Clean Seas Tuna Ltd

Port Lincoln, Australia

Clean Seas Tuna Ltd

Port Lincoln, Australia
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Premachandra H.K.A.,University of The Sunshine Coast | Premachandra H.K.A.,University of Peradeniya | Nguyen N.H.,University of The Sunshine Coast | Miller A.,Clean Seas Tuna Ltd | And 2 more authors.
Aquaculture | Year: 2017

The aquaculture of yellowtail kingfish Seriola lalandi, is now expanding worldwide. Production is conducted in two very different types of commercial facilities: predominately in sea cages in Australia, Mexico, Chile and Hawaii; and in indoor facilities in Europe. With expansion, there is concordant commercial interest to develop genetically improved stocks for production. However, there is a great paucity of genetic information about this species. Knowledge gaps include accurate genetic parameter estimates (i.e., from large sample sizes) that are essential to develop accurate and efficient selection indices. Specifically we require accurate information on non-growth traits (such as parasite load and deformities) and their correlations with growth traits (body weight and fork length), and information on any genotype by environment (G × E) interactions between the two major growing systems to determine whether a single or multiple selection programs are required. Here we address all these fundamental genetic knowledge gaps and provide, for the first time: relatively accurate genetic parameter estimates based on approximately 1400 genotyped and phenotyped animals; and preliminary tests for G × E interactions between growth in sea cages vs land tanks. The heritability estimates were moderate and significant (0.42 ± 0.10) for growth traits, whereas heritability estimates for non-growth traits were low (0.01–0.06). The genetic correlation between body weight and fork length was positive and close to one (0.98); in contrast, the correlation between growth traits with occurrence of fluke and deformity were not significant. The genetic correlations for growth traits between two culture environments were high (0.92–0.97); they were however, associated with large standard errors. In summary, the new set of genetic parameters obtained from this study provide accurate information that is essential to develop genetically improved stocks of yellowtail kingfish for commercial production. © 2017 Elsevier B.V.


Nguyen N.H.,University of The Sunshine Coast | Whatmore P.,University of The Sunshine Coast | Whatmore P.,The Australian Seafood Cooperative Research Center | Miller A.,Clean Seas Tuna Ltd | Knibb W.,University of The Sunshine Coast
Journal of Fish Diseases | Year: 2016

The main aim of this study was to estimate the heritability for four measures of deformity and their genetic associations with growth (body weight and length), carcass (fillet weight and yield) and flesh-quality (fillet fat content) traits in yellowtail kingfish Seriola lalandi. The observed major deformities included lower jaw, nasal erosion, deformed operculum and skinny fish on 480 individuals from 22 families at Clean Seas Tuna Ltd. They were typically recorded as binary traits (presence or absence) and were analysed separately by both threshold generalized models and standard animal mixed models. Consistency of the models was evaluated by calculating simple Pearson correlation of breeding values of full-sib families for jaw deformity. Genetic and phenotypic correlations among traits were estimated using a multitrait linear mixed model in ASReml. Both threshold and linear mixed model analysis showed that there is additive genetic variation in the four measures of deformity, with the estimates of heritability obtained from the former (threshold) models on liability scale ranging from 0.14 to 0.66 (SE 0.32-0.56) and from the latter (linear animal and sire) models on original (observed) scale, 0.01-0.23 (SE 0.03-0.16). When the estimates on the underlying liability were transformed to the observed scale (0, 1), they were generally consistent between threshold and linear mixed models. Phenotypic correlations among deformity traits were weak (close to zero). The genetic correlations among deformity traits were not significantly different from zero. Body weight and fillet carcass showed significant positive genetic correlations with jaw deformity (0.75 and 0.95, respectively). Genetic correlation between body weight and operculum was negative (-0.51, P < 0.05). The genetic correlations' estimates of body and carcass traits with other deformity were not significant due to their relatively high standard errors. Our results showed that there are prospects for genetic selection to improve deformity in yellowtail kingfish and that measures of deformity should be included in the recording scheme, breeding objectives and selection index in practical selective breeding programmes due to the antagonistic genetic correlations of deformed jaws with body and carcass performance. © 2016 John Wiley & Sons Ltd.


