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Dubrovnik, Croatia

Vlasic V.,Womens and Childrens Hospital | Trifunovic J.,Clinical Hospital Center Osijek | Cepelak I.,University of Zagreb | Nimac P.,Srebrnjak Childrens Hospital
Biochemia Medica | Year: 2011

Background: Urate levels may be a marker of oxidative stress. The aim ofthe present study was to find out are there any differences in urate concentrations in exhaled breath condensate (EBC) between children with obstructive sleep apnea (OSA) and healthy children. Materials and methods: EBC was collected in children with obstructive sleep apnea (OSA) and clinically healthy children. Urate measurements in EBC and serum were performed by enzymatic color test. Results: The higher concentration ofurates in EBC ofchildren with OSA than clinically healthy children indicatethe oxidative stress in their airways. Since there was no significant difference in serum concentration of urates between children with OSA and healthy children, it could be considered that urates are sintetized in the airways of children with OSA. Conclusions: The present study indicated that urates in EBC (but not in serum) may be used as a marker of local synthesis ofantioxidant compounds, but definitive conclusion must be supported by investigations involving larger number of participants. Source

Bosnar D.,University of Zagreb | Elabjer B.K.,University of Zagreb | Busiae M.,University of Zagreb | Roneeviae M.B.,University of Zagreb | And 2 more authors.
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2012

Purpose To evaluate optical ocular components in patients with pseudoexfoliation syndrome using optical low-coherence reflectometry. Methods A prospective cohort study of 224 eyes of patients planned for cataract surgery was conducted in the period from January 2009 until July 2009. Patients were divided in two groups: the first group of 47 eyes with cataract complicated with pseudoexfoliation syndrome and the control group of 177 eyes with uncomplicated cataract. Each group was further divided into two subgroups based on its refractive state: emmetropes and hypermetropes. The optical low-coherence reflectometry biometer LENSTAR® LS 900® was used to define ocular optical components. Results A statistically significant difference of ocular optical components was established between the two groups of patients and its matching subgroups: AL (t= 2.25; p<0.05) and ACD (t=2.24; p<0.05) were significantly higher in the control group, PD was significantly higher in the control group hypermetropes than the PEX group hypermetropes (t=2.21; p<0.05) while LT (t=3.01; p<0.001), AST (t=2.13; p<0.05) and IOL (t=3.06; p<0.001) were higher in the PEX group of patients than in the control group. Conclusions The optical low-coherence reflectometry enabled preoperative detection of zonular weakness and subsequent lens instability documented as a significantly shallower anterior chamber, thicker lens, and smaller pupillary diameter in the pseudoexfoliation syndrome group in the studied population. © Springer-Verlag 2011. Source

Bojic M.,University of Zagreb | Debeljak Z.,University of Zagreb | Debeljak Z.,Clinical Hospital Center Osijek | Marica-Medic-Saric,University of Zagreb | Tomicic M.,Croatian Institute of Transfusion Medicine
Clinical Chemistry and Laboratory Medicine | Year: 2012

Background: Flavonoids are widely distributed across the plant kingdom and are therefore common ingredients in an everyday diet. Some flavonoids have a potential to affect platelet aggregation; most often antiaggregatory effects of flavonoids are observed. The objective of this research was to evaluate the in vitro effect of a selected set of flavonoids on platelet aggregation in whole blood. Methods: The effect of five selected flavonoids (pinocembrin-7- methylether, epicatechin, hesperetin, 6-hydroxyflavone and 3,6-dihydroxyflavone) on platelet aggregation was studied in the citrated whole blood samples collected from 75 healthy volunteers. A Multiplate® impedance analyzer and five different aggregation inducers (ADP, arachidonic acid, collagen, ristocetin and TRAP-6) were utilized for the analysis of samples. Results: Minimal antiaggregatory concentrations (MINaAC) of flavonoids in individual tests were reported in the following ranges: 0.12-1.91 μM; 15.26-244.14 μM; 15.26-122.07 μM; and 0.06-15.26 μM for ADP, collagen, TRAP-6 and ristocetin aggregation-inducers, respectively. When arachidonic acid was used for induction of platelet aggregation, a proaggregatory effect was observed for pinocembrin-7-methylether, epicatechin, hesperetin and 3,6-dihydroxyflavone, while the expected antiaggregatory effect was observed only for 6-hydroxyflavone (MINaAC=7.63 μM). Conclusions: Flavonoids interfere with in vitro platelet aggregation assays exhibiting either anti- or proaggregatory effects in concentration ranges that can be achieved in circulation by dietary intake. Thus, dietary intake of flavonoids should be taken into account when interpreting the results of whole blood platelet aggregation. © 2012 by Walter de Gruyter • Berlin • Boston. Source

