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Bayram F.,Training and Research Hospital | Baskol G.,Biochemistry and Clinical Biochemistry | Tanriverdi F.,Biochemistry and Clinical Biochemistry | Baskol M.,Biochemistry and Clinical Biochemistry | Kocer D.,Erciyes University
Journal of Research in Medical Sciences | Year: 2013

Background: Growth hormone deficiency (GHD) is associated with an increased cardiovascular mortality. Increased oxidative stress has been associated with development of cardiovascular and cerebrovascular diseases. In the present study, we aimed to evaluate oxidant and antioxidant status in patients with GHD by analyzing serum paraoxonase1 (PON1) activity, and malondialdehyde (MDA) and thiol levels. Materials and Methods: This study was a case-control study. Thirty patients with GHD were included in the study and compared with 20 healthy controls. Serum PON1 activity, and MDA and thiol levels were measured according to an enzymatic spectrophotometric method. Results: Serum MDA levels (2.8 ± 1.3 nmol/mL) were higher in GHD group than the controls (1.7 ± 0.5 nmol/mL) (P = 0.001). PON1 activity (149.9 ± 77.9 U/L) was lower in GHD group than the controls (286.3 ± 126.7 U/L) (P = 0.001). Thiol and high-density lipoprotein cholesterol (HDL-cholesterol) levels were lower in GHD group (218.6 ± 103.9 μmol/L and 32.6 ± 13.4 mg/dL, respectively) than the controls (289.6 ± 101.1 μmol/L and 54.3 ± 14.9 mg/dL, respectively) (P = 0.021 and P = 0.001, respectively). In GHD patients, serum MDA level was negatively correlated with serum HDL-cholesterol (r = -0.499, P = 0.001), and serum PON1 activity was positively correlated with serum thiol and HDL-cholesterol levels (r = 0.306, P = 0.032 and r = 0.303, P = 0.033, respectively). Conclusion: These data support that GHD is characterized by an imbalance between oxidant and antioxidant factors. This abnormality may contribute to the increased atherogenic risk in patients with GHD.

Fodor K.,University of Pécs | Tomescova V.,Biochemistry and Clinical Biochemistry | Koszegi T.,University of Pécs | Kron I.,Biochemistry and Clinical Biochemistry | Perjesi P.,University of Pécs
Monatshefte fur Chemie | Year: 2011

UV-vis and fluorescence spectroscopic investigations of chalcones and cyclic chalcone analogues, (E)-2-(4-X-benzylidene)-1-indanones, -tetralones, and -benzosuberones with the same substitution patterns were performed in solvents with different polarity. Comparison of position of the absorption maxima of three substituted series (unsubstituted, methoxy, and dimethylamino) showed the same decreasing order indanones ≥ chalcones ≥ tetralones ≥ benzosuberones in each solvent indicating the strongest conjugation of the rigid, planar indanones. All the compounds showed positive solvatochromism, which is consistent with transitions having significant charge transfer character. The order of the observed solvent-induced bathochromic shifts of the absorption maxima was found to correlate with the donor number of the solvents. The solvent-induced shift of the emission maxima of each compound is larger than that of the absorption maxima. Recording the UV-vis spectra of the compounds in the presence of bovine and human serum albumin resulted in a slight hypsochromic shift of (E)-2-(4-methoxybenzylidene)- and (E)-2-[(4-dimethylamino) benzylidene]benzosuberone indicating changing the polar environment to a less polar one. Such an observation is in accord with an interaction of the molecules with the hydrophobic binding site(s) of the two proteins. © 2011 Springer-Verlag.

Tomeckova V.,Biochemistry and Clinical Biochemistry | Gajova A.,Biochemistry and Clinical Biochemistry | Velika B.,Biochemistry and Clinical Biochemistry | Saxunova L.,Biochemistry and Clinical Biochemistry | Hertelyova Z.,Biochemistry and Clinical Biochemistry
Spectroscopy | Year: 2011

This study was carried out to investigate isolated liver mitochondrial functions after paracetamol administration by monitoring of liver mitochondrial fluorescence properties as well as prooxidative properties of paracetamol. Paracetamol was administered to rat (in subtoxic 500 mg·kg-1 dose) in vivo. The effect of this dose was compared with the subtoxic and toxic dose of paracetamol added to mitochondria in vitro (1 and 1.5) mg paracetamol/mg mitochondrial protein. Subtoxic dose of paracetamol in vitro did not change mitochondrial fluorescence, but it significantly decreased mitochondrial fluorescence in vivo in comparison with control mitochondrial group. Toxic dose of paracetamol in vitro significantly decreased mitochondrial fluorescence. The enzymatic activity of superoxide dismutase (SOD) significantly decreased after paracetamol administration in vitro and in vivo. While both activities of glutathione peroxidase (GPx) and glutathione reductase (GR) significantly increased in dependence upon paracetamol doses. Our experiment showed, that paracetamol participates in formation of free radicals and confirms previous studies, in which paracetamol administration caused elevation of antioxidative enzymes activities in dependence on dose, which is considered therapeutically as subtoxic and toxic. © 2011 - IOS Press and the authors. All rights reserved.

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