not contain tannins and quinones. Polyphenols, saponins and quinones were absent in hexane extract. Compared to other extracts (E taq, E eth and E ace) of M. morindoides, hexane extract showed the highest antifungal activity against C. albicans with the MIC of 31.25 mg/ml and IC 50 of 6.17±1.04 mg/ml. The same extract presents the strongest activity against T. rubrum with the MIC of 15.62 mg/ml and IC 50 of 2.68±1.19 mg/ml. Among the extracts of M. morindoides leaves, hexane extracts exerted best antifungal potency against strains which are mostly responsible for superficial mycoses. Thus, this extract may be used for the treatment of dermatomycoses. © 2011 Academic Journals. Source

Die-Kacou H.,Cocody University | Kakou A.,Cocody University | Kamagate M.,Cocody University | Yavo J.-C.,Cocody University | And 4 more authors.
Therapie | Year: 2011

In the pathophysiology of hypertension, the profile hemodynamic is modified by the relation between the increased sodium intake and blood pressure (BP) level. An increased sodium diet is related not only on the amount of fluid volume within the organism but also to the elasticity of the cardiovascular system. In humans, age and salt excess reduced elasticity is linked to BP level and to stiffness material within the vascular wall of larges arteries. Actions of vasoactives hormones such as angiotensin II, antidiuretic hormone, and aldosterone are also linked. The purpose of this article is: (i) to report existing work in Africa relating to "salt and hypertension", (ii) to determine the characteristic of hypertension among black populations, and for epidemiologic study in Ivory Coast, (iii) to determine the various characteristics of hypertension, prevention of cardiovascular risk, and to show usual antihypertensive drugs for reduce rigidity and vascular fibrosis. © 2011 Société Française de Pharmacologie et de Thérapeutique. Source

Abdominal tuberculosis is a relatively frequent extra pulmonary form. The authors report one case where the diagnosis was established by inguinal lymphadenitis. The clinical aspect associated at a woman of 35 years old with positive HIV1, chronic abdominal pain, weight loss, occasional dry cough and left inguinal lymphadenitis. TAP scanner showed abdominal lymphadenitis and pleural pulmonary micro nodules. The diagnosis was revealed by the histological analysis of lymph node biopsy. In endemic area, in front of such symptomatology, we must think firstly to tuberculosis and search peripheric adenitis which can facilitate the diagnosis. © 2013 Springer-Verlag France. Source

Atto V.,Cocody University | Bleyere M.N.,Abobo Adjame University | Konan A.B.,Cocody University | Datte J.Y.,Cocody University | Yapo P.A.,Abobo Adjame University
Pakistan Journal of Nutrition | Year: 2013

In Côte d'Ivoire, very few studies are devoted to the exploration of iron status in adolescents for early screening of iron deficiency and anaemia. This study aims to assess the iron status in adolescents aged 12 to 18 years apparently healthy living in Abidjan (Côte d'Ivoire). It also intended to identify the influence of infectious and inflammatory syndromes on indicators of iron status. Eight hundred and forty seven (847) adolescents enrolled in three municipalities of Abidjan, blood samples were carried out for the determination of biological parameters of iron status. Our study indicated that about three on four adolescents have abnormal iron status. This abnormal iron status is composed of 33.9 of iron deficiency, 30% iron deficiency anaemia, 6% of inflammatory anaemia and 4.7% of inflammatory anaemia associated with iron deficiency. The prevalence of infections and inflammatory disorders is also observed in adolescents. In addition, Pearson coefficients correlation indicated a significantly positive correlation of C Reactive Protein (CRP) with serum ferritin. The main cause of pronounced degradation in iron status is insufficient in the size of iron stores. The problem of the iron metabolism alteration is important in the Côte d'Ivoire in adolescents. © Asian Network for Scientific Information, 2013. Source

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