City of Scientific Research and Technology Applications

Alexandria, Egypt

City of Scientific Research and Technology Applications

Alexandria, Egypt
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Shaaban M.,Bahauddin Zakariya University | Abid M.,Bahauddin Zakariya University | Abou-Shanab R.A.I.,City of Scientific Research and Technology Applications
Plant, Soil and Environment | Year: 2013

Combined application of organic and inorganic amendments was known to play a significant role in improvement of soil properties. A field experiment was conducted to explore the effects of gypsum, farmyard manure and commercial humic acid application on the amelioration of salt affected (saline sodic) soil. During this study, soil pH, electrical conductivity, sodium adsorption ratio, responses of root length and rice paddy yield were examined. Application of gypsum with or without farm manure and commercial humic acid decreased soil pH (8.26%), electrical conductivity (from 6.35 dS/m to 2.65 dS/m) and sodium adsorption ratio (from 26.56 to 11.60), and increased root length (from 9.17 cm to 22.6 cm) and paddy yield (from 695.7 kg/ha to 1644 kg/ha). A negative but significant correlation was observed between root length and electrical conductivity, sodium adsorption ratio (r = -0.93; -0.94, respectively, P ≤ 0.05), whereas positive and significant correlation with paddy yield (r = 0.96; P ≤ 0.05).

Ji M.-K.,Yonsei University | Kim H.-C.,Pennsylvania State University | Sapireddy V.R.,Yonsei University | Yun H.-S.,Yonsei University | And 6 more authors.
Applied Microbiology and Biotechnology | Year: 2013

The feasibility of using a microalga Chlorella vulgaris YSW-04 was investigated for removal of nutrients from piggery wastewater effluent. The consequent lipid production by the microalga was also identified and quantitatively determined. The wastewater effluent was diluted to different concentrations ranging from 20 to 80 % of the original using either synthetic media or distilled water. The dilution effect on both lipid production and nutrient removal was evaluated, and growth rate of C. vulgaris was also monitored. Dilution of the wastewater effluent improved microalgal growth, lipid productivity, and nutrient removal. The growth rate of C. vulgaris was increased with decreased concentration of piggery wastewater in the culture media regardless of the diluent type. Lipid production was relatively higher when using synthetic media than using distilled water for dilution of wastewater. The composition of fatty acids accumulated in microalgal biomass was dependent upon both dilution ratio and diluent type. The microalga grown on a 20 % concentration of wastewater effluent diluted with distilled water was more promising for generating high-efficient biodiesel compared to the other culture conditions. The highest removal of inorganic nutrients was also achieved at the same dilution condition. Our results revealed the optimal pretreatment condition for the biodegradation of piggery wastewater with microalgae for subsequent production of high-efficient biodiesel. © 2012 Springer-Verlag.

PubMed | Ain Shams University and City of Scientific Research and Technology Applications
Type: Journal Article | Journal: Bulletin of environmental contamination and toxicology | Year: 2016

A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Of the 20 isolates, RAM03, RAM06, RAM13, and RAM17 were specifically chosen based on their relatively higher growth on salt medium amended with 4% crude oil, emulsion index, surface tension, and degradation percentage. These bacterial cultures had 16SrRNA gene sequences that were most similar to Ochrobactrum cytisi (RAM03), Ochrobactrum anthropi (RAM06 and RAM17), and Sinorhizobium meliloti (RAM13) with 96%, 100% and 99%, and 99% similarity. The tested strains revealed a promising potential for bioremediation of petroleum oil contamination as they could degrade >93% and 54% of total petroleum hydrocarbons (TPHs) in a liquid medium and soil amended with 4% crude oil, respectively, after 30day incubation. These bacteria could effectively remove both aliphatic and aromatic petroleum hydrocarbons. In conclusion, these strains could be considered as good prospects for their application in bioremediation of hydrocarbon contaminated environment.

