Ck Thakur Acs College

Raigarh Fort, India

Ck Thakur Acs College

Raigarh Fort, India
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Mhatre M.A.,Ck Thakur Acs College
Journal of Ecophysiology and Occupational Health | Year: 2015

The etoposide (VP-16) is in use as one of the regimen in cyclical chemotherapeutic treatment for several kind of cancer like testicular cancer, small cell bronchogenic carcinoma, malignant lymphoma. Etoposide (VP-16), an anticancer drug, 1mg/kg/day was administered intramuscular for two months. The animals were anaesthetized with ether and excised testis and epididymis for electron microscopic studies. The testicular tissue revealed that the severe atrophic changes, suggestive arrest at the germinal cell level as well as at the Sertoli cell level. The cellular integrity of all is drastically reduced with a severe nuclear alteration also taking place. The observations showed that damage of early spermatogonia and the later stages of spermatogenesis were completely eliminated. The leydig cells revealed state of work induced hypertrophy as is evident from the secretory activity but the organelles show a condition of extreme overburdened state just prior to state of collapse. In conclusion our result shows that adverse effect of etoposide on testis especially on spermatogonia can be distinguished after two months treatment. The electron microscopic features show that it leads towards the male fertility disorder. © 2015 The Academy of Environmental Biology, India.


Mhatre M.A.,Ck Thakur Acs College
Journal of Ecophysiology and Occupational Health | Year: 2015

To study ultra-structure changes in epididymis caused by an etoposide(VP-16) with the help of electron microscope. The etoposide (VP-16) is in use as one of the regimen in cyclical chemotherapeutic treatment for several kind of cancer. Etoposide as an anticancer drug, 1mg/kg/day was administered intramuscular for two months. The animals were anaesthetized with ether and excised testis and epididymis for electron microscopic studies. Etoposide treatment resulted. The goal of theses study was to determine. In Caput epididymis as compared with the control cell, the supranuclear cytoplasm shows hypertrophied, dilated and distorted Golgi cisternae and vesicles are filled with moderately dense material. The Principal cells of epididymis shows increase in abudance, as well as size of lysosomal bodies and their appearances in the basal cytoplasm in the principal cell of the caput and cauda epididymis indicate argumented endocytosis. In the present study it has been shown that the vesicle dilated due to treatment with etoposide contain an electron dense material providing evidence for formation of secretory granules enclosed in membranous vesicles in the transfer of the golgi apparatus of principal cells. The vesiculation/ vaculation of golgi apparatus to the opening up of golgi area observed in the principal cell of etoposide treated rat because a toxic manifestation of the golgi apparatus as a heightened activity. In conclusion our result shows that adverse effect of etoposide on epididymis can be distinguished after two months treatment. © 2015 The Academy of Environmental Biology, India.


Patil S.K.,Ck Thakur Acs College | Pisal R.H.,Ck Thakur Acs College | Deokar H.P.,Ck Thakur Acs College
Der Pharma Chemica | Year: 2014

The aim of present scheme is to synthesize of new phthalamide heterocycles and elucidates their biological activity. For the synthesis, phthalamide molecule was treated with various reagents as per the requirement of reaction and every time it result in to the formation of novel derivative of phthalamide. Derivatives obtained were bromo, benzimidazol, isothiocyanate, acetyl, benzothiazol, isothiocyanate and diazepin. Obtained derivatives further studied for their characterization using spectral techniques like IR, NMR and elemental analysis which established the structure of derivative. Derivatives tested for their antibacterial activity against gram positive and gram negative bacterial strains like E. coli and S. aureus.

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