Cliantha Research Ltd

Ahmadābād, India

Cliantha Research Ltd

Ahmadābād, India
SEARCH FILTERS
Time filter
Source Type

PubMed | Cliantha Research Ltd., Government Dental College & Hospital and GMERS Medical College & Hospital
Type: Journal Article | Journal: Journal of Indian Prosthodontic Society | Year: 2014

There is limited evidence supporting the role of occlusal splints in Temporomandibular disorder (TMD) therapy. The aim of this randomized controlled clinical trial was to assess the efficacy of stabilization splint therapy on TMD related facial pain and mandibular mobility. The sample of study consisted of eighty consecutive patients diagnosed with TMD. Patients were randomly assigned into two groups: a splint group (n=40) comprising of patients treated with stabilization splint, councelling and masticatory muscle exercises, and a control group (n=40), comprising of patients treated with councelling and masticatory muscle exercises alone. Data from both the groups were collected at the beginning of the study and after a 6-month follow up. The outcome variables were visual analogue scale on facial pain intensity and clinical findings for TMD (anterior maximal opening, mandibular right laterotrusion, mandibular left laterotrusion, mandibular protrusion, and number of painful muscle sites). Changes within the splint and control groups (before treatment and 6months after treatment) were analyzed using paired samples t test. Differences in change between the splint and control groups were analyzed using independent samples t-test. The level of significance was set at p<0.05. Facial pain and number of painful muscle sites decreased, and the mandibular mobility increased significantly in both groups after treatment; however the differences in changes in VAS or clinical TMD findings between the two groups were not statistically significant. The findings of this study show that stabilization splint treatment in combination with counselling and masticatory muscle exercises has no additional benefit in relieving facial pain and increasing the mobility of the mandible than counselling and masticatory muscle exercises alone over a 6-months time interval.


Patel D.S.,Pramukh Swami Science And Hd Patel Arts College | Patel D.S.,Cliantha Research Ltd | Sharma N.,Cliantha Research Ltd | Patel M.C.,Pramukh Swami Science And Hd Patel Arts College | And 4 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2012

A rapid, selective and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been proposed for the determination of bumetanide in human plasma using tamsulosin as internal standard (IS). The analyte and IS were extracted from 200 μL of human plasma via solid phase extraction and the chromatographic separation was achieved on Peerless Basic C18 (100. mm × 4.6 mm, 3 μm) column under isocratic conditions. Detection of bumetanide and IS was done by tandem mass spectrometry, operating in positive ionization and multiple reaction monitoring (MRM) acquisition mode. The protonated precursor to product ion transitions monitored for bumetanide and IS were m/ z 365.2 → 240.2 and 409.2 → 228.2 respectively. The method was fully validated as per the US FDA guidelines. The limit of detection and lower limit of quantitation of the method were 0.03 and 0.30. ng/mL respectively with a linear dynamic range of 0.30-200.0. ng/mL for bumetanide. The intra-batch and inter-batch precision (% CV) was ≤6.9% while the mean extraction recovery was >90% across quality control levels. The method is selective in presence of four diuretic drugs and some commonly used medications by healthy volunteers. It was successfully applied to a bioequivalence study of 2. mg bumetanide tablet formulation in 10 healthy Indian male subjects under fasting condition. The reproducibility in the measurement of study data was demonstrated by reanalysis of 42 incurred samples. © 2012 Elsevier B.V.


Patel D.S.,Pramukh Swami Science And Hd Patel Arts College | Patel D.S.,Cliantha Research Ltd | Sharma N.,Cliantha Research Ltd | Patel M.C.,Pramukh Swami Science And Hd Patel Arts College | And 4 more authors.
Journal of Chromatographic Science | Year: 2013

A reliable and sensitive liquid chromatography-tandem mass spectrometry assay has been proposed for the selective determination of diflunisal in the presence of its glucuronide metabolites. The analyte and clofibric acid as internal standard (IS) are extracted from 50 μL of human plasma by solid-phase extraction. Chromatographic separation is conducted on a Prodigy ODS 3V column (150 × 4.6 mm, 5 μm) under isocratic conditions. The possible interference of acyl glucuronide and phenolic glucuronide, the two major inactive metabolites of diflunisal, is also checked in plasma samples. Detection of the analyte and IS is achieved by tandem mass spectrometry, operating in negative ionization and multiple reaction monitoring acquisition mode. The limits of detection and quantitation of the method are 0.10 and 1.00 μg/mL, respectively, with a linear dynamic range of 1.00-160 μg/mL for diflunisal. The intra-batch and inter-batch precision (percent coefficient of variation) is ≤4.2% and the mean recovery is >92% for diflunisal across quality control levels. The method is successfully applied to a bioequivalence study of a 500 mg diflunisal tablet formulation in 30 healthy Indian male subjects under fasting conditions. The reproducibility in the measurement of study data is demonstrated by the reanalysis of 120 incurred samples. © 2012 © The Author [2012]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.


PubMed | Cliantha Research Ltd and GMERS Medical College and Hospital
Type: Journal Article | Journal: Indian journal of dental research : official publication of Indian Society for Dental Research | Year: 2016

There are very few studies concerning the role of denture status in temporomandibular disorders (TMDs) and those show conflicting results.To evaluate the association of edentulousness and removable prosthesis rehabilitation with severity of TMD signs and symptoms.Data were collected from 2000 subjects reporting for dental treatment, above 30 years of age.The subjects were interviewed with a questionnaire and clinically examined for TMD on basis of Helkimos index. The number of existing teeth, wearing of removable dentures, need for denture repair, and age of dentures was recorded.The association between the TMD findings and recorded variable outcomes was analyzed by means of Chi-square test.Completely edentulous individuals associated more with TMD related findings in incidence and intensity than partially/fully dentate subjects. Complete denture wearers were more associated with TMD symptoms, limited mandibular mobility, muscle tenderness, and pain on mandibular movement. Partial denture wearers were more associated with severely impaired temporomandibular joint function and joint pain. Signs and symptoms of TMD were more prevalent and severe in patients who needed to get their dentures repaired, those wearing dentures more than 5-year-old, and in patients who had not got their dentures repaired during the past 5 years.Edentulousness, complete/partial denture wearing and poor condition of the dentures associate with greater incidence and intensity of TMD associated signs and symptoms.

Loading Cliantha Research Ltd collaborators
Loading Cliantha Research Ltd collaborators