Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province

Wuhan, China

Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province

Wuhan, China

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Huang S.,Wuhan University | Huang S.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | Chen Z.,Wuhan University | Chen Z.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | And 6 more authors.
Medical Journal of Wuhan University | Year: 2010

Objective: To investigate the influence of cytotoxic T lymphocyte antigen-4 (CTLA-4) monoclonal antibody(anti-CTLA-4 mAb) on the development of dextran sulfate sodium (DSS)-induced acute ulcerative colitis and the expression of IL-17 mRNA of mice. Methods: Twenty four female BalB/c mice were randomly assigned to four groups: normal control group (A), model group (B), anti-CTLA-4 mAb group (C), and isotype antibody control group (D). Animals in Group B,C and D were fed with 5.0% (w/v) DSS solution for 7 days to induce acute intestinal inflammation while those in Group A drunk distilled water. Mice in Group C also received intraperitoneal anti-CTLA-4 mAb injection since the start of DSS exposure. The animals were sacrificed on the 7 th day of the experiment. The disease activity index (DAI), histological score and the myeloperoxidase (MPO) activity were analyzed, and the mucosal mRNA expression of IL-17 was determined by reverse transcriptase-polymerase (RT-PCR). Results: In the end of DSS exposure, the DAI and MPO activity and histological score in group C were significantly increased than group B (all P<0.05). 2 The expression levels of mucosal IL-17 mRNA in group C was significantly higher than that in group B (P<0.05). Conclusion: The anti-CTLA-4 mAb aggravates DSS-induced colitis in mice, and it upragulates the mucosal expression of IL-17 mRNA.


Jiang C.,Wuhan University | Jiang C.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | Jiang C.,Hubei Key Laboratory of Intestinal and Colorectal Diseases | Ding Z.,Wuhan University | And 22 more authors.
Techniques in Coloproctology | Year: 2012

Background Transanal surgery using an endoscopic linear stapler is a recognized, but not widely performed technique for the treatment of obstructed defecation syndrome (ODS). A study of consecutive patients was conducted to evaluate the safety and effectiveness of the technique for the treatment of ODS in Chinese patients. Methods From November 2008 to December 2010, 43 female patients with ODS caused by rectocele and/or rectal intussusception underwent transanal surgery using an endoscopic linear stapler in three Chinese hospitals. Clinical and functional data including the Wexner constipation score and outcome classification were analyzed retrospectively. Results The average duration of surgery was 23 ± 4 min (range 15-30 min). Blood loss was 10 ± 2 ml (range 5-15 ml). The average postoperative hospital stay was 5 days (range 4-6 days). The pathologic findings showed that the specimen contained rectal muscle in all patients. Postoperative complications included 4 patients with transient fecal urgency, 3 patients with anorectal pain, and one patient with mild bleeding from the stapled suture line. Three patients reported minor fecal incontinence (Wexner score less than 3). During a median follow-up of 12 months (range, 3-26 months), the mean constipation score improved from preoperative 13.56 to postoperative 5.07 at 1 year (P<0.05). The outcome at 1 year was excellent in 18 of 43 patients, good in 13, fairly good in 7, and poor in 5. Postoperative defecography was performed in 28 patients. Rectocele disappeared in 15 patients. Rectocele depth was reduced from 34 ± 4 mm preoperatively to 17 ± 3 mm postoperatively (P<0.05). Conclusion The transanal procedure using an endoscopic linear stapler is an easy, safe, and effective option for selected patients with ODS. Long-term prospective, randomized, controlled studies are needed to confirm the advantages of this procedure in comparison with the traditional transanal and stapled transanal rectal resection (STARR) techniques. © Springer-Verlag 2011.