Whatmore P.,University of The Sunshine Coast | Whatmore P.,Australian Seafood Cooperative Research Center | Nguyen N.H.,University of The Sunshine Coast | Miller A.,Clean Seas Tuna Ltd | And 6 more authors.
Aquaculture | Year: 2013

The aim of the present study was to estimate genetic parameters for body and carcass traits, visual condition score, and deformity in yellowtail kingfish Seriola lalandi, an emerging aquaculture species in Australia. These novel data and genetic parameters are required to solve the problem of how to conduct efficient selection in this and related species. Analyses were performed on a total of 400 data records collected from a yellowtail kingfish breeding population at Cleanseas Tuna Ltd. farm. They were progeny of 22 full- and half-sib families (eight sires and six dams). Six newly developed and four published microsatellite markers were used to construct the pedigree. Genetic parameters were estimated using average information algorithm in ASReml with a multiple trait model. Fixed effects included sex, seal bite and deformity status. Random effects were the additive genetics of individual animal, and maternal and common environmental effects (i.e., dam-tank effect arising from a short period of separate rearing of offspring that came from two different broodstock tanks). The estimates of heritability for body and carcass traits were moderate (h2=0.15 to 0.30, s.e. ranging from 0.09 to 0.19). Fillet fat content showed an unusually high heritability (0.94±0.21) with a standard animal model, but was only moderate (0.41±0.26) when tank and dam were included as random effects. The estimate for condition score was 0.15±0.11, whereas the heritability for deformity was close to zero (h2=0.02). The genetic correlations between body and carcass (fillet weight and fillet yield) traits were high and positive (0.57 to 0.94, s.e. 0.05 to 0.46). Genetic correlations between body traits and condition score were moderate to high and positive (i.e. favourable). These results suggest that selection for high growth would result in concomitant increase in fillet weight, a carcass trait of paramount importance. It is concluded that there is substantial potential for genetic improvement of economically important traits especially growth performance and fillet weight in the current population of yellowtail kingfish. © 2013 Elsevier B.V.


Sanchis-Benlloch P.J.,University of The Sunshine Coast | Nocillado J.,University of The Sunshine Coast | Ladisa C.,University of The Sunshine Coast | Aizen J.,University of The Sunshine Coast | And 5 more authors.
General and Comparative Endocrinology | Year: 2016

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh. © 2016 Elsevier Inc.


Bar I.,University of The Sunshine Coast | Smith A.,University of The Sunshine Coast | Bubner E.,Flinders University | Yoshizaki G.,Tokyo University of Marine Science and Technology | And 5 more authors.
Reproduction, Fertility and Development | Year: 2016

Germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. The present study explored the suitability of yellowtail kingfish (Seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (Thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. Germ cell populations in testes of donor T. maccoyii males were described using basic histology and the molecular markers vasa and dead-end genes. The peripheral area of the testis was found to contain the highest proportions of dead-end-expressing transplantable Type A spermatogonia. T. maccoyii Type A spermatogonia-enriched preparations were transplanted into the coelomic cavity of 6-10-day-old post-hatch S. lalandi larvae. Fluorescence microscopy and polymerase chain reaction analysis detected the presence of tuna cells in the gonads of the transplanted kingfish fingerlings at 18, 28, 39 and 75 days after transplantation, indicating that the transplanted cells migrated to the genital ridge and had colonised the developing gonad. T. maccoyii germ cell-derived DNA or RNA was not detected at later stages, suggesting that the donor cells were not maintained in the hosts' gonads. © The Authors 2016.


