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DELFT, Netherlands

Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: KBBE-2009-3-3-02 | Award Amount: 3.47M | Year: 2010

This project intends to engineer transaminase libraries that will be applied as the main enzymatic technology to deliver the amine functionality in the commercially valuable products of both chiral and bulk amine targets. These enzymes will be used in enzymatic cascades where simple starting materials are converted into the required intermediates for transamination or further enzymatic steps will be used to remove products from the transaminase reaction which will add value by extra functionality. This will also be supported by the development of enzymatic cascades to deliver efficient co-factor recycling and achieve the high conversions required for industrial use. A high throughput screening method based on a further enzymatic cascade will be developed. Engineering solutions will be used to overcome obstacles associated with the implementation of this core technology on a larger scale and integrate the use of other enzymes into the synthetic pathway to allow multi-step, multi-enzyme cascades to be used to deliver complex multi-functional amine products and processes. The industrial partner will target the development of enzymes from the project for specific application into their new products range. Life cycle analysis and environmental impact analysis will compare the final methods with conventional chemical synthesis and allow advantages to be objectively defined.

Agency: Cordis | Branch: FP7 | Program: CP-TP | Phase: KBBE.2013.3.3-04 | Award Amount: 11.20M | Year: 2013

The INDOX proposal on industrial oxidoreductases aims to provide relevant industrial case stories to demonstrate the efficacy of optimized biocatalysts on targeted reactions, and to establish the processes scalability, sustainability and cost-efficiency versus chemical conversion processes. The chemical industry (specialties excluded) is not yet embracing enzymatic oxidation reactions to a significant extent primarily due to lack of biocatalysts with the required selectivity, availability and compatibility with the rigorous process conditions. Selected industrial oxidation and oxyfunctionalization target reactions form the basis for the INDOX screening and optimization of new biocatalysts, including: i) Intermediates for agrochemicals/APIs; ii) Polymer precursors and functionalized polymers; and iii) Intermediates for dye-stuffs. The project flow comprises: i) Recovery of selective biocatalysts from the groups of heme-peroxidases/peroxygenases, flavo-oxidases and copper-oxidoreductases from fungal genomes and other sources; ii) Improvement of their oxidative activity and stability by protein engineering (using rational design, directed evolution and hybrid approaches combined with computational calculations) to fulfill the operational and catalytic conditions required by the chemical industry; and iii) Optimization of reaction conditions and reactor configurations (including immobilization technologies and new enzymatic cascade reactions). Finally the cost efficiency compared to chemical processing will be evaluated. The INDOX approach is supported by a highly-specialized consortium of SMEs, large companies and research/academic institutions. Production of the new optimized biocatalysts and their introduction into the chemical market will take advantage from the participation of the world-leading company in the sector of industrial enzymes, together with several chemical companies willing to implement the new medium- and large-scale biotransformation processes.

Agency: Cordis | Branch: FP7 | Program: CP-TP | Phase: KBBE.2011.3.4-02 | Award Amount: 13.25M | Year: 2012

BioConSepT aims to demonstrate the technically feasibility of White Biotech processes for the conversion of 2nd generation biomass into platform chemicals, which are 30% cheaper and 30% more sustainable than existing chemical routes or 1st generation processes. BioConSepT uses lignocellulose and non-edible oils & fats as cheap, abundantly available feedstocks, which cannot be used as food. The main achievements expected for BioConSepT are: (1) to develop the robust enzymes and micro-organisms suited for the more dirty 2nd generation feedstocks; (2) to reduce equipment costs and the number of process steps by the integration of bioconversion and highly selective separation technologies; (3) to facilitate easy integration in existing production chains by deploying combinations of bio- and chemical conversions and by proving the suitability of the produced platform chemicals for bio-based polymers, resins, plasticizers, solvents and surfactants and (4) by realisation of the 1st demonstration of integrated production chains from 2nd generation feedstocks to platform chemicals at industrially relevant scale. BioConSepT will bring novel technologies from lab to pilot scale by high level applied research. The consortium consists of 15 SMEs (suppliers of equipment, bioconversions, separation technologies and services), 10 large industrial parties (producers, end-users, engineering and consultancy companies) and 5 leading RTOs from 11 different countries. The large industrial parties and SMEs expect new products, processes, services and customers with a potential value of hundreds of M. BioConSepT will reduce the total processing costs and thus improve the competitiveness of the European agro/food and chemical industries. The use of renewable biomass will lead to a significant reduction of Green House Gas emissions and a more secure supply of feedstocks, energy and water as well as reduction of waste generation.

