Lezmi S.,Sanofi S.A. |
Rokh N.,Sanofi S.A. |
Saint-Macary G.,Sanofi S.A. |
Pino M.,Sanofi S.A. |
And 5 more authors.
Toxicology | Year: 2013
Retinal toxicity of chloroquine has been known for several years, but the mechanism(s) of toxicity remain controversial; some author support the idea that the binding of chloroquine to melanin pigments in the retinal pigmented epithelium (RPE) play a major toxic role by concentrating the drug in the eye. In our study, 12 albinos Sprague-Dawley (SD) and 12 pigmented Brown Norway (BN) rats were treated orally for 3 months with chloroquine to compare functional and pathological findings. On Flash electroretinograms (ERG) performed in scotopic conditions, similar and progressive (time-dependent) delayed onset and decreased amplitudes of oscillatory potentials (from Day 71) and b-waves (on Day 92) were identified in both BN and SD rats. In both strains, identical morphological changes consisted of neuronal phospholipidosis associated with UV auto-fluorescence without evidence of retinal degeneration and gliosis; the RPE did not show any morphological lesions or autofluorescence. IHC analyses demonstrated a decrease in GABA expression in the inner nuclear layer. In addition, a marked accumulation of synaptic vesicles coupled with a marked disruption of neurofilaments in the optic nerve fibers was identified.In conclusion, ERG observations were very similar to those described in humans. Comparable ERG modifications, histopathology and immunohistochemistry findings were observed in the retina of both rat strains suggesting that melanin pigment is unlikely involved. chloroquine-induced impairment of synaptic vesicle transport, likely related to disruption of neurofilaments was identified and non-previously reported. This new mechanism of toxicity may also be responsible for the burry vision described in humans chronically treated with chloroquine. © 2013 Elsevier Ireland Ltd.
Lazard P.,Clinique Veterinaire |
Peiffer R.L.,Merck And Co.
Revue de Medecine Veterinaire | Year: 2010
A case of anterior segment dysgenesis in an 18-month-old, one-eyed, domestic shorthair cat is reported. This congenital anomaly presented with a central corneal coloboma, a microspheric lens with a partial cataract, pupillary membrane remnants and goniodysgenesis. Treatment consisted of lens removal by phacoemulsification and a corneal graft using a porcine small intestinal submucosa biomaterial (Vet BioSISt) to repair the cornea and preserve the vision. Intraocular pressure remained within the normal range. Features of this case warrant classification as Peters' anomaly, first in humans in 1887 and which consists of defective development of the posterior comea caused by abnormal migration and differentiation of neural crest cells impeded by the late separation of the lens vesicle from the surface ectoderm.
Grall A.,French National Center for Scientific Research |
Grall A.,University of Rennes 1 |
Guaguere E.,Clinique Veterinaire Saint Bernard |
Planchais S.,French National Center for Scientific Research |
And 30 more authors.
Nature Genetics | Year: 2012
Ichthyoses comprise a heterogeneous group of genodermatoses characterized by abnormal desquamation over the whole body, for which the genetic causes of several human forms remain unknown. We used a spontaneous dog model in the golden retriever breed, which is affected by a lamellar ichthyosis resembling human autosomal recessive congenital ichthyoses (ARCI), to carry out a genome-wide association study. We identified a homozygous insertion-deletion (indel) mutation in PNPLA1 that leads to a premature stop codon in all affected golden retriever dogs. We subsequently found one missense and one nonsense mutation in the catalytic domain of human PNPLA1 in six individuals with ARCI from two families. Further experiments highlighted the importance of PNPLA1 in the formation of the epidermal lipid barrier. This study identifies a new gene involved in human ichthyoses and provides insights into the localization and function of this yet uncharacterized member of the PNPLA protein family. © 2012 Nature America, Inc. All rights reserved.
Ruytoor P.,National Veterinary School of Alfort |
Dean E.,Clinique Veterinaire |
Pennant O.,Clinique Veterinaire |
Dorchies P.,National Veterinary School of Toulouse |
And 3 more authors.
Emerging Infectious Diseases | Year: 2010
During 2005-2008, veterinary practitioners reported ocular infection by Thelazia spp. nematodes in 115 dogs and 2 cats in southwestern France. Most cases were detected in Dordogne, particularly in 3 counties with numerous strawberry farms, which may favor development of the fruit fly vector. Animal thelaziosis may lead to emergence of human cases.
