Oberg H.-H.,University of Kiel |
Sebens S.,Institute for Experimental Medicine |
Krause S.,University of Kiel |
Petrick D.,University of Kiel |
And 8 more authors.
Cancer Research | Year: 2014
The ability of human gd T cells from healthy donors to kill pancreatic ductal adenocarcinoma (PDAC) in vitro and in vivo in immunocompromised mice requires the addition of gd T-cell-stimulating antigens. In this study, we demonstrate that gd T cells isolated from patients with PDAC tumor infiltrates lyse pancreatic tumor cells after selective stimulation with phosphorylated antigens. We determined the absolute numbers of gd T-cell subsets in patient whole blood and applied a real-time cell analyzer to measure their cytotoxic effector function over prolonged time periods. Because phosphorylated antigens did not optimally enhance gd T-cell cytotoxicity, we designed bispecific antibodies that bind CD3 or Vg9 on gd T cells and Her2/neu (ERBB2) expressed by pancreatic tumor cells. Both antibodies enhanced gd T-cell cytotoxicity with the Her2/Vg9 antibody also selectively enhancing release of granzyme B and perforin. Supporting these observations, adoptive transfer of gd T cells with the Her2/Vg9 antibody reduced growth of pancreatic tumors grafted into SCID-Beige immunocompromised mice. Taken together, our results show how bispecific antibodies that selectively recruit gd T cells to tumor antigens expressed by cancer cells illustrate the tractable use of endogenous gd T cells for immunotherapy. © 2013 American Association for Cancer Research.
Segedi L.M.,Clinic of Gynaecology and Obstetrics |
Mandic A.,Oncology Institute of Vojvodina |
Segedi D.,Clinic of Gynaecology and Obstetrics |
Kozarcic T.,Clinic of Gynaecology and Obstetrics
Archive of Oncology | Year: 2011
Ovarian cancer takes the second place in the incidence of all genital carcinomas occurring in pregnancy. In spite of a low overall incidence of ovarian cancer in pregnancy and, in most cases, asymptomatic clinical picture, the routine use of ultrasonographic examination in early pregnancy has led to a more frequent detection of adnexal masses in pregnant women. The authors presented a case of a 35-years-old patient with the diagnosis of unilocular cyst in the left ovary detected by ultrasonography at gestational week 8, with the subsequent sinistral adnexectomy administered for the rupture of the cystic tumor. Based on the histopathological examination we established the diagnosis of a serous cystadenocarcinoma stage I. The patient refused any proposed modality of oncologic diagnostics and therapy, and delivered a healthy female newborn of 3630g/49 cm, 10 days after the probable term of delivery. The control MRI, 8 months post partum did not confirm the presence of any pathological changes of either genital organs or any other organ of the small pelvis. The patient is in good general condition, under continual medical observation. © 2011, Oncology Institute of Vojvodina, Sremska Kamenica.