Tadzic R.,Gesundheitszentrum Lange Reihe Dr. Tadzic und Kollegen | Mihalj M.,Josip Juraj Strossmayer University of Osijek | Vcev A.,Josip Juraj Strossmayer University of Osijek | Vcev A.,Clinical Hospital Center Osijek | And 3 more authors.
Kidney and Blood Pressure Research | Year: 2013

Background/Aims: To determine the effect of arterial blood pressure (BP) reduction on endocan and soluble cell adhesion molecules' (sCAM) plasma concentration and expression of their ligands on circulatory leukocyte subpopulations. Methods: 24 hypertensive subjects of both sexes (age: 53±8 yrs) were treated with Ca-channel blocker, amlodipin (5-10 mg/day for 8 weeks; to reach BP≤139/89mmHg). The serum sCAMs and endocan concentrations were determined by ELISA kits. Level of ICAM/VCAM ligands on leukocytes was assessed by flow cytometry. Paired t-test, or t-test were used as appropriate, with Pearson's correlation calculated; p<0.05 was considered significant (SigmaPlot v.11). Results: sICAM-1 and sVCAM-1 were decreased (p≤0.001 and p=0.002, respectively), while E-selectin concentration was increased after amlodipin treatment (P=0.014). CD11a/LFA-1 (ICAM-1 and endocan ligand) was significantly increased in all three cell types with BP decrease. CD15 and CD49d/VLA-4 (VCAM-1 ligand) did not change after the treatment. There was significant positive correlation of systolic and diastolic BP with ICAM-1 and VCAM-1, and significant negative correlation of systolic BP with CD11a/LFA-1. Endocan significantly positively correlated with ICAM-1. Conclusions: The increased expression of ICAM/VACM ligands, together with decrease of sCAMs and endocan suggests the de-activation of endothelium with reduction in BP, decreasing the adherence of circulatory leukocytes to endothelium; subsequently decreasing the risk for development of atherosclerosis. Copyright © 2013 S. Karger AG, Basel. Source

Krstacic G.,Institute for cardiovascular diseases and rehabilitation | Parati G.,University of Milan | Gamberger D.,Ruder Boskovic Institute | Castiglioni P.,Don C. Gnocchi Foundation | And 2 more authors.
Medical and Biological Engineering and Computing | Year: 2012

Complexity-based analyses may quantify abnormalities in heart rate variability (HRV). The aim of this study was to investigate the clinical and prognostic significances of dynamic HRV changes in patients with stress-induced cardiomyopathy Takotsubo syndrome (TS) by means of linear and nonlinear analysis. Patients with TS were included in study after complete noninvasive and invasive cardiovascular diagnostic evaluation and compared to an age and gender matched control group of healthy subjects. Series of R-R interval and of ST-T interval values were obtained from 24-h ECG recordings after digital sampling. HRV analysis was performed by 'range rescaled analysis' to determine the Hurst exponent, by detrended fluctuation analysis to quantify fractal longrange correlation properties, and by approximate entropy to assess time-series predictability. Short- and long-term fractal-scaling exponents were significantly higher in patients with TS in acute phases, opposite to lower approximate entropy and Hurst exponent, but all variables normalized in a few weeks. Dynamic HRV analysis allows assessing changes in complexity features of HRV in TS patients during the acute stage, and to monitor recovery after treatment, thus complementing traditional ECG and clinically analysis. © International Federation for Medical and Biological Engineering 2012. Source

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