Chen Z.,Central Hospital of Wuhan | Zhang H.,Central Hospital of Wuhan | Xia B.,Wuhan University | Xia B.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | And 5 more authors.
International Journal of Colorectal Disease | Year: 2013

Purpose: Our aims were to evaluate protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene polymorphisms in ulcerative colitis (UC) and explore PTPN22 mRNA levels in colonic biopsies of UC patients in central China. Methods: A total of 165 Chinese UC patients and 300 healthy controls were enrolled in this study. PTPN22 -1123G/C, +1858C/T, and +788G/A polymorphisms were genotyped by PCR-restriction fragment length polymorphism method. PTPN22 mRNA expressions in colonic biopsies and serum C-reactive protein (CRP) levels were determined by quantitative PCR and immunonephelometry, respectively. Results: The frequency of C carrier was higher in UC patients than in healthy controls (66.7 vs. 53.3 %, P = 0.005, odds ratios = 1.75, 95 % CI 1.18-2.60) and associated with extensive colitis (P = 0.029). PTPN22 mRNA levels were elevated in UC patients than in healthy controls (P < 0.001). Among UC patients, PTPN22 mRNA expression levels were higher in biopsies of inflamed colonic tissue compared with noninflamed tissue (P < 0.001) and were correlated with CRP levels (r = 0.578, P < 0.001). PTPN22 mRNA expression levels were elevated in extensive colitis compared to proctitis (P = 0.008) and to left-sided colitis (P = 0.029) and were higher in moderate and severe disease than in mild disease (P = 0.005). Conclusions: Our study showed the potential association between PTPN22 -1123G/C polymorphism and UC in central China. PTPN22 mRNA levels were highly expressed in UC, especially in active disease, and were correlated with CRP levels, disease location, and disease severity in UC patients. © 2013 Springer-Verlag Berlin Heidelberg.


Zhao J.,Wuhan University | Jiang Y.,Wuhan University | Lei Y.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | Zou K.,Huazhong University of Science and Technology | And 4 more authors.
Journal of Gastroenterology and Hepatology (Australia) | Year: 2011

Background and Aim: The aim of the present study was to evaluate the contribution of the dimorphism (MICA-129 val and met) to the genetic susceptibility and functions of ulcerative colitis (UC) in patients in central China. Methods: Genotyping of MICA-129 was performed in 272 consecutive UC patients and 560 age- and sex-matched healthy individuals by using a polymerase chain reaction-sequencing based typing (PCR-SBT) method. A total of 93 patients and 98 healthy individuals serum soluble MICA (sMICA) concentrations were detected by enzyme-linked immunosorbent assay. Results: Both the frequencies of the variant allele (val) and genotype (val/val) in the MICA-129 gene were significantly higher in UC patients than in the controls (77.4% vs 71.7%, P=0.015, 95% confidence interval [CI]: 1.064-1.716; 56.9% vs 46.4%, P=0.005, 95% CI: 1.142-2.047). Serum sMICA levels were significantly higher in UC patients than in the controls (560±140pg/mL vs 157±67pg/mL, P<0.0001). The genotype also affected the extent and the activity of UC. Furthermore, patients with the MICA-129 val/val genotype had higher serum sMICA levels than those with the val/met+met/met genotype (661±352SDpg/mL vs 523±245SDpg/mL, 95% CI: 13.47-265.35, P=0.03). In addition, patients with severe colitis were more susceptible to higher levels of sMICA than those with mild colitis. Conclusions: Our findings showed that the MICA-129 gene polymorphism as a functionally relevant gene was associated with UC and seems to play a potential role in the development of UC in patients in central China. © 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.


Wang Y.,Wuhan University | Jiang C.-Q.,Wuhan University | Jiang C.-Q.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | Jiang C.-Q.,Hubei Key Laboratory of Intestinal and Colorectal Diseases | And 12 more authors.
International Journal of Colorectal Disease | Year: 2013

Background and aims: Molecular testing for epidermal growth factor receptor (EGFR) mutations has recently become a standard practice for the management of patients with non-squamous none small cell lung cancer. Primary small intestine adenocarcinoma (SIA) is an uncommon malignancy, and EGFR mutation in the cancer has not been well characterized due to its rarity. Methods: A micro-tissue array with 53 SIAs and 24 surgically resected primary non-ampullary SIAs were studied. EGFR mutations were analyzed by DNA sequencing in 24 cases with formalin-fixed paraffin-embedded blocks. All 77 cases were examined by immunohistochemistry (IHC) using antibodies specific for the EGFR E746-A750 deletion in exon 19 (DEL), L858R point mutation in exon 21 (L858R), and total EGFR. EGFR amplifications were detected by fluorescence in situ hybridization. Results: A positive reaction of DEL-specific, L858R-specific, and total EGFR antibodies was detected in seven (9.1 %), 5(6.5 %) and 35 (45.5 %) of 77 SIAs by IHC, respectively. Positive reaction of the three antibodies was not significantly correlated with patient's age, gender, differentiation, and stage. EGFR gene amplification was assayed in 77 SIAs in micro-tissue array. Of 24 SIA samples that had DNA sequencing, two (8.3 %) harbored exon 19 deletion and one (4.2 %) harbored L858R point mutation. Only one case with EGFR amplification and two cases with polysomy were shown. Conclusions: Our findings suggested that mutations and amplification in EGFR genes are minor events, and most of SIAs may be unsuitable to EGFR-TKIs treatment. © 2013 Springer-Verlag Berlin Heidelberg.


