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Sakane N.,Clinical Research Institute | Kotani K.,Clinical Research Institute | Kotani K.,Jichi Medical University | Tsuzaki K.,Clinical Research Institute | And 4 more authors.
Annales d'Endocrinologie | Year: 2014

Objectives: Soy isoflavones have received great attention for their beneficial effects on health and disease, i.e., in patients with diabetes. Equol is a biologically active isoflavone-related metabolite with interindividual differences in its production. The current study investigated the relationship between an equol-producing state and the levels of adipocytokine markers in a prediabetic and diabetic population. Subjects and methods: A total of 79 subjects (34 males/45 females) in a prediabetic or diabetic state recruited from the general population were examined regarding their ability to produce equol using urine samples. Clinical data, such as age, smoking as well as anthropometric and biochemical variables, including body mass index (BMI), lipids, insulin, glucose, hemoglobin A1c, leptin and adiponectin, were recorded. Results: Equol producers exhibited lower leptin and leptin/BMI than non-producers among females. Simple correlation tests and stepwise multiple regression analyses revealed a significant inverse correlation between the leptin/BMI and equol-production. This relationship was not found in males. Conclusions: Female equol producers can have favorable metabolic traits in relation to leptin metabolism in this population. Further studies are needed to confirm these results. © 2014.

Yamaguchi M.,University of Hyogo | Kotani K.,Jichi Medical University | Kotani K.,Clinical Research Institute for Endocrine and Metabolic Disease | Tsuzaki K.,Clinical Research Institute for Endocrine and Metabolic Disease | And 6 more authors.
PLoS ONE | Year: 2015

Background: Clock genes regulate circadian rhythm and are involved in various physiological processes, including digestion. We therefore investigated the association between the CLOCK 3111T/C single nucleotide polymorphism and the Period3 (PER3) variable-number tandem-repeat polymorphism (either 4 or 5 repeats 54 nt in length) with morning gastric motility. Methods: Lifestyle questionnaires and anthropometric measurements were performed with 173 female volunteers (mean age, 19.4 years). Gastric motility, evaluated by electrogastrography (EGG), blood pressure, and heart rate levels were measured at 8:30 a.m. after an overnight fast. For gastric motility, the spectral powers (% normal power) and dominant frequency (DF, peak of the power spectrum) of the EGG were evaluated. The CLOCK and PER3 polymorphisms were determined by polymerase chain reaction (PCR) restriction fragment length polymorphism analysis. Results: Subjects with the CLOCK C allele (T/C or C/C genotypes: n = 59) showed a significantly lower DF (mean, 2.56 cpm) than those with the T/T genotype (n = 114, 2.81 cpm, P < 0.05). Subjects with the longer PER3 allele (PER34/5 or PER35/5 genotypes: n = 65) also showed a significantly lower DF (2.55 cpm) than those with the shorter PER34/4 genotype (n = 108, 2.83 cpm, P < 0.05). Furthermore, subjects with both the T/C or C/C and PER34/5 or PER35/5 genotypes showed a significantly lower DF (2.43 cpm, P < 0.05) than subjects with other combinations of the alleles (T/T and PER34/4 genotype, T/C or C/C and PER34/4 genotypes, and T/T and PER34/5 or PER35/5 genotypes). Conclusions These results suggest that minor polymorphisms of the circadian rhythm genes CLOCK and PER3 may be associated with poor morning gastric motility, and may have a combinatorial effect. The present findings may offer a new viewpoint on the role of circadian rhythm genes on the peripheral circadian systems, including the time-keeping function of the gut. © 2015 Yamaguchi et al.

Kotani K.,Clinical Research Institute for Endocrine and Metabolic Disease | Kotani K.,Jichi Medical University | Sakane N.,Clinical Research Institute for Endocrine and Metabolic Disease
Korean Journal of Laboratory Medicine | Year: 2011

Background: Circulating leptin:adiponectin ratio (L: A) is a potential surrogate marker for cardiometabolic diseases; however, the relationship of the L: A with the occurrence of metabolic syndrome (MetS) has not yet been fully explored in the general Japanese population. Methods: We enrolled 678 Japanese subjects (208 men and 470 women, mean age: 58.8 ± 14.4 [SD] yr; mean body mass index: 23.6 ±3.3 kg/m 2) in this study, and determined their MetS status by using the National Cholesterol Education Program-Adult Treatment Panel (NCEP-ATP) recommendations with minor modifications for the Japanese population. Biochemical markers such as leptin and adiponectin present in blood were measured.The statistical analyses performed were gender-based. Results: The L:A in subjects with MetS was significantly higher than that in subjects without MetS, regardless of gender. The L: A also showed a significant and gradual increase corresponding to the increase in the number of components of MetS present in both the genders (trend P < 0.01 ).The cut-off level of the L: A to detect MetS was 0.59 (sensitivity: 0.72, specificity: 0.70) in men and 1.04 (sensitivity: 0.72, specificity: 0.69) in women. Conclusions:These results suggest that the L: A can serve as a clinically useful marker for detecting MetS characteristics in the general Japanese population.The clinical application of this laboratory index for detecting MetS should be assessed in future studies.

Tsuzaki K.,Clinical Research Institute for Endocrine and Metabolic Disease | Kotani K.,Clinical Research Institute for Endocrine and Metabolic Disease | Kotani K.,Jichi Medical University | Fujiwara S.,Mima City National Health Insurance Koyadaira Clinic | And 2 more authors.
Clinical Laboratory | Year: 2014

Background: While alcohol consumption is associated with levels of high-density lipoprotein (HDL)-cholesterol (HDL-C), a cardiovascular risk marker, HDL size distribution has yet to be characterized in subjects with alcoholic fatty liver disease (AFLD) and non-alcoholic fatty liver disease (NAFLD). Methods: The present study compared HDL subfractional characteristics between subjects with AFLD (36 men, age 61 ± 14) and NAFLD (35 men, age 65 ± 13), recruited during general health check-ups. Serum HDL subfractions were measured with the electrophoretic separation of lipoproteins employing the Lipoprint™ system. Results: The subjects with AFLD had a significantly greater proportion of small-sized HDL part (6.6 ± 5.7%) than those with NAFLD (3.8 ± 4.9%, p = 0.029). Conclusions: More percentages of small-sized HDL part were observed in the subjects with AFLD than in those with NAFLD in Japanese general population. Whether the difference of HDL size is associated with cardiovascular manifestations should be studied further.

Kotani K.,Clinical Research Institute for Endocrine and Metabolic Disease | Kotani K.,Touro University California | Kotani K.,Jichi Medical University | Kimura S.,Showa University | And 3 more authors.
Primary Care Respiratory Journal | Year: 2011

Nasal continuous positive air pressure (nCPAP) treatment may favourably affect serum levels of advanced glycation end products (AGEs) in patients with obstructive sleep apnoea syndrome (OSAS). At baseline, OSAS patients had significantly higher levels of AGEs than controls. Six months after nCPAP initiation, AGEs decreased significantly. nCPAP treatment could lower AGEs in patients with OSAS. © 2011 Primary Care Respiratory Society UK.

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