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Cheongwon gun, South Korea

Lee M.-J.,CNABiotech. Co. | Jeong N.-H.,Cheongju University | Jang B.-S.,CNABiotech. Co.
Journal of Industrial and Engineering Chemistry | Year: 2015

The objectives of this study were to investigate the antioxidant activity and antiaging effect of carrot glycoprotein (hereinafter called as CG), it is compared with the fish scale collagen peptide (hereinafter called as SCP). As a result of analysis of the content of the total flavonoid which is one of ingredients of anti-oxidative action, CG and SCP was 1.35. mg/g and 1.46. mg/g, respectively. In the experiment of oft the radical elimination ability by means of 1,1-diphenyl-2-picryhydrazyl (hereinafter called as DPPH), CG expressed anti-oxidative effect depending on the concentration considering SCP. The Lipid per-oxidation of CG and SCP expressed changes of 9.37%, 36.72%, 51.64% and 0%, 28.93%, 63.84%, respectively, according to the passage of time of 1 day, 3 days and 5 days, so CG and SCP expressed a higher lipid per-oxidation than that of comparison group butylated hydroxyl anisole (hereinafter called as BHA) and vitamin E. In vitro the cytotoxic experiment using the fibroblasts of the dermis of human body, the toxicity didn't appear in the concentration less than CG 1.0. mg/ml, SCP 0.75. mg/ml. In the efficacy experiment to promote the generation of collagen type-1, it appeared that CG promoted the generation of collagen type-1 in more than 0.5. mg/ml concentration and SCP did so in more than 0.3. mg/ml concentration. In the inhibition experiment of matrix metalloproteinase-1 (hereinafter called as MMP-1) expression, CG was reduced meaningfully and statistically all in the concentration of 0.25. mg/ml (p<. 0.05), 0.5. mg/ml (p<. 0.01), 1. mg/ml (p<. 0.01), and SCP showed the inhibition of MMP-1 expression in 0.3. mg/ml (p<. 0.01). These results express that CG eliminates reactive oxygen species (hereinafter called as ROS), protect cell membrane, and can act as antioxidants and antiaging article in the skin exposed to solar ultraviolet light. Therefore, it is thought that CG can be applied to new material related to antioxidants and antiaging. © 2014 The Korean Society of Industrial and Engineering Chemistry.

Lee M.-J.,CNABiotech. Co. | Jang B.-S.,CNABiotech. Co. | Jeong N.-H.,Cheongju University
Applied Chemistry for Engineering | Year: 2013

This study was performed to investigate the physicochemical characteristics of glycoprotein extracted from carrot. We manufactured glycoprotein using natural vegetable raw materials carrots, and then analyzed it's physicochemical properties. The results showed that the nutritional composition of carrot glycoprotein (CG) consisted of 2.35% carbohydrate and 94.2% glycoprotein. There were small amounts of hydroxyproline and glycine known as characteristics of collagen peptide in the amino acid of CG, but there were large amount of glutamic acid and aspartic acid involved in the metabolism of glucose and fat. The calories of CG was 342.1 kcal/100 g and the molecular weight appeared less than 594 Da average molecular weight distribution.

Jang B.-S.,CNABiotech. Co. | Lee M.-J.,CNABiotech. Co. | Jeong N.-H.,Cheongju University | Kim T.-Y.,Chungbuk Provincial College
Applied Chemistry for Engineering | Year: 2013

In this research, the collagen peptide powder from flatfish skin (FSCP) was prepared and compared with commercial collagen peptide powder from tilapia scale (TSCP) in the aspect of physiochemical property. The physical property and nutritional components of FSCP appeared almost similarly to those of TSCP, and also in calorie. No differences in calorie between FSCP and TSCP. Amino acid contents of FSCP for example, aspartic acid, serine, histidine, tyrosine and methionine were higher than those of TSCP. In contrast, the hydroxyproline, proline, alanine contents in FSCP were lower than those in TSCP. Especially, the content of essential amino acid of FSCP, which was 22.74%, was higher than that of TSCP evaluated as 13.64%. In the distribution of molecular weight, FSCP was 1000 Da, which showed a comparatively low distribution compared with TSCP, and in emulsion property and stability both FSCP and TSCP showed an excellent trend.

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