Chen Z.,Hubei University | Chen Z.,Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province | Brant S.R.,Johns Hopkins University | Li C.,Key Laboratory of Allergy and Immune Related Diseases | And 10 more authors.
Genes and Immunity | Year: 2010

Reduced cytotoxic T-lymphocyte antigen 4 (CTLA4) expression has been proposed as a risk for autoimmunity. CTLA4 polymorphisms have been associated with several autoimmune diseases, including ulcerative colitis (UC). In this study, we performed genotyping for CTLA4 1661A/G, 1722T/C and 3′ untranslated region (AT)n repeat polymorphisms in 300 Chinese UC patients and in 700 healthy controls, and evaluated the effects of polymorphisms on full-length (flCTLA4) and soluble CTLA4 (sCTLA4) expression in UC patients. The frequency of the 1661G allele was higher in UC patients than in healthy controls (16.5 vs 11.4%, P=0.003, odds ratio (OR)1.53, 95% confidence interval (95% CI): 1.17-2.01). The prevalence of (AT)n repeats of the CTLA4 gene carrying long alleles (≥116 bp) was more common in UC patients than in healthy controls (22.0 vs 6.3%, P<0.001, OR4.21, 95% CI: 2.79-6.33), and was associated with extensive colitis (P0.008). Among UC patients, long-allele carriers expressed lower levels of flCTLA4 and sCTLA4 mRNA and sCTLA4 protein than did short-allele carriers (P<0.001, P<0.001, P=0.001, respectively). CTLA4 gene 1661A/G and long 3′ untranslated region (AT)n repeat polymorphisms are associated with UC in Central China. This is likely from decreased expressions of sCTLA4 mRNA and sCTLA4 protein. Our study suggests that CTLA4 has an important role in susceptibility for UC in Central China. © 2010 Macmillan Publishers Limited All rights reserved.


Jiang T.,Wuhan University | Jiang T.,Key Laboratory of Allergy and Immune related Diseases | Ge L.Q.,Wuhan University | Chen Z.T.,Wuhan University | And 8 more authors.
Journal of Digestive Diseases | Year: 2010

Our aim was to investigate the expression of cytotoxic T lymphocyte-associated molecule 4 (CTLA-4) in ulcerative colitis (UC) and to evaluate the effect of CTLA-4 gene -1661A/G polymorphism on CTLA-4 expression and transcription. METHODS: A total of 20 UC patients and 22 healthy controls matched by age and sex were enrolled at Zhongnan Hospital of Wuhan University in central China. The CTLA-4 -1661A/G polymorphism was genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. A Western blot analysis was performed to determine the full length CTLA-4 (flCTLA-4) protein expression in the peripheral blood of the UC patients. Serum-soluble CTLA-4 (sCTLA-4) levels were measured by enzyme-linked immunosorbent assay. CTLA-4-1661G mutant promoter transcription function was analyzed by site-directed PCR-based mutagenesis. RESULTS: CTLA-4 protein expression on CD4+ T cells in UC patients was lower than that in the healthy controls (P < 0.001) while serum sCTLA-4 in the UC patients was significantly higher than that in the healthy controls (P < 0.001). No correlation was found between flCTLA-4 and sCTLA-4 expression levels and the -1661 A/G polymorphism of the CTLA-4 gene. Meanwhile, CTLA-4 -1661 allele A had no significant impact on the promoter activity compared with allele G (P > 0.05). CONCLUSION: CTLA-4 expressions were aberrant in UC patients compared with the healthy controls. CTLA-4 -1661A/G polymorphism had no significant impact on CTLA-4 expression and transcription in the peripheral CD4 T cells of UC patients. © 2010 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Blackwell Publishing Asia Pty Ltd.

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