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Fernandes C.A.,University of Brasilia | Nobrega Y.K.M.,Laboratory of Clinical Analysis | Nobrega Y.K.M.,University of Brasilia | Tosta C.E.,University of Brasilia
Journal of Alternative and Complementary Medicine | Year: 2012

Objectives: Despite the recognized importance of phagocytes in the maintenance and recovery of health, the influence of meditation on their functions is not properly established. This investigation aimed at evaluating the influence of pranic meditation on the functions of phagocytes, and on the levels of hormones that influence them. Design: A pre-post design was adopted. Setting: The investigation was carried out at a university research laboratory. Subjects and methods: Twenty-nine (29) healthy individuals of both sexes, 24-67 years old (median 45), with no previous experience in meditation, received 3-hour-duration weekly training on pranic meditation during 10 weeks and agreed to engage in daily home practice for 20 minutes. Pranic meditation is a novel method of meditation, based on the Vedic tradition, which uses techniques of breathing and visualization for quieting the mind, and for capturing and intentionally directing prana ("vital energy") wherever necessary. For assessing phagocytosis, the production of hydrogen peroxide and nitric oxide by monocytes, and the concentrations of corticotrophin and cortisol, blood was collected at the beginning (week 1), at the middle (week 5), and by the end (week 10) of the practice period. At the same intervals, melatonin concentrations were evaluated in the saliva. Results: Those who meditated for more than 980 minutes showed increased phagocytosis, their monocytes produced higher concentrations of hydrogen peroxide, and their plasma levels of corticotrophin were reduced. The production of nitric oxide by monocytes, and the levels of cortisol and melatonin were not modified by meditation. Conclusions: This is the first study to show that a short program of pranic meditation practice was able to upregulate the function and metabolism of phagocytes, in parallel with the reduction of the plasma levels of corticotrophin. The results of this study point to a possible causal effect between these events, and indicate that pranic meditation could be useful for stimulating the function and metabolism of phagocytes. © 2012, Mary Ann Liebert, Inc.

Velasco I.,Obstetrics and Gynecology Unit | Santos C.,Laboratory of Clinical Analysis | Limon J.,Obstetrics and Gynecology Unit | Pascual E.,Obstetrics and Gynecology Unit | And 5 more authors.
Annals of Nutrition and Metabolism | Year: 2016

Background/Aims: Human milk is considered the most suitable food for infants. The potential benefits of breastfeeding can be explained by the presence of different growth and neurotrophic factors in human milk. This study was designed to detect some biomarkers in human milk, which could be involved in the infant neurodevelopment and in the regulation of the maturation of neonatal intestine (brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), fibroblast growth factor 21 (FGF21), lysophosphatidic acid (LPA) and autotaxin (ATX)), and compare them on the basis of the consumption of iodine supplements or multivitamins. Methods: A prospective study included 37 healthy breastfeeding mothers, divided into 3 different groups: (1) 10 mothers who did not take supplements, (2) 17 mothers who took potassium iodine (KI) 200 μg/day and (3) 10 mothers who took a multivitamin supplement. Results: The concentrations of BDNF, GDNF, GFAP, FGF21, LPA and ATX in human milk were not significantly different in women who took a multivitamin or KI supplement compared with those who did not take any supplement. Conclusions: The presence of neurotrophic factors in human milk is neither modified by the consumption of supplements nor by their type. © 2016 S. Karger AG, Basel.

Munoz-Colmenero A.,Laboratory of Clinical Analysis | Fernandez-Suarez A.,Alto Guadalquivir Hospital andujar | Fatela-Cantillo D.,Alto Guadalquivir Hospital andujar | Ocana-Perez E.,Laboratory of Immunology | And 2 more authors.
Anticancer Research | Year: 2015

Background/Aim: Tumor M2-pyruvate kinase (M2-PK) is up-regulated in proliferating tissues. It has been shown that tumor M2-PK is detectable and quantifiable in the stool and plasma of patients with colorectal cancer (CRC). Tumor M2-PK has been extensively studied in gastrointestinal tumors but its role in other cancer types has not yet been deeply evaluated. The aim of the study was to determine and compare plasma tumor M2-PK levels in different cancer types. Materials and Methods: All patients undergoing diagnostics for cancer at our Hospital during 2011 were included in the study (n=139). Plasma tumor M2-PK concentration was analyzed by an enzyme-linked immunosorbent assay. Results: The different cancer types found in the study were: 60 colorectal, 43 breast, 8 lung, 5 prostatic, 4 ovarian and the remaining 19 cases were other uncommon tumor types. Most tumors had high concentrations of tumor M2-PK; prostatic, pharyngeal and testicular tumors had levels lower than or near the cut-off. Plasma tumor M2-PK levels were significantly higher in patients with distant metastases and stage IV by TNM. Conclusion: Plasma tumor M2-PK is not a specific marker for CRC and is elevated in many other types of cancers, including breast, lung, ovarian, and thyroid. Small amounts are found in prostatic, pharyngeal and testicular tumors.

Sebastia C.,Laboratory of Clinical Analysis | Soriano J.M.,University of Valencia | Iranzo M.,University of Valencia | Rico H.,University of Valencia
Journal of Food Processing and Preservation | Year: 2010

The effect of three different periods of storage life (0, 15 and 30 days) on the microbiological quality of several foods elaborated with the sous vide cook-chill procedure in a restaurant was studied. These foods are grouped in three categories: meat (breast chicken, foie gras, pork loin and entrecote of veal), fish (codfish, gilt head sea bream, hake and salmon) and vegetable (broccoli, courgette, potato and carrot). Total plate counts in the products studied were in the range of <1.00-3.74 log10 cfu/g. Coagulase negative staphylococci were isolated in the three categories, Enterococcus spp. were isolated from foie gras and Pantoea agglomerans from salmon and carrot. Staphylococcus aureus, Escherichia coli, Salmonella spp., Listeria monocytogenes and Clostridium perfringens were not detected in any of the samples studied. As a consequence of our study, the restaurant has developed and implemented a traceability system to achieve food safety. © 2010 Wiley Periodicals, Inc.

Miranda D.O.,Federal University of Uberlandia | Silva D.A.O.,Federal University of Uberlandia | Fernandes J.F.C.,Federal University of Uberlandia | Queirs M.G.J.,Federal University of Uberlandia | And 7 more authors.
Clinical and Developmental Immunology | Year: 2011

Allergic rhinitis (AR) is a public health problem with high prevalence worldwide. We evaluated levels of specific IgE, IgA, and IgG4 antibodies to the Dermatophagoides pteronyssinus (Dpt) house dust mite and to its major allergens (Der p1 and Der p2) in serum and saliva samples from allergic and nonallergic children. A total of 86 children were analyzed, from which 72 had AR and 14 were nonallergic healthy children. Serum IgE and serum/salivary IgG4 levels to Dpt, Der p1, and Der p2 were higher in allergic children whereas serum/salivary IgA levels to all allergens were higher in nonallergic children. IgE levels positively correlated with IgG4 and IgA to all allergens in allergic children, while IgA levels negatively correlated with IgG4 to Dpt and Der p1 in nonallergic children. In conclusion, mite-specific IgA antibodies predominate in the serum and saliva of nonallergic children whereas mite-specific IgE and IgG4 are prevalent in allergic children. The presence of specific IgA appears to have a key role for the healthy immune response to mucosal allergens. Also, specific IgA measurements in serum and/or saliva may be useful for monitoring activation of tolerance-inducing mechanisms during allergen specific immunotherapeutic procedures, especially sublingual immunotherapy. Copyright © 2011 Diego O. Miranda et al.

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