Laboratory of Clinical Pharmacology

Lausanne, Switzerland

Laboratory of Clinical Pharmacology

Lausanne, Switzerland
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Xia S.,Laboratory of Clinical Pharmacology | Li L.,Shenyang Pharmaceutical University | Li F.,Shenyang Pharmaceutical University | Peng Y.,Shenyang Pharmaceutical University | And 3 more authors.
Acta Chimica Sinica | Year: 2011

Daphnane-type diterpenes showed potent anti-tumour activities, the flower buds of Daphne genkwa which was rich in daphnane-type diterpenes were subject to phytochemical investigation resulting in the isolation of two novel diterpenes (1, 2) and a known diterpene 3, they were elucidated as 12-O-(2′E,4′E-decadienoyl)-4-hydroxyphorbol-13-acetyl (1), isoyuanhuadine (2) and yuanhuadine (3) by spectroscopic data, including 1H NMR, 13C NMR, HSQC, HMBC, NOESY, ESI-MS and HR-ESI-MS. The new compound isoyanhuadine is yuanhuadine's cis-isomer.

Vogne C.,University of Lausanne | Prod'hom G.,University of Lausanne | Jaton K.,University of Lausanne | Decosterd L.A.,Laboratory of Clinical Pharmacology | Greub G.,University of Lausanne
Clinical Microbiology and Infection | Year: 2014

Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS-MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1 h of incubation and both MALDI-TOF and MS-MS approaches exhibited a 100% sensitivity and a 100% specificity. By comparison, molecular genetic techniques (Check-MDR Carba PCR and Check-MDR CT103 microarray) showed a 90.5% sensitivity and a 100% specificity, as two strains of Aeromonas were not detected because their chromosomal carbapenemase is not targeted by probes used in both kits. Altogether, this innovative MALDI-TOF-based approach that uses a stable 10-μg disk of ertapenem was highly efficient in detecting carbapenemase, with a sensitivity higher than that of PCR and microarray. © 2014 European Society of Clinical Microbiology and Infectious Diseases.

PubMed | National Cancer Center
Type: | Journal: Cancer letters | Year: 2016

Metronomic administration of chemotherapy has long been recognized as having a different biological effect from maximal tolerated dose (MTD) administration. Preclinical studies have demonstrated these differences quite elegantly and many clinical trials have also demonstrated reproducible activity albeit small, in varied solid malignancies even in patients who were heavily pretreated. However, the concept of metronomic chemotherapy has been plagued by lack of a clear definition resulting in the published literature that is rather varied and confusing. There is a need for a definition that is mechanism(s)-based allowing metronomics to be distinguished from standard MTD concept. With significant advances made in understanding cancer biology and biotechnology, it is now possible to attain that goal. What is needed is both a concerted effort and adequate funding to work towards it. This is the only way for the oncology community to determine how metronomic chemotherapy fits in the overall cancer management schema.

Jiang C.-M.,Laboratory of Clinical Pharmacology | Li G.-X.,Laboratory of Clinical Pharmacology | Xia S.-X.,Laboratory of Clinical Pharmacology | Tang S.,Laboratory of Clinical Pharmacology | And 3 more authors.
Chinese Journal of New Drugs | Year: 2011

Objective: To study the pharmacokinetics of ginsenoside Rb 1 of Shengmai injection after a single intravenous infusion with constant speed in healthy volunteers. Methods: Fifteen healthy volunteers were administered with a single intravenous infusion of 60 mL Shengmai injection. The plasma concentrations of ginsenoside Rb 1 were determined by LC/MS/MS. The pharmarcokinetic parameters of ginsenoside Rb 1 were calculated by DAS 2.1 software. Results: The plasma concentration-time curve fitted two compartment model. After single intravenous infusion of Shengmai injection, the main pharmacokinetic parameters of ginsenoside Rb 1 were as follow: C max=(10.572 ± 8.952) mg ·L -1, t 1/2=(47.983 ± 7.256) h, AUC 0~144h=(346.668 ± 267.894) mg ·h ·L -1. Conclusion: Administration of a single intravenous infusion of Shengmai injection is safe in healthy volunteers.

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