Tang X.L.,Clinical Laboratory
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences | Year: 2011
To explore the role of neurogenin 3(NGN3) and paired box gene 4(PAX4) in the process of PDX1-driven mesenchymal stem cells (MSCs) to the pancreatic β-cell differentiation. PDX1 gene and NGN3 were constructed with PAX4 genes expression adenovirus vectors, Adxsi-CMV-PDX1 adenovirus infection induced MSCs. One week later, re-Adxsi-CMV-NGN3/CMV-PAX4 adenovirus infection induced MSCs; and detected PDX1, PAX4, NGN3, NK transcription factor related, gene family 2, locus2(NKX2.2), v-maf musculoaponeurotic fibrosarcoma oncogene homolog B(MafB), insulin, glucagon and other pancreatic secretory cell-associated molecule expression. Adxsi-CMV-PDX1 and Adxsi-CMV-NGN3/CMV-PAX4 adenovirus vectors were constructed successfully. Through immuocytochemistry and indirect fluorescence detection, the expressions of PDX1 and NGN3 and PAX4 factors were detected stably in MSCs cellular nuclei which were induced by Adxsi-CMV-PDX1 and Adxsi-CMV-NGN3/CMV-PAX4. After transfection for 5 d, the cells formed round, gathered into a mass and showed bright red with dithizone staining. RT-PCR detection showed NruroD1 and NKX2.2 expression after being induced for 1 week and insulin/proinsulin molecules after being induced for 2 week. The induced cells could express some transcription factors such as NKX2.2 and MafB, and also pancreatic-secreting related molecules such as insulin and glucagon, but not the expressions of MafA and C-peptide. NGN3 and PAX4 have a favourable role in PDX1 inducing mesenchymal stem cells into pancreatic secretory cells.
Coorevits L.,Clinical Laboratory |
Baert F.J.,H. Hart Ziekenhuis Roeselare Menen VZW |
Vanpoucke H.J.M.,Clinical Laboratory HHRM
Clinical Chemistry and Laboratory Medicine | Year: 2013
Background: Faecal calprotectin is a non-invasive marker for neutrophilic intestinal inflammation. It can be used in the differential diagnosis between functional and organic bowel disease. Moreover, it correlates with endoscopic organic bowel disease activity. The objective of this study is to evaluate a recently launched quantitative immunochromatographic point-of-care test: Quantum Blue Calprotectin (B u hlmann Laboratories AG, Sch o nenbuch, Switzerland) in comparison to an established ELISA method (B u hlmann Laboratories AG). Methods: We included 142 samples, either archived ( . 80
Marks V.,University of Surrey |
Wark G.,Clinical Laboratory
Diabetes Research and Clinical Practice | Year: 2013
Insulin or, more appropriately, hypoglycaemia gives rise to a wide variety of interactions with the law. In most cases its role is not seriously open to question occasionally however, it is. This is especially true of situations in which insulin is suspected of having been used inappropriately or maliciously. The major differences between investigation of hypoglycaemia in clinical and forensic situation are that in the latter the history is often unreliable, appropriate samples for analysis were not collected, preserved or labelled correctly and analytical results are likely to be challenged on grounds of specificity, accuracy and interpretation. Immunoassay remains the mainstay of clinical investigation of hypoglycaemia but likely to become displaced by mass-spectrometry in the forensic situation especially now that human insulin is being replaced by synthetic insulin analogues for the treatment of diabetes. © 2013 Elsevier Ireland Ltd.
Dou Y.-H.,Shenzhen University |
Du J.-K.,Shenzhen University |
Liu H.-L.,Shenzhen University |
Shong X.-D.,Clinical Laboratory
Diagnostic Microbiology and Infectious Disease | Year: 2013
We aimed to summarize evidence on the accuracy of procalcitonin (PCT) test in differentiating fungal infection from other causes of infection. We searched electronic database for original researches that reported diagnostic performance of PCT alone or compare with other biomarkers to diagnose invasive fungal infection (IFI). We included 8 qualifying studies studying 474 episodes of suspected fungal infection with 155 (32.7%) probable or proven IFIs. Four studies compared IFI to bacterial sepsis, in which the pooled positive likelihood ratios and negative likelihood ratios were 4.65 (95% confidence interval [CI], 2.46-8.79) and 0.15 (95% CI, 0.05-0.41), respectively. Another 4 studies compared IFI to uninfected individuals, in which the pooled positive likelihood ratios and negative likelihood ratios were 4.01 (95% CI, 2.04-7.88) and 0.23 (0.07-0.77), respectively. The existing literature suggests good diagnostic accuracy for the PCT test for discrimination between IFIs and bacterial infection or noninfectious conditions. Given the high heterogeneity, medical decisions should be based on both PCT test results and clinical findings. © 2013 Elsevier Inc.
Liu J.,Clinical Laboratory
Transplantation Proceedings | Year: 2012
Objective. To evaluate the clinical value of serum cystatin C (ScysC) for the diagnosis of an acute rejection episode after renal transplantation. Methods. The 76 recruited renal transplant patients included 43 without and 33 with an acute rejection episode. We determined the values of serum creatinine (Scr), blood urea nitrogen (BUN), β2 microglobulin (β2-MG), uric acid (UA), and ScysC before surgery and at 1, 3, 5, 7, 14, 30, and 90 days thereafter. The glomerular filteration rate (GFR) was measured by 99mTc-DTPA to evaluate correlations. Results. No significant difference was observed between the groups before surgery (P > .05). In the rejection-free group ScysC was decreased by 48.1% at 1/day after surgery which was greater than the other cohorts. In the rejection group at day 3 the ScysC increased which was earlier than others. The highest coefficient correlation was observed between ScysC and GFR. The AUCROC of ScysC was greater than all of the others. Conclusion. ScysC was a more sensitive marker to detect changes in renal function than Scr, BUN, β2-MG or UA; therefore it can be used to predict an acute rejection episode after transplantation. © 2012 Elsevier Inc.