Carlotti D.N.,Aquivet Clinique Veterinaire |
Guinot P.,Clinique Veterinaire |
Meissonnier E.,1 rue Camille Desmoulins SA |
Germain P.,Clinique Veterinaire des Hutins
Veterinary Dermatology | Year: 2010
Enzootic dermatophytosis in a shelter with approximately 140 cats was treated according to a protocol combining identification, isolation and treatment of subclinical carrier and affected animals in accordance with a three-area system: healthy animals (no lesions and negative cultures), subclinical carrier animals (no lesions but with positive cultures) and clinically affected animals (lesions and positive cultures). The cats were examined and inspected under a Wood's lamp and had samples taken for fungal culture every 2 weeks. Thirty-three per cent of the cats had a positive fungal culture at the start of the study. Clinically affected animals and carriers were treated with a 0.2% enilconazole lotion (Imaverol®) twice a week and given itraconazole (Itrafungol®) 5 mg/kg SID orally every other week. The environment was treated once a day with a 1% bleach solution and once a week with a 0.6% enilconazole (Clinafarm®) solution. Treated animals were considered cured after two consecutive negative fungal cultures. All cats were cured within 56 days. Prophylactic measures against dermatophytosis were implemented for new arrivals consisting of individual quarantine and the systematic taking of fungal cultures. No relapses were observed based on the fungal cultures taken from the animals and the environment over the first 10 months. © 2009 The Authors. Journal compilation © 2009 ESVD and ACVD.
Baneth G.,Hebrew University |
Sheiner A.,Hebrew University |
Eyal O.,Hebrew University |
Hahn S.,Hebrew University |
And 3 more authors.
Parasites and Vectors | Year: 2013
Background: A Hepatozoon parasite was initially reported from a cat in India in 1908 and named Leucocytozoon felis domestici. Although domestic feline hepatozoonosis has since been recorded from Europe, Africa, Asia and America, its description, classification and pathogenesis have remained vague and the distinction between different species of Hepatozoon infecting domestic and wild carnivores has been unclear. The aim of this study was to carry out a survey on domestic feline hepatozoonosis and characterize it morphologically and genetically. Methods. Hepatozoon sp. DNA was amplified by PCR from the blood of 55 of 152 (36%) surveyed cats in Israel and from all blood samples of an additional 19 cats detected as parasitemic by microscopy during routine hematologic examinations. Hepatozoon sp. forms were also characterized from tissues of naturally infected cats. Results: DNA sequencing determined that all cats were infected with Hepatozoon felis except for two infected by Hepatozoon canis. A significant association (p = 0.00001) was found between outdoor access and H. felis infection. H. felis meronts containing merozoites were characterized morphologically from skeletal muscles, myocardium and lungs of H. felis PCR-positive cat tissues and development from early to mature meront was described. Distinctly-shaped gamonts were observed and measured from the blood of these H. felis infected cats. Two fetuses from H. felis PCR-positive queens were positive by PCR from fetal tissue including the lung and amniotic fluid, suggesting possible transplacental transmission. Genetic analysis indicated that H. felis DNA sequences from Israeli cats clustered together with the H. felis Spain 1 and Spain 2 sequences. These cat H. felis sequences clustered separately from the feline H. canis sequences, which grouped with Israeli and foreign dog H. canis sequences. H. felis clustered distinctly from Hepatozoon spp. of other mammals. Feline hepatozoonosis caused by H. felis is mostly sub-clinical as a high proportion of the population is infected with no apparent overt clinical manifestations. Conclusions: This study aimed to integrate new histopathologic, hematologic, clinical, epidemiological and genetic findings on feline hepatozoonosis and promote the understanding of this infection. The results indicate that feline infection is primarily caused by a morphologically and genetically distinct species, H. felis, which has predilection to infecting muscular tissues, and is highly prevalent in the cat population studied. The lack of previous comprehensively integrated data merits the redescription of this parasite elucidating its parasitological characteristics. © 2013 Baneth et al.; licensee BioMed Central Ltd.
Girard N.,Clinique Veterinaire |
Servet E.,Royal Canin |
Hennet P.,University of Paris Descartes |
Biourge V.,Royal Canin
Journal of Veterinary Dentistry | Year: 2010
It has been suggested that tooth resorption (TR) in cats is associated with vitamin D3 status. The purpose of this study was to evaluate any correlation between serum 25-OH-D concentrations and the prevalence of TR. The healthy adult domestic cats (n=64) of this study had been fed similar premium dry-expanded foods throughout their lives. Serum 25-OH-D was measured, and cats received a single, complete periodontal examination, with periodontal probing of each tooth and exploration of the tooth surface using a dental explorer. A complete set of 10 dental radiographs was taken for each cat. There were 168 TRs diagnosed in 40 of 64 cats (85 were Type 1 TR and 83 were Type 2). The mean serum 25-OH-D concentration was 187.7 ± 87.3 nmol/L. The mean serum 25-OH-D in cats with one or more TR was 164.2 ± 78.8 nmol/L, compared with 226.8 ± 88.2 nmol/L for those without TR (p = 0.14). The mean serum 25-OH-D in the 13 cats with >5 TR was 131.2 ± 49.5 nmol/L, which was significantly less than in cats with no TR (p < 0.05). There was no relationship between TR type and serum 25-OH-D. There was no effect of age or sex on serum 25-OH-D. On the contrary, variations in serum 25-OH-D were observed according to the studied breeds. There was no relationship between TR type and serum 25-OH-D. TR prevalence was greater in cats with lower serum 25-OH-D concentrations. In conclusion, the hypothesis that higher serum 25-OH-D concentrations are associated with a higher prevalence of TR is not supported by this study.
Pin D.,VetAgro Sup |
Guerin-Faublee V.,VetAgro Sup |
Garreau V.,Clinique Veterinaire |
Breysse F.,Center Hospitalier Lyon Sud |
And 3 more authors.
Emerging Infectious Diseases | Year: 2014
Bovine nodular thelitis is a granulomatous dermatitis associated with infection with acid-fast bacteria. To identify the mycobacterium responsible for this infection, we conducted phylogenetic investigations based on partial sequencing of 6 genes. These bacteria were identified as an undescribed Mycobacterium species that was phylogenetically related to M. leprae and M. lepromatosis. © 2014, Centers for Disease Control and Prevention (CDC). All rights reserved.
Dulaurent T.,Center Hospitalier Veterinaire |
Bath A.,Clinique Veterinaire |
Isard P.-F.,Center Hospitalier Veterinaire
Veterinary Ophthalmology | Year: 2014
Objective: To describe a means of converting the Kowa RC-2 camera to a digital version. Procedure: The optic (OP) and the film portions (FP) of the Kowa RC-2 were separated. The lateral rails were unscrewed from the front plate of the FP. The front plate was detached from the FP. The plate was fixed to an anodized aluminum plate (AAP). The rails were screwed back onto the front plate. This system was reattached to the OP by means of the rails. A T2-E mount ring was screwed onto the AAP and fixed to a digital camera body (DCB). A flash sync extension cord was used to connect the flash cable of the Kowa to the DCB. The images obtained with the converted Kowa were inverted horizontally and vertically using image processing software. Results: The images obtained with the converted Kowa RC-2 were identical in format to those using the film version with a circular image surrounded by a black rectangle. The imaged ocular structures were accurately depicted, appearing upside down and reversed on the LCD screen. The DCB was set to manual mode and focus. The shutter speed was initially set to 1/250 s, and the ISO was adjusted based on the ocular structure being photographed. An extra filter had to be placed over the light source to dim the light when imaging reflective fundi. Conclusion: The converted digital version of the Kowa RC-2 as described provides an affordable means of obtaining high-quality images of various ocular structures. © 2014 American College of Veterinary Ophthalmologists.
Meheust P.,Clinique Veterinaire |
Robert R.,Nantes University Hospital Center
Veterinary and Comparative Orthopaedics and Traumatology | Year: 2010
This report describes a partial ventral vertebrectomy and interbody fusion for a progressive hemivertebra disease using a ventral transthoracic approach in a 3.6-monthold male Labrador puppy. The goal of this article is to clarify the potential advantages of this uncommon surgical technique for this condition and to discuss its technical aspects. © Schattauer 2010.