Ji M.-K.,Yonsei University | Abou-Shanab R.A.I.,Yonsei University | Abou-Shanab R.A.I.,City of Scientific Research and Technology Applications | Kim S.-H.,Yonsei University | And 6 more authors.
Ecological Engineering | Year: 2013

This study demonstrates the potential for algae-based biofuel production by coupling advanced wastewater treatment with microalgae cultivation for low-cost lipid production. Three species (Chlorella vulgaris, Scenedesmus obliquus and Ourococcus multisporus) with higher biomass yield were selected and cultured in wastewater amended with 15% CO2. C. vulgaris, S. obliquus and O. multisporus showed optimal specific growth rates (μopt) of 1.37, 1.14 and 1.00 day-1, respectively, and almost complete removal (>99%) of nitrogen and phosphorus within 4 days. The highest specific lipid productivity was 0.164g-lipidsg-cell-1 day-1 and oleic acid was increased to 44% in C. vulgaris after 7 days of cultivation in the presence of CO2. It was concluded that C. vulgaris is a good potential source for the production of biodiesel coupled with nutrient removal from wastewater. © 2013 Elsevier B.V.

PubMed | Al - Azhar University of Egypt, Mansoura University and City of Scientific Research and Technology Applications
Type: | Journal: Journal of pharmaceutical and biomedical analysis | Year: 2016

A simple, sensitive and rapid high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for the simultaneous quantitation of clopamide, reserpine and dihydroergotoxine (ergoloid mesylates) in human plasma. Under basic conditions, liquid-liquid extraction using ethyl acetate was efficiently used for extraction of the analytes from plasma samples in presence of indapamide as internal standard (IS). The analytes were separated with isocratic elution on Phenomenex() Synergi Fusion-RP 80A column (504.6mm, 4m). With positive ion electrospray ionization (ESI), the analytes were quantified and monitored on a triple quadrupole mass spectrometer using Multiple Reaction Monitoring (MRM) scanning mode. Satisfactory results regarding linearity, recovery, stability, accuracy and precision of the analytes were obtained. The method was linear in the concentration range of 0.04-30.00ng/mL for reserpine, 1-96.00ng/mL for clopamide, and 0.05-40.00ng/mL for dihydroergotoxine alkaloids, respectively. For all analytes, the high sensitivity of HPLC-MS/MS method revealed sufficient lower limit of quantification (LLOQ) ranged from 0.04-1ng/mL using 1mL of plasma. The recoveries from spiked control samples were 86.16% for all analytes and IS. The intra- and inter-day precision variations were lower than 13.03% while the accuracy values ranged from 91.76% to 111.50%. The developed method was successfully applied to pharmacokinetic study of fixed dose combination of clopamide, reserpine and dihydroergotoxine in healthy male volunteers.

PubMed | Advanced Technology & New Materials Research Institute, City of Scientific Research and Technology Applications and Genetic Engineering and Biotechnology Research Institute
Type: Journal Article | Journal: Applied biochemistry and biotechnology | Year: 2016

In this study, we synthesized tungsten oxide (WO

PubMed | Hanyang University, City of Scientific Research and Technology Applications and Korea Institute of Science and Technology
Type: | Journal: Bioresource technology | Year: 2016

This study evaluated the toxicity and cellular stresses of carbamazepine (CBZ) on Chlamydomonas mexicana and Scenedesmus obliquus, and its biodegradation by both microalgal species. The growth of both microalgal species decreased with increase of CBZ concentration. The growth of S. obliquus was significantly inhibited (97%) at 200mgCBZL(-1), as compared to the control after 10days; whereas, C. mexicana showed 30% inhibition at the same experimental conditions. Biochemical characteristics including total chlorophyll, carotenoid contents and enzyme activities (SOD and CAT) for both species were affected by CBZ at relatively high concentration. C. mexicana and S. obliquus could achieve a maximum of 35% and 28% biodegradation of CBZ, respectively. Two metabolites (10,11-dihydro-10,11-expoxycarbamazepine and n-hydroxy-CBZ) were identified by UPLC-MS, as a result of CBZ biodegradation by C. mexicana. This study demonstrated that C. mexicana was more tolerant to CBZ and could be used for treatment of CBZ contaminated wastewater.

El-Sherbiny I.M.,Center for Materials Science | Salih E.,Center for Materials Science | Yassin A.M.,Genetic Engineering and Biotechnology Research Institute | Hafez E.E.,City of Scientific Research and Technology Applications
Journal of Nanoparticle Research | Year: 2016

Abstract: The present study reports the biosafety assessment, the exact molecular effects, and apoptosis induction of newly developed chitosan-silver hybrid nanoparticles (Cs–Ag NPs) in HepG2 cells. The investigated hybrid NPs were green synthesized using Cs/grape leaves aqueous extract (Cs/GLE) or Cs/GLE NPs as reducing and stabilizing agents. The successful formation of Cs/GLE NPs and Cs–Ag hybrid NPs has been confirmed by UV–Vis spectrophotometry, FTIR spectroscopy, XRD, and HRTEM. From the TEM analysis, the prepared Cs/GLE NPs are uniform and spherical with an average size of 150 nm, and the AgNPs (5–10 nm) were formed mainly on their surface. The UV–Vis spectra of Cs–Ag NPs showed a surface plasmon resonance (SPR) peak at about 450 nm confirming their formation. The synthesized Cs–Ag NPs were found to be crystalline as shown by XRD patterns with fcc phase oriented along the (111), (200), (220), and (311) planes. The cytotoxicity patterns, the antiproliferative activities, and the possible mechanisms of anticancer activity at molecular level of the newly developed Cs–Ag hybrid NPs were investigated. Cytotoxicity patterns of all the preparations demonstrated that the nontoxic treatment concentrations are ranged from 0.39 to 50 %, and many of the newly prepared Cs–Ag hybrid NPs showed high anticancer activities against HpG2 cells, and induced cellular apoptosis by downregulating BCL2 gene and upregulating P53. Graphical Abstract: [Figure not available: see fulltext.] © 2016, Springer Science+Business Media Dordrecht.

Salama E.-S.,Yonsei University | Kim H.-C.,Yonsei University | Abou-Shanab R.A.I.,Yonsei University | Abou-Shanab R.A.I.,City of Scientific Research and Technology Applications | And 4 more authors.
Bioprocess and Biosystems Engineering | Year: 2013

Two freshwater microalgae including Chlamydomonas mexicana and Scenedesmus obliquus were grown on Bold Basal Medium (BBM) with different levels of salinity up to 100 mM NaCl. The dry biomass and lipid content of microalgae were improved as the concentration of NaCl increased from 0 to 25 mM. Highest dry weight (0.8 and 0.65 g/L) and lipid content (37 and 34 %) of C. mexicana and S. obliquus, respectively, were obtained in BBM amended with 25 mM NaCl. The fatty acid composition of the investigated species was also improved by the increased NaCl concentration. At 50 mM, NaCl palmitic acid (35 %) and linoleic acid (41 %) were the dominant fatty acids in C. mexicana, while oleic acid (41 %) and α-linolenic acid (20 %) were the major fractions found in S. obliquus. © 2013 Springer-Verlag Berlin Heidelberg.

Abou-Shanab R.A.I.,Yonsei University | Abou-Shanab R.A.I.,City of Scientific Research and Technology Applications | Ji M.-K.,Yonsei University | Kim H.-C.,Yonsei University | And 2 more authors.
Journal of Environmental Management | Year: 2013

Six microalgal species were examined in this study to determine their effectiveness in the coupling of piggery wastewater treatment and biodiesel production. The dry biomasses of Ourococcus multisporus, Nitzschia cf. pusilla, Chlamydomonas mexicana, Scenedesmus obliquus, Chlorella vulgaris, and Micractinium reisseri were 0.34 ± 0.08, 0.37 ± 0.13, 0.56 ± 0.35, 0.53 ± 0.30, 0.49 ± 0.26, and 0.35 ± 0.08 g dwt/L, respectively. The highest removal of nitrogen (62%), phosphorus (28%), and inorganic carbon (29%) were achieved by C. mexicana. In the absence of microalgae, the spontaneous precipitation of phosphorus, calcium, and inorganic carbon occurred at slightly alkaline pH. The highest lipid productivity and lipid content (0.31 ± 0.03 g/L and 33 ± 3%, respectively) were found in C. mexicana. The fatty acid compositions of the studied species were mainly palmitic, linoleic, α-linolenic, and oleic. The results of our study suggest that C. mexicana is one of the most promising candidates for simultaneous nutrient removal and high-efficient biodiesel production. © 2012 Elsevier Ltd.

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