Clinical and Diagnostic Center

Yekaterinburg, Russia

Clinical and Diagnostic Center

Yekaterinburg, Russia
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Evdokimov V.V.,Federal Res Center For Epid And Micro Named After The Honorary Academy Nf Gamaleya | Naumenko V.A.,Federal Res Center For Epid And Micro Named After The Honorary Academy Nf Gamaleya | Tulenev Yu.A.,Federal Res Center For Epid And Micro Named After The Honorary Academy Nf Gamaleya | Kurilo L.F.,Research Center for Medical Genetics | And 4 more authors.
Voprosy Virusologii | Year: 2016

Infertility is an actual medical and social problem. In 50% of couples it is associated with the male factor and in more than 50% of cases the etiology of the infertility remains insufficiently understood. The goal of this work was to study the prevalence and to perform quantitative analysis of the human herpes viruses (HHV) and high carcinogenic risk papilloma viruses (HR HPV) in males with infertility, as well as to assess the impact of these infections on sperm parameters. Ejaculate samples obtained from 196 males fall into 3 groups. Group 1 included men with the infertility of unknown etiology (n =112); group 2, patients who had female partners with the history of spontaneous abortion (n =63); group 3 (control), healthy men (n = 21). HHV and HR HPV DNA in the ejaculates were detected in a total of 42/196 (21.4%) males: in 31 and 11 patients in groups 1 and 2, respectively (p > 0.05) and in none of healthy males. HHV were detected in 24/42; HR HPV, in 18/42 males (p > 0.05) without significant difference between the groups. Among HR HPV genotypes of the clade A9 in ejaculate were more frequent (14/18, p = 0.04). Comparative analysis of the sperm parameters showed that in the ejaculates of the infected patients sperm motility as well as the number of morphologically normal cells were significantly reduced compared with the healthy men. The quantification of the viral DNA revealed that in 31% of the male ejaculates the viral load was high: >3 Ig10/100000 cells. Conclusion. The detection of HHV and HR HPV in the ejaculate is associated with male infertility. Quantification of the viral DNA in the ejaculate is a useful indicator for monitoring viral infections in infertility and for decision to start therapy.

Bhanushali A.A.,Super Religare Laboratories | Parmar N.,Super Religare Laboratories | Contractor A.,Asian Heart Institute | Shah V.T.,Clinical and Diagnostic Center | Das B.R.,Super Religare Laboratories
Archives of Medical Research | Year: 2011

Background and Aims: Coronary artery disease (CAD) is the leading cause of death worldwide, especially so in Indians. Recently, genome-wide studies have implicated SNPs in the 58 kb region of chromosome 9p21 to be associated with CAD. In the current study we evaluated the association of single nucleotide polymorphism (SNP) rs10757278 at the 9p21 locus with CAD in a population from Western India. Methods: Genotyping for rs10757278 A/G was done by direct sequencing in 215 cases with confirmed CAD and 150 controls. Results: A significantly higher frequency of the G allele was seen in cases as compared to controls (0.64 vs. 0.53). In the current study the G allele showed association with risk of CAD (OR 1.832 per G allele 95% 1.035-3.242, P 0.042; OR 2.452 GG vs. AA 95% 1.358-4.4431, P 0.004). Addition of the 9p21 allele to Framingham risk score (FRS) resulted in a shift of 17% of individuals from the low-risk category to the intermediate-low (>5-<10% 10-year risk) and 7% from intermediate-low to intermediate-high (>10-<20% 10-year risk) categories. Conclusions: The rs10757278 G variant at the 9p21 locus is significantly associated with the risk of CAD in our population of Western India, similar to the observed trend in other populations; however, the association is much stronger in the present cohort and, considering the high propensity of Indians to develop CAD, it is an important marker even in terms of risk classification. © 2011 IMSS.

Katsnelson B.A.,RAS Institute of High Temperature Electrochemistry | Privalova L.I.,RAS Institute of High Temperature Electrochemistry | Sutunkova M.P.,RAS Institute of High Temperature Electrochemistry | Tulakina L.G.,Clinical and Diagnostic Center | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2012

Aqueous suspension of magnetite nanoparticles with primary diameter of 10 nm were intratracheally administered into rat lungs. In 24 h, cells were isolated from bronchoalveolar lavage and examined under a transmission electron microscope. Alveolar macrophages demonstrated ability to actively uptake single nanoparticles and small aggregates composed of such particles, which then formed larger conglomerates inside fused phagosomes. Some of these mature phagosomes shed the membrane and free nanoparticles closely interacted with nuclear membrane and with cristae and mitochondrial membranes thereby inflicting pronounced damage to these intracellular structures. The loss of primary lysosomes can be viewed as indirect evidence attesting to the role played by diffusion of lysosomal hydrolytic enzymes in the final destruction of the alveolar macrophages provoked by nanoparticles. © 2012 Springer Science+Business Media, Inc.

Levitsky G.N.,Clinical And Diagnostic Center < > | Babak S.L.,Moscow State University | Levin O.S.,Moscow Medical Academy | Zverev V.V.,Charitable Fund for LAS patients
Zhurnal Nevrologii i Psihiatrii imeni S.S. Korsakova | Year: 2014

Objective. Lateral amyotrophic sclerosis (LAS) is a very severe neurodegenerative disease with progressive course and terminal respiratory insufficiency. Non-invasive lung ventilation (NLV) is a main method of treatment. We studied the effectiveness of NLV in LAS and assessed patient’s adaptation to NLV, life expectancy, compliance and an effect of NLV on spirometric parameters. Material and methods. NVL was administered to 28 patients, mean age 56.2±9.4 years, including 10 patients with bulbar onset and 16 with spinal onset. The rapid progression of the disease was observed in 15 patients, slow progression in 13 patients. Results and conclusion. NLV used in regime S extended the life of patients, in particular, patients with spinal onset. The adjustment to the NLV device plays a crucial role. Two types of the adjustment were established: gradual (13 patients) and accelerated (4 patients). The causes of the latter were not found. It should be emphasized that several questions related to the details of NVL regime should be specified in further research in the field. © 2014, Media Sphera. All rights reserved.

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