Agency: Cordis | Branch: FP7 | Program: CP-TP | Phase: KBBE.2011.3.3-02 | Award Amount: 7.77M | Year: 2011

The objective of KYROBIO project is to broaden the toolbox of single enantiomer chiral chemicals that are produced by industry in Europe using biotechnological routes. The main target is applications of lyase enzymes to selectively synthesize molecules with multiple chiral centres applying enzymatic carbon-carbon and carbon-nitrogen bond formation as the key technical platforms. We will then apply synthetic biology to improve fermentation processes in order to generate better enzymes. Chiral compounds are an important class of chemicals that biocatalytic transformation has already demonstrated great potential to compete with chemocatalysts in their production with associated benefits that come from reductions in use of organic solvents, toxic metals and energy but application has been relatively limited. KYROBIO will address the main challenges with moving forward to the next generation of added value industrial applications of white biotechnology for high value chemical synthesis. Using a supradisciplinary approach ranging from enzyme development, chemistry, molecular biology, fermentation and innovative isolation techniques the bottlenecks to applying this new technology will be overcome. It is expected that promising candidate chemicals will be commercialised within three years of completion and so scale up with economic and feasibility studies that are also key technology developments. The consortium includes a strong presence of SMEs including SME leadership and also a large multinational company which ensures multiple routes to market for the outcomes of this project. We will also have economic and life cycle analysis coupled with significant dissemination plans to ensure wider understanding of this technology that will lead to increased acceptance and uptake. The use of this environmentally beneficial technology will help to keep the European chemicals industry at the forefront of white biotechnology and increase opportunities in economic and employment.

Agency: Cordis | Branch: FP7 | Program: CP-TP | Phase: KBBE.2013.3.3-04 | Award Amount: 7.41M | Year: 2013

Currently the aerobic bio-catalytic oxidation reaction is the one that would have the biggest impact on the future uptake of industrial biotechnology in Europe. Chemical oxidation is both hazardous and has high environment impacts. Many oxidative bio-catalytic reactions and transformations have been identified in academic laboratories but only a very small number have been applied by industry to oxidize non-natural substrates. Hence biocatalysis for oxidative chemical manufacture processes can deliver a major advantage to the European chemical-using industries and the environment. In this project we intend to develop the tools for implementation of bio-oxidation to synthesize and oxidize alcohols. Cytochrome P450 enzymes will be investigated for hydroxylation of fatty acid derivatives and terpenes which have potential to be used in biopolymers and fragrance chemicals respectively form one line of investigation. The second is the selective oxidation of primary alcohols to give products with added value in their own right and as intermediates towards other valuable products. To support the implementation culture collections and literature reported DNA sequences will be used to identify diverse enzymes with predicted oxidase activities, which will be used as starting point for an enzyme improvement program. Then fermentation and enzyme formulation techniques will improve reaction performance to a level where useful quantities of target products can be produced for evaluation by industrial partners, and engineering techniques will analyse and implement reactor configurations that will further improve this technology platform to enable this technology to be introduced as a routine technology in the IB industry and support the European KBBE. Further a dynamic public engagement and dissemination program will be used to promote the project, IB and the FP7 program within the science community and the public, especially schoolchildren, to create extra value for the funders.

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