CNRS Polymers, Biopolymer and Surfaces Laboratory

Rouen, France

CNRS Polymers, Biopolymer and Surfaces Laboratory

Rouen, France
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Yoon E.-J.,Institute Pasteur Paris | Chabane Y.N.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Goussard S.,Institute Pasteur Paris | Snesrud E.,U.S. Army | And 3 more authors.
mBio | Year: 2015

Acinetobacter baumannii is a nosocomial pathogen of increasing importance due to its multiple resistance to antibiotics and ability to survive in the hospital environment linked to its capacity to form biofilms. To fully characterize the contribution of AdeABC, AdeFGH, and AdeIJK resistance-nodulation-cell division (RND)-type efflux systems to acquired and intrinsic resistance, we constructed, from an entirely sequenced susceptible A. baumannii strain, a set of isogenic mutants overexpressing each system following introduction of a point mutation in their cognate regulator or a deletion for the pump by allelic replacement. Pairwise comparison of every derivative with the parental strain indicated that AdeABC and AdeFGH are tightly regulated and contribute to acquisition of antibiotic resistance when overproduced. AdeABC had a broad substrate range, including β-lactams, fluoroquinolones, tetracyclines-tigecycline, macrolides-lincosamides, and chloramphenicol, and conferred clinical resistance to aminoglycosides. Importantly, when combined with enzymatic resistance to carbapenems and aminoglycosides, this pump contributed in a synergistic fashion to the level of resistance of the host. In contrast, AdeIJK was expressed constitutively and was responsible for intrinsic resistance to the same major drug classes as AdeABC as well as antifolates and fusidic acid. Surprisingly, overproduction of AdeABC and AdeIJK altered bacterial membrane composition, resulting in decreased biofilm formation but not motility. Natural transformation and plasmid transfer were diminished in recipients overproducing AdeABC. It thus appears that alteration in the expression of efflux systems leads to multiple changes in the relationship between the host and its environment, in addition to antibiotic resistance. © 15 Yoon et al.


Laloyaux X.,Catholic University of Louvain | Fautre E.,Catholic University of Louvain | Blin T.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Purohit V.,CNRS Polymers, Biopolymer and Surfaces Laboratory | And 4 more authors.
Advanced Materials | Year: 2010

The composition of temperature-responsive copolymer brushes based on oligo(ethylene glycol) methacrylates is tuned to obtain a collapse temperature of ∼35 °C. The chains are grafted by antibacterial magainin I peptide, whose activity is tested at different temperatures against various bacteria. The brushes switch from bactericidal to cell-repellent below and slightly above 35 °C, respectively, due to the progressive vertical collapse of the brush. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Blin T.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Purohit V.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Leprince J.,University of Rouen | Jouenne T.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Glinel K.,Catholic University of Louvain
Biomacromolecules | Year: 2011

Silica and paramagnetic silica microparticles are surface-modified by an antibacterial macromolecular coating. For this, a hydrophilic copolymer brush based on oligo(ethylene glycol) methacrylates is grown on the particle surface by surface-initiated ATRP. Then, Magainin-I, a natural antimicrobial peptide, is grafted onto the hydroxyl groups of the brush through a heterolinker. The grafting of the peptide is evidenced by fluorescence microscopy and X-ray photoelectron spectroscopy. Moreover, culturability and viability assays performed in the presence of the magainin-grafted particles prove their bactericidal properties. The rapid recovery of the bactericidal particles based on paramagnetic silica and suspended in solution is shown under magnetization. Such particles offer the advantage to treat efficiently various sensitive aqueous solutions while avoiding any dissemination of bactericidal substances in the environment. As a consequence, they are of a great interest for various applications in medical, cosmetic, or biomedical fields. © 2011 American Chemical Society.


