Enrichi F.,CIVEN and Nanofab |
Ricco R.,CIVEN and Nanofab |
Meneghello A.,CIVEN and Nanofab |
Pierobon R.,CIVEN and Nanofab |
And 7 more authors.
Optical Materials | Year: 2010
DNA microarray is a high-throughput technology used for detection and quantification of nucleic acid molecules and others of biological interest. The analysis is resulting by specific hybridization between probe sequences deposited in array and a target ss-DNA usually functionalized by a luminescent dye. These organic labels have well known disadvantages like photobleaching and limited sensitivity. Therefore in this work we investigate a different strategy, based on the use of inorganic silica nanospheres incorporating standard luminescent dyes or rare earth ions. The synthesis and characterization of these biomarkers is reported and their application to the DNA microarray technology in comparison to the use of standard molecular fluorophores or commercial quantum dots is discussed. We show that dye doped silica spheres provides a significant increase of the optical emission signal with respect to the use of free dyes, while rare earth doped silica spheres allow reducing or completely avoiding the background noise. These aspects, together with their cheap and easy synthesis, stability in water, easy surface functionalization and bio-compatibility makes them very promising for present and future applications in bio-labelling and bio-imaging. © 2010 Elsevier B.V. All rights reserved.
Moretti E.,University of Venice |
Storaro L.,University of Venice |
Chessa G.,University of Venice |
Talon A.,University of Venice |
And 4 more authors.
Journal of Colloid and Interface Science | Year: 2012
Here we describe the step-wise grafting of the fluorophore dansyl chloride on the interlayer aluminol groups of kaolinite. The modified clay was characterized by powder RD, TGA, FT-IR and 27Al, 19Si, 13C MAS-NMR, which confirmed the achievement of the clay functionalization. The photophysical properties of the resulting nanohybrid material were evaluated by photoluminescence excitation and emission measurements. © 2012 Elsevier Inc.