Knibb W.,University of The Sunshine Coast | Miller A.,Clean Seas Tuna Ltd | Quinn J.,University of The Sunshine Coast | D'Antignana T.,Clean Seas Tuna Ltd | Nguyen N.H.,University of The Sunshine Coast
Aquaculture | Year: 2016

Recently, aquaculture and captive breeding have commenced for a raft of large fish species, including bluefin tuna (Thunnus orientalis), kingfish (Seriola lalandi) and giant grouper (Epinephelus lanceolatus). With captive breeding, there is often interest to conduct selection and genetic improvement, but these large species present many and substantial challenges to selective breeding. Indeed there are no reports or examples that selection response and forward genetic gain has been achieved for such large and problematic species. These large species, typified by kingfish, are characterized by immense fecundity with ensuing opportunity for intense domestication selection, either adverse or synergistic, that can impact on planned selection response. Moreover, because of size and logistics, typically few broodstock are held and chance sampling of few broodstock individuals has the potential to confound selection response and the repeatability of response.The main objective here was to assess if forward selection response could be achieved after selection for adult weight of kingfish in sea cages. Selection response was estimated by comparing the performance of F1 offspring from wild parents with F2 offspring from selected parents during the larval rearing and adult growout. Pedigree data, from genotyping approximately 1000 individuals using up to 17 DNA microsatellite loci, was added to the larval and adult performance data to resolve the contributions of different sire and dam lineages.For most traits measured, whether larval survival, incidence of larval deformity, adult weights or adult condition factor, the offspring of selected parents outperformed those from wild parents, whether animals were grown separately in replicate (larval rearing tanks) or communally as adults in tanks. Larval survival was not deliberated selected, yet the dropout of specific parental sire or dam lineages suggest in part genetic differences account for some of the differences between the F1 and F2. Observed selection responses for adult weights and condition factor were greater than those predicted from covariances of relatives. Either (synergistic) domestication selection or some type of magnification of line differences under communal rearing may account for these data.The chance sampling of particularly good or bad broodstock sires or dams did not seem to have been a major contributor to the strain testing results. Whether this means that selection responses are repeatable even when using few broodstock, a situation unavoidable for large marine species, is discussed in terms of how intense deliberate and possible domestication selection could narrow the variances of breeding values of selected broodstock. Statement of relevance: Can we select large marine species with few broodstock? © 2015 Elsevier B.V.


PubMed | University of The Sunshine Coast and Clean Seas Tuna Ltd
Type: Journal Article | Journal: Journal of fish diseases | Year: 2016

The main aim of this study was to estimate the heritability for four measures of deformity and their genetic associations with growth (body weight and length), carcass (fillet weight and yield) and flesh-quality (fillet fat content) traits in yellowtail kingfish Seriola lalandi. The observed major deformities included lower jaw, nasal erosion, deformed operculum and skinny fish on 480 individuals from 22 families at Clean Seas Tuna Ltd. They were typically recorded as binary traits (presence or absence) and were analysed separately by both threshold generalized models and standard animal mixed models. Consistency of the models was evaluated by calculating simple Pearson correlation of breeding values of full-sib families for jaw deformity. Genetic and phenotypic correlations among traits were estimated using a multitrait linear mixed model in ASReml. Both threshold and linear mixed model analysis showed that there is additive genetic variation in the four measures of deformity, with the estimates of heritability obtained from the former (threshold) models on liability scale ranging from 0.14 to 0.66 (SE 0.32-0.56) and from the latter (linear animal and sire) models on original (observed) scale, 0.01-0.23 (SE 0.03-0.16). When the estimates on the underlying liability were transformed to the observed scale (0, 1), they were generally consistent between threshold and linear mixed models. Phenotypic correlations among deformity traits were weak (close to zero). The genetic correlations among deformity traits were not significantly different from zero. Body weight and fillet carcass showed significant positive genetic correlations with jaw deformity (0.75 and 0.95, respectively). Genetic correlation between body weight and operculum was negative (-0.51, P < 0.05). The genetic correlations estimates of body and carcass traits with other deformity were not significant due to their relatively high standard errors. Our results showed that there are prospects for genetic selection to improve deformity in yellowtail kingfish and that measures of deformity should be included in the recording scheme, breeding objectives and selection index in practical selective breeding programmes due to the antagonistic genetic correlations of deformed jaws with body and carcass performance.


Trademark
Clean Seas Tuna Ltd | Date: 2016-03-23

fish, not live, raised by aquaculture, namely, mulloway, kingfish and tuna.

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