Glinel K.,Catholic University of Louvain | Thebault P.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Humblot V.,University Pierre and Marie Curie | Pradier C.M.,University Pierre and Marie Curie | Jouenne T.,CNRS Polymers, Biopolymer and Surfaces Laboratory
Acta Biomaterialia | Year: 2012

Prevention of bacterial adhesion and biofilm formation on the surfaces of materials is a topic of major medical and societal importance. Various synthetic approaches based on immobilization or release of bactericidal substances such as metal derivatives, polyammonium salts and antibiotics were extensively explored to produce antibacterial coatings. Although providing encouraging results, these approaches suffer from the use of active agents which may be associated with side-effects such as cytotoxicity, hypersensibility, inflammatory responses or the progressive alarming phenomenon of antibiotic resistance. In addition to these synthetic approaches, living organisms, e.g. animals and plants, have developed fascinating strategies over millions of years to prevent efficiently the colonization of their surfaces by pathogens. These strategies have been recently mimicked to create a new generation of bio-inspired biofilm-resistant surfaces. In this review, we discuss some of these bio-inspired methods devoted to the development of antibiofilm surfaces. We describe the elaboration of antibacterial coatings based on natural bactericidal substances produced by living organisms such as antimicrobial peptides, bacteriolytic enzymes and essential oils. We discuss also the development of layers mimicking algae surfaces and based on anti-quorum-sensing molecules which affect cell-to-cell communication. Finally, we report on very recent strategies directly inspired from marine animal life and based on surface microstructuring. © 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.


Machado I.,IBB Institute for Biotechnology And Bioengineering | Coquet L.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Jouenne T.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Pereira M.O.,IBB Institute for Biotechnology And Bioengineering
Journal of Proteomics | Year: 2013

This study aimed to assess the membrane modifications in Pseudomonas aeruginosa after continuous exposure to increasing doses of benzalkonium chloride (BC). Two different concentrations were used, 0.9 and 12.0. mM.Proteomic investigations revealed that the range of the outer membrane proteome alterations following continuous exposure is very low, i.e. about 10% and BC concentration dependent. Adapted cells revealed different expressions of key proteins frequently reported as involved in acquired resistance mechanisms. Porins (OprF and OprG) and lipoproteins (OprL and OprI) were underexpressed when the higher adaptation concentration (12. mM) was used.Some of these membrane alterations have been described as involved in the acquired resistance to antibiotics, suggesting possible common mechanisms between these two types of resistance. Biological significance: Results obtained after P. aeruginosa adaptation to benzalkonium chloride suggest that the bacterial adaptation to BC do not mobilize complete outer membrane systems. Though, we showed that adaptive resistance to BC promoted some changes in proteins previously described as involved in antibiotic resistance. These results contribute to the assumption that there are common resistance mechanisms, between adaptive and acquired resistance of P. aeruginosa. © 2013 Elsevier Ltd.V.


Delpouve N.,CNRS Laboratory for the Characterization of Amorphous Polymers | Stoclet G.,University of Lille Nord de France | Saiter A.,CNRS Laboratory for the Characterization of Amorphous Polymers | Dargent E.,CNRS Laboratory for the Characterization of Amorphous Polymers | Marais S.,CNRS Polymers, Biopolymer and Surfaces Laboratory
Journal of Physical Chemistry B | Year: 2012

Crystallization is among the easiest ways to improve polymer barrier properties because of the tortuosity increase within the material and the strong coupling between amorphous and crystalline phases. In this work, poly(lactic acid) (PLA) films have undergone α∼ thermal crystallization or different drawing processes. Although no effect of α∼ thermal crystallization on water permeability is observed, the drawing processes lead to an enhancement of the PLA barrier properties. This work clearly shows that, in the case of PLA, the crystallinity degree is not the main parameter governing the barrier properties contrary to the crystalline and amorphous phase organizations which play a key role. X-ray analyses confirm that the macromolecular chain orientation in the amorphous phase is the main cause of the improvement of the drawn PLA water barrier property. This improvement is due to the orthotropic structure formation for sufficient draw ratios, particularly when using the Simultaneous Biaxial drawing mode. Moreover, independently of the draw conditions, the drawing process tends to reduce the plasticization coefficient. Consequently, the drawn material barrier properties are not much affected by the water passage. © 2012 American Chemical Society.


Lenormand H.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Vincent J.-C.,CNRS Polymers, Biopolymer and Surfaces Laboratory
Carbohydrate Polymers | Year: 2011

Hyaluronan (HA) hydrolysis catalysed by hyaluronidase (HAase) is enhanced when bovine serum albumin (BSA) is present and competes with HAase to form electrostatic complexes with HA. At 1 g L -1 HA and BSA concentrations, BSA is able to form three types of complexes with HA depending on pH ranging from 2.5 to 6: insoluble neutral complexes at low pH values, sedimentable slightly charged complexes at pH near 4 and soluble highly charged complexes at pH near 5. The BSA content, charge and solubility of the HA-BSA complexes increase when pH is increased up to the pI of BSA. The normalised charge excess does not exceed 20% for the sedimentable complexes and 40% for the soluble complexes. It has been shown that the sedimentable slightly charged HA-BSA complexes are the most efficient to compete with HAase and release it. All the HA-BSA complexes are hydrolysable by HAase. The HA-BSA binding site shows that one BSA molecule is associated with 85-170 HA carboxyl groups, depending on pH. Similar results have been obtained for lysozyme over an extended pH domain, including the neutrality. © 2011 Elsevier Ltd. All rights reserved.


Alix S.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Lebrun L.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Morvan C.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Marais S.,CNRS Polymers, Biopolymer and Surfaces Laboratory
Composites Science and Technology | Year: 2011

Silane (Si) and styrene (S) treatments were applied on flax fibres in order to improve their adhesion with a polyester resin and to increase their moisture resistance. The water sorption and permeation kinetics of the composites were correlated with the water sorption behaviour of untreated and treated fibres. An increase of the water barrier effect was observed in treated fibres-based composites in comparison with untreated ones. This was related to the shift-down of water solubility and to a decrease of the water diffusivity in treated fibre-based composites. In the case of (S) treatment, the presence of styrene increased the moisture resistance of the treated fibres and made compatible the fibres and the matrix. In the case of (Si) treatment, a good hydric fibre/matrix interface was obtained due to crosslinking reactions and hydrogen bonding between water molecules and free hydroxyl groups of (Si) treated fibres. In order to interpret water permeation behaviour of composite films, a simple illustrated model is suggested and represented by a schematic view. © 2011 Elsevier Ltd.


Hayki N.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Desilles N.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Burel F.,CNRS Polymers, Biopolymer and Surfaces Laboratory
Polymer Chemistry | Year: 2011

For the first time, ethylketene, synthesized by thermolysis of butyric anhydride, was polymerized cationically in order to obtain a novel aliphatic polyketone. Initiated by Lewis acid initiator AlBr3, this polymerization was undertaken in different solvents and at -78 °C (top polymerization yield of 57% in toluene). 1H-13C HSQC NMR experiments clearly demonstrated the polyketonic microstructure. The thermal properties of this new polymer were analyzed by DSC, TGA and X-ray diffraction: with a glass transition temperature around 70 °C and an average crystallinity of 0.34, this polymer showed a starting degradation temperature near 210 °C. © 2011 The Royal Society of Chemistry.


Demay-Drouhard P.,University of Paris 13 | Nehlig E.,University of Paris 13 | Hardouin J.,CNRS Polymers, Biopolymer and Surfaces Laboratory | Motte L.,University of Paris 13 | Guenin E.,University of Paris 13
Chemistry - A European Journal | Year: 2013

A light click away: The first application of the thiol-yne reaction to nanoparticle functionalization is described (see figure). This metal-free click chemistry approach is compatible with the addition of various molecules at the surface and can be combined with CuAAC methodology to perform chemoselective double functionalization. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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