Time filter

Source Type

Gastineau R.,University of Maine, France | Pouvreau J.-B.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | Hellio C.,University of Portsmouth | Morancais M.,University of Nantes | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

Marennine, the blue pigment produced by the diatom Haslea ostrearia, exists in two different forms, the intra- and extracellular forms. We investigated the antibacterial, antiviral, and antiproliferative properties of both of these forms. Both forms of marennine inhibited the development of marine bacteria, in particular the pathogenic organism Vibrio aesturianus, at concentrations as low as 1 μg/mL, but they did not display any effect on a wide range of pathogenic bacteria that are relevant for food safety. Both forms of the pigment produced by H. ostrearia also exhibited antiviral activity against the HSV1 herpes virus, with intra- and extracellular marennine having EC 50 values of 24.0 and 27.0 μg/mL, respectively. These values are 2 orders of magnitude higher than the value for the reference drug, Zovirax. Moreover, both forms of marennine were effective in slowing or inhibiting the proliferation of cancer cells. This study confirms the potential of marennine as a biologically active organic molecule, which could have a protective effect on bivalves, which filter seawater and fix the pigment on their gills. Moreover, marennine could be used in food engineering and chemistry as a natural blue pigment. However, despite that it is eaten and possibly assimilated by green oyster consumers, it also deserves in depth evaluation before being considered for use as a nutraceutical. © 2012 American Chemical Society. Source

Boyer F.-D.,French National Institute for Agricultural Research | Boyer F.-D.,CNRS Natural Product Chemistry Institute | De Saint Germain A.,French National Institute for Agricultural Research | Pouvreau J.-B.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | And 15 more authors.
Molecular Plant | Year: 2014

Strigolactones (SLs) are known not only as plant hormones, but also as rhizosphere signals for establishing symbiotic and parasitic interactions. The design of new specific SL analogs is a challenging goal in understanding the basic plant biology and is also useful to control plant architectures without favoring the development of parasitic plants. Two different molecules (23 (3′-methyl-GR24), 31 (thia-3′-methyl-debranone-like molecule)) already described, and a new one (AR36), for which the synthesis is presented, are biologically compared with the well-known GR24 and the recently identified CISA-1. These different structures emphasize the wide range of parts attached to the D-ring for the bioactivity as a plant hormone. These new compounds possess a common dimethylbutenolide motif but their structure varies in the ABC part of the molecules: 23 has the same ABC part as GR24, while 31 and AR36 carry, respectively, an aromatic ring and an acyclic carbon chain. Detailed information is given for the bioactivity of such derivatives in strigolactone synthesis or in perception mutant plants (pea rms1 and rms4, Arabidopsis max2 and, max4) for different hormonal functions along with their action in the rhizosphere on arbuscular mycorrhizal hyphal growth and parasitic weed germination. © 2013 The Author. Source

Labrousse P.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | Labrousse P.,CNRS Research Group on Water, Soil and Environment | Delmail D.,CNRS Research Group on Water, Soil and Environment | Arnaud M.C.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | And 2 more authors.
Botany | Year: 2010

Orobanche cumana Wallr., a root parasitic angiosperm, causes severe yield losses in Helianthus annuus L. (sunflower) in Europe. Until now, the only effective method of controlling this parasite has been the use of resistant sunflower genotypes. Broomrape resistance is, however, poorly understood even though previous studies have revealed several defence mechanisms. The study of a susceptible (2603) and a resistant (LR1) sunflower genotype in hydroponic co-culture showed that the degree of infection by broomrape is influenced by the concentration of nutrients in the growth medium. For the susceptible genotype, an increase in broomrape necrosis was observed when the nutrient concentration was increased. In the resistant genotype LR1, the rate of infection was reduced by increasing the concentration of mineral nutrients, measured as a decrease in broomrape attachments and a lack of underground stem development. When sunflowers were cultivated in full-strength medium, these findings correlated with a lower 14C incorporation in broomrape and a change in carbon allocation to host plant organs with a reinforced "shoot apex sink strength". Results demonstrated that in controlled conditions, the nutrient concentration directly affects sunflower resistance potential towards broomrape. Source

Gaudin Z.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | Cerveau D.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | Marnet N.,French National Institute for Agricultural Research | Bouchereau A.,CNRS Institute of Genetics, Environment and Plant Protection | And 3 more authors.
Analytical Chemistry | Year: 2014

An AccQ•Tag ultra performance liquid chromatography-photodiode array-electrospray ionization-mass spectrometry (AccQ•Tag-UPLC-PDA-ESI-MS) method is presented here for the fast, robust, and sensitive quantification of 15N isotopologue enrichment of amino acids in biological samples, as for example in the special biotic interaction between the cultivated specie Brassica napus (rapeseed) and the parasitic weed Phelipanche ramosa (broomrape). This method was developed and validated using amino acid standard solutions containing 15N amino acid isotopologues and/or biological unlabeled extracts. Apparatus optimization, limits of detection and quantification, quantification reproducibility, and calculation method of 15N isotopologue enrichment are presented. Using this method, we could demonstrate that young parasite tubercles assimilate inorganic nitrogen as 15N-ammonium when supplied directly through batch incubation but not when supplied by translocation from host root phloem, contrary to 15N2-glutamine. 15N2-glutamine mobility from host roots to parasite tubercles followed by its low metabolism in tubercles suggests that the host-derived glutamine acts as an important nitrogen containing storage compound in the young tubercle of Phelipanche ramosa. © 2013 American Chemical Society. Source

Dongo A.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | Leflon M.,Center Technique Interprofessionnel des Oleagineux Metropolitains | Simier P.,CNRS Nantes Laboratory of Vegetal Biology and Pathology | Delavault P.,CNRS Nantes Laboratory of Vegetal Biology and Pathology
Weed Research | Year: 2012

The objective of this study was to develop a simple and robust quantitative PCR method for the detection of seeds from two parasitic plants, Phelipanche ramosa and Orobanche cumana, as well as their closely related species, in seed harvests of oilseed rape and sunflower. The method was based on the design of primers/probe sets specific to both parasitic plants and targeting internal transcribed spacer sequences for quantitative real-time PCR (TaqMan). Together with the proposed DNA extraction protocol, this diagnostic method allows rapid, high-throughput and accurate assessment of contamination with broomrape seeds, without the requirement of tedious purification steps and identification under a binocular microscope. The TaqMan assay is highly specific, because it did not detect any possible plant contaminants that are likely to be present in harvested crop seeds. The results of this assay can be expressed in terms of the number of parasite seeds per kilogram of crop seeds, a metric that could be utilised to help decisions regarding crop seed lot utilisation and commercialisation. © 2011 The Authors. Weed Research © 2011 European Weed Research Society. Source

Discover hidden collaborations