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Ito T.,National Institute of Fruit Tree Science NIFTS | Ohta S.,Okitsu Citrus Research Station
Journal of General Plant Pathology | Year: 2010

We tested citrus samples from Japan using reverse transcription-polymerase chain reaction (RT-PCR), and for the first time, Citrus viroid V (CVd-V) was detected in Japan. Using nucleotide sequence analysis, we identified two new CVd-V variants, which shared 91-96% nucleotide sequence identities with those reported previously and 89% with each other. The RT-PCR detected CVd-V from 44 of 275 citrus trees tested, showing that CVd-V can be spread to some extent within commercial orchards in Japan. © 2010 The Phytopathological Society of Japan and Springer.

Nakaune R.,Japan National Agriculture and Food Research Organization | Tatsuki M.,1 Fujimoto | Matsumoto H.,Okitsu Citrus Research Station | Ikoma Y.,Okitsu Citrus Research Station
Journal of General Plant Pathology | Year: 2015

A brown skin spot developed on the berries of Japanese grape cultivar Shine Muscat during storage at 0 °C for 3 months. The fungus isolated from these skin spots was identified as Cadophora luteo-olivacea based on the sequence of its ribosomal DNA internal transcribed spacer region. Morphological and culture characteristics corresponded to those reported previously from C. luteo-olivacea; this fungus also reproduced the same symptoms after inoculation and was reisolated from the symptomatic spots. This is the first report of a postharvest disease of grape caused by C. luteo-olivacea. © 2015 The Phytopathological Society of Japan and